LOCUS       Exported                8458 bp ds-DNA     circular SYN 19-APR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Michael Lampson
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 27, 2021 from SnapGene Viewer 5.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..311
                     /label=TKpA
     misc_feature    312..345
                     /label=Lox2271
     CDS             350..949
                     /codon_start=1
                     /label=Pur
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    1091..1284
                     /label=bGH_3'UTR
     misc_feature    1291..3000
                     /label=CAG promoter
     primer_bind     3009..3052
                     /label=AgeI-MCAK truncated-F
                     /note="Primer for infusion cloning of MCAK (179-583) into 
                     plasmid #752 cut with AgeI."
     misc_feature    3009..3023
                     /label=Untitled
     CDS             3031..4245
                     /codon_start=1
                     /label=MCAK (Kif2C isoform 1)
                     /translation="SSSANPVNSVRRKSCLVKEVEKMKNKREEKKAQNSEMRMKRAQEY
                     DSSFPNWEFARMIKEFRATLECHPLTMTDPIEEHRICVCVRKRPLNKQELAKKEIDVIS
                     IPSKCLLLVHEPKLKVDLTKYLENQAFCFDFAFDETASNEVVYRFTARPLVQTIFEGGK
                     ATCFAYGQTGSGKTHTMGGDLSGKAQNASKGIYAMASRDVFLLKNQPCYRKLGLEVYVT
                     FFEIYNGKLFDLLNKKAKLRVLEDGKQQVQVVGLQEHLVNSADDVIKMIDMGSACRTSG
                     QTFANSNSSRSHACFQIILRAKGRMHGKFSLVDLAGNERGADTSSADRQTRMEGAEINK
                     SLLALKECIRALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIATISPGISSCEYTLN
                     TLRYAD"
     misc_feature    3031..4245
                     /label=pEM791
     misc_feature    3031..4245
                     /label=pEM705
     misc_feature    3031..4245
                     /label=RMCE_CAG-3UTR
     primer_bind     complement(4225..4272)
                     /label=AgeI-MCAK truncated-R
                     /note="Primer for infusion cloning of MCAK (179-583) into 
                     plasmid #752 cut with AgeI."
     misc_feature    4267..4960
                     /label=mCherry
                     /note="mCherry"
     CDS             4993..5466
                     /codon_start=1
                     /label=eDHFR
                     /note="residues 2-159"
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             5467..5495
                     /codon_start=1
                     /label=myc
                     /note="myc"
                     /translation="EQKLISEED"
     misc_feature    5515..6063
                     /label=globin 3'UTR
     misc_feature    6086..6119
                     /label=LoxP
     misc_feature    6713..7246
                     /label=ORI
     misc_feature    complement(7472..8332)
                     /label=Bla (AmpR)
     misc_feature    complement(8333..8431)
                     /label=Bla promoter
ORIGIN
        1 aagcttaata aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
       61 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
      121 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
      181 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
      241 gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca
      301 ttcgagaata aataacttcg tataaagtat cctatacgaa gttatcacca tgaccgagta
      361 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
      421 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
      481 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
      541 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
      601 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
      661 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
      721 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
      781 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
      841 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
      901 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
      961 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat ggctccgacc gaagccgacc
     1021 cgggcggccc cgccgacccc gcacccgccc ccgaggccca ccgactctag agctcgctga
     1081 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1141 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1201 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     1261 ggggaggatt gggaagacaa tagcactagt tattaatagt aatcaattac ggggtcatta
     1321 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     1381 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     1441 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     1501 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     1561 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1621 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     1681 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     1741 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc
     1801 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1861 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1921 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1981 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     2041 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     2101 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     2161 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     2221 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     2281 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     2341 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     2401 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     2461 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     2521 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     2581 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     2641 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     2701 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     2761 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     2821 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2881 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2941 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     3001 ctcgaccacc ggcgcgccac cggcaccatg agctcttctg caaaccctgt gaactcagtt
