LOCUS       Exported                7535 bp ds-DNA     circular SYN 30-APR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 27, 2021 from SnapGene Viewer 5.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..7535
                     /label=pEM791
     misc_feature    1..7535
                     /label=RMCE_CAG-3UTR
     misc_feature    1..311
                     /label=TKpA
     misc_feature    312..345
                     /label=Lox2271
     CDS             350..949
                     /codon_start=1
                     /label=Pur
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    459..5145
                     /label=pEM705
     misc_feature    1097..1116
                     /label=bGH_3'UTR_seq2
     misc_feature    1181..1186
                     /label=pA
     misc_feature    1285..3000
                     /label=CAG promoter
     CDS             3029..3724
                     /codon_start=1
                     /label=mCherry
                     /note="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMD"
     CDS             3743..4042
                     /codon_start=1
                     /label=INBox: INCENP 819-918
                     /note="INCENP without its N-terminal IN box"
                     /translation="PDNYGMDLNSDDSTDDEAHPRKPIPTWARGTPLSQAIIHQYYHPP
                     NLLELFGTILPLDLEDIFKKSKPRYHKRTSSAVWNSPPLQGARVPSSLAYSLKKH"
     misc_feature    3749..4042
                     /label=IN-box
                     /note="this region is responsible for binding and 
                     activating Aurora B"
     CDS             4070..4543
                     /codon_start=1
                     /label=eDHFR
                     /note="residues 2-159"
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             4544..4572
                     /codon_start=1
                     /label=myc
                     /note="myc"
                     /translation="EQKLISEED"
     misc_feature    4573..4576
                     /label=IRES PCR 398 399
     misc_feature    4577
                     /label=HiLo_MCS_219-220
     misc_feature    4578..4584
                     /label=HiLo_MCS_219-220
     misc_feature    4592..5140
                     /label=globin 3'UTR
     misc_feature    5163..5196
                     /label=LoxP
     misc_feature    5790..6323
                     /label=ORI
     misc_feature    complement(6549..7409)
                     /label=Bla (AmpR)
     misc_feature    complement(7410..7508)
                     /label=Bla promoter
ORIGIN
        1 aagcttaata aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
       61 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
      121 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
      181 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
      241 gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca
      301 ttcgagaata aataacttcg tataaagtat cctatacgaa gttatcacca tgaccgagta
      361 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
      421 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
      481 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
      541 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
      601 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
      661 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
      721 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
      781 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
      841 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
      901 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
      961 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat ggctccgacc gaagccgacc
     1021 cgggcggccc cgccgacccc gcacccgccc ccgaggccca ccgactctag agctcgctga
     1081 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1141 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1201 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     1261 ggggaggatt gggaagacaa tagcactagt tattaatagt aatcaattac ggggtcatta
     1321 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     1381 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     1441 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     1501 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     1561 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1621 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     1681 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     1741 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc
     1801 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1861 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1921 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1981 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     2041 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     2101 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     2161 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     2221 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     2281 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     2341 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     2401 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     2461 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     2521 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     2581 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     2641 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     2701 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     2761 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     2821 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2881 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2941 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     3001 ctcgaccacc ggcgcgccac cggtcaccat ggtgagcaag ggcgaggagg ataacatggc
     3061 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     3121 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     3181 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     3241 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     3301 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     3361 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     3421 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     3481 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     3541 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     3601 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     3661 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     3721 ggacgagctg tataagggta ccccagataa ctacgggatg gatctgaata gcgacgactc
     3781 caccgatgat gaggcccatc cccggaagcc catccccacc tgggcccgag gcaccccgct
     3841 cagccaggct atcattcacc agtactacca cccaccgaac cttctggagc tctttggaac
     3901 cattctccca ctggacttgg aggatatctt caagaagagc aagccccgct atcacaagcg
     3961 caccagctct gctgtctgga actcaccgcc cctgcagggc gccagggtcc ccagcagcct
     4021 ggcctacagc ctgaagaagc acggatccac cggatccctg gagtctagaa tcagtctgat
     4081 tgcggcgtta gcggtagatc gcgttatcgg catggaaaac gccatgccgt ggaacctgcc
     4141 tgccgatctc gcctggttta aacgcaacac cttaaataaa cccgtgatta tgggccgcca
     4201 tacctgggaa tcaatcggtc gtccgttgcc aggacgcaaa aatattatcc tcagcagtca
     4261 accgggtacg gacgatcgcg taacgtgggt gaagtcggtg gatgaagcca tcgcggcgtg
     4321 tggtgacgta ccagaaatca tggtgattgg cggcggtcgc gtttatgaac agttcttgcc
     4381 aaaagcgcaa aaactgtatc tgacgcatat cgacgcagaa gtggaaggcg acacccattt
     4441 cccggattac gagccggatg actgggaatc ggtattcagc gaattccacg atgctgatgc
     4501 gcagaactct cacagctatt gctttgagat tctggagcgg cgggaacaaa aactcatctc
     4561 agaagaggat ctttaattaa gcgctcgagg cggccgcaat tcactcctca ggtgcaggct
     4621 gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat
     4681 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     4741 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     4801 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     4861 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
     4921 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     4981 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     5041 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     5101 gctgtccctc ttctcttatg aagatccctc gacctgagcg tcgagctagc atatggatcc
     5161 atataacttc gtataatgta tgctatacga agttattacg ataccgtcaa cttgtttatt
     5221 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5281 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta ggtacccagc
     5341 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     5401 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     5461 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     5521 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     5581 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5641 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5701 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5761 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5821 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5881 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5941 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6001 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6061 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6121 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6181 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6241 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6301 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6361 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6421 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6481 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6541 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6601 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6661 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6721 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6781 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6841 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6901 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6961 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7021 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7081 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7141 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7201 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7261 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7321 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7381 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7441 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7501 ataggggttc cgcgcacatt tccccgaaaa gtgcc
//