     3061 cggaggaaat catgtcttgt gaaggaagtg gaaaaaatga agaacaagcg agaagagaag
     3121 aaggcccaga actctgaaat gagaatgaag agagctcagg agtatgacag tagttttcca
     3181 aactgggaat ttgcccgaat gattaaagaa tttcgggcta ctttggaatg tcatccactt
     3241 actatgactg atcctatcga agagcacaga atatgtgtct gtgttaggaa acgcccactg
     3301 aataagcaag aattggccaa gaaagaaatt gatgtgattt ccattcctag caagtgtctc
     3361 ctcttggtac atgaacccaa gttgaaagtg gacttaacaa agtatctgga gaaccaagca
     3421 ttctgctttg actttgcatt tgatgaaaca gcttcgaatg aagttgtcta caggttcaca
     3481 gcaaggccac tggtacagac aatctttgaa ggtggaaaag caacttgttt tgcatatggc
     3541 cagacaggaa gtggcaagac acatactatg ggcggagacc tctctgggaa agcccagaat
     3601 gcatccaaag ggatctatgc catggcctcc cgggacgtct tcctcctgaa gaatcaaccc
     3661 tgctaccgga agttgggcct ggaagtctat gtgacattct tcgagatcta caatgggaag
     3721 ctgtttgacc tgctcaacaa gaaggccaag ctgcgcgtgc tggaggacgg caagcaacag
     3781 gtgcaagtgg tggggctgca ggagcatctg gttaactctg ctgatgatgt catcaagatg
     3841 atcgacatgg gcagcgcctg cagaacctct gggcagacat ttgccaactc caattcctcc
     3901 cgctcccacg cgtgcttcca aattattctt cgagctaaag ggagaatgca tggcaagttc
     3961 tctttggtag atctggcagg gaatgagcga ggcgcggaca cttccagtgc tgaccggcag
     4021 acccgcatgg agggcgcaga aatcaacaag agtctcttag ccctgaagga gtgcatcagg
     4081 gccctgggac agaacaaggc tcacaccccg ttccgtgaga gcaagctgac acaggtgctg
     4141 agggactcct tcattgggga gaactctagg acttgcatga ttgccacgat ctcaccaggc
     4201 ataagctcct gtgaatatac tttaaacacc ctgagatatg cagacggcga aagctcgccg
     4261 gtcaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     4321 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     4381 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     4441 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     4501 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     4561 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     4621 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     4681 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     4741 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     4801 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     4861 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     4921 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta taaggccggc
     4981 ctggagtcta gaatcagtct gattgcggcg ttagcggtag atcgcgttat cggcatggaa
     5041 aacgccatgc cgtggaacct gcctgccgat ctcgcctggt ttaaacgcaa caccttaaat
     5101 aaacccgtga ttatgggccg ccatacctgg gaatcaatcg gtcgtccgtt gccaggacgc
     5161 aaaaatatta tcctcagcag tcaaccgggt acggacgatc gcgtaacgtg ggtgaagtcg
     5221 gtggatgaag ccatcgcggc gtgtggtgac gtaccagaaa tcatggtgat tggcggcggt
     5281 cgcgtttatg aacagttctt gccaaaagcg caaaaactgt atctgacgca tatcgacgca
     5341 gaagtggaag gcgacaccca tttcccggat tacgagccgg atgactggga atcggtattc
     5401 agcgaattcc acgatgctga tgcgcagaac tctcacagct attgctttga gattctggag
     5461 cggcgggaac aaaaactcat ctcagaagag gatctttaat taagcgctcg aggcggccgc
     5521 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     5581 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     5641 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     5701 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     5761 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     5821 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     5881 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     5941 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     6001 tctcctgact actcccagtc atagctgtcc ctcttctctt atgaagatcc ctcgacctga
     6061 gcgtcgagct agcatatgga tccatataac ttcgtataat gtatgctata cgaagttatt
     6121 acgataccgt caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6181 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6241 tcaatgtatc ttaggtaccc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
     6301 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6361 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6421 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6481 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6541 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6601 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6661 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6721 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6781 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6841 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6901 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6961 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7021 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7081 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7141 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7201 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7261 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7321 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7381 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7441 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7501 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7561 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7621 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7681 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7741 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7801 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7861 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7921 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7981 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8041 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8101 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8161 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8221 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8281 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8341 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8401 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgcc
//
