LOCUS       Exported                9446 bp ds-DNA     circular SYN 27-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 27, 2021 from SnapGene Viewer 5.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          7242..9446
                     /label=present in pSuper
                     /note="present in pSuper"
     misc_feature    1..311
                     /label=TKpA
     misc_feature    312..345
                     /label=Lox2271
     CDS             350..949
                     /codon_start=1
                     /label=Pur
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    complement(669..688)
                     /label=EMO926 (pur_seqdn1)
     misc_feature    1091..1285
                     /label=bGH_3'UTR
     misc_feature    1097..1116
                     /label=bGH_3'UTR_seq2
     misc_feature    1181..1186
                     /label=pA
     misc_feature    1285..3000
                     /label=CAG promoter
     primer_bind     1996..2015
                     /label=EMO674
     primer_bind     complement(2086..2105)
                     /label=EMO502
     misc_feature    3013..3028
                     /label=CenpB halo intermediate
     CDS             3029..4828
                     /codon_start=1
                     /label=full length CenpB
                     /translation="MGPKRRQLTFREKSRIIQEVEENPDLRKGEIARRFNIPPSTLSTI
                     LKNKRAILASERKYGVASTCRKTNKLSPYDKLEGLLIAWFQQIRAAGLPVKGIILKEKA
                     LRIAEELGMDDFTASNGWLDRFRRRHGVVSCSGVARARARNAAPRTPAAPASPAAVPSE
                     GSGGSTTGWRAREEQPPSVAEGYASQDVFSATETSLWYDFLPDQAAGLCGGDGRPRQAT
                     QRLSVLLCANADGSEKLPPLVAGKSAKPRAGQAGLPCDYTANSKGGVTTQALAKYLKAL
                     DTRMAAESRRVLLLAGRLAAQSLDTSGLRHVQLAFFPPGTVHPLERGVVQQVKGHYRQA
                     MLLKAMAALEGQDPSGLQLGLTEALHFVAAAWQAVEPSDIAACFREAGFGGGPNATITT
                     SLKSEGEEEEEEEEEEEEEEGEGEEEEEEGEEEEEEGGEGEELGEEEEVEEEGDVDSDE
                     EEEEDEESSSEGLEAEDWAQGVVEAGGSFGAYGAQEEAQCPTLHFLEGGEDSDSDSEEE
                     DDEEEDDEDEDDDDDEEDGDEVPVPSFGEAMAYFAMVKRYLTSFPIDDRVQSHILHLEH
                     DLVHVTRKNHARQAGVRGLGHQSG"
     misc_feature    4828..4833
                     /label=NheI
                     /note="NheI"
     CDS             4844..5560
                     /codon_start=1
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    complement(5572..5579)
                     /label=HaloTag N-terminal tag
                     /note="HaloTag N-terminal tag"
     misc_feature    5579..6466
                     /label=HaloTag N-terminal tag
                     /note="HaloTag N-terminal tag"
     misc_feature    6477..6487
                     /label=IRES PCR 398 399
     misc_feature    6477..6487
                     /label=238-168 PCR IRES InFusion insert
     misc_feature    6477..6487
                     /label=IRES_Puro InFusion fragment
     misc_feature    6503..7051
                     /label=globin 3'UTR
     misc_feature    6509..6816
                     /label=insert detected by sequencing CAG_miR_3U
                     /note="insert detected by sequencing CAG_miR_3U"
     misc_feature    7074..7107
                     /label=LoxP
     misc_feature    7701..8234
                     /label=ORI
     misc_feature    complement(8460..9320)
                     /label=Bla (AmpR)
     misc_feature    complement(9321..9419)
                     /label=Bla promoter
ORIGIN
        1 aagcttaata aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
       61 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
      121 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
      181 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
      241 gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca
      301 ttcgagaata aataacttcg tataaagtat cctatacgaa gttatcacca tgaccgagta
      361 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
      421 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
      481 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
      541 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
      601 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
      661 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
      721 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
      781 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
      841 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
      901 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
      961 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat ggctccgacc gaagccgacc
     1021 cgggcggccc cgccgacccc gcacccgccc ccgaggccca ccgactctag agctcgctga
     1081 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1141 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1201 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     1261 ggggaggatt gggaagacaa tagcactagt tattaatagt aatcaattac ggggtcatta
     1321 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     1381 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     1441 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     1501 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     1561 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1621 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     1681 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     1741 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc
     1801 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1861 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1921 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1981 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     2041 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     2101 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     2161 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     2221 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     2281 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     2341 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     2401 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     2461 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     2521 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     2581 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     2641 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     2701 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     2761 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     2821 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2881 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2941 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     3001 ctcgaccacc ggcgcgccac cggtcaccat gggccccaag aggcgacagc tgacgttccg
     3061 ggagaagtca cggatcatcc aggaggtgga ggagaatccg gacctgcgca agggcgagat
     3121 cgcgcggcgc ttcaacatcc cgccgtccac gctgagcacg atcctgaaga acaagcgcgc
     3181 catcctggcg tcggagcgca agtacggggt ggcctccacc tgccgcaaga ccaacaagct
     3241 gtctccctac gacaagctcg agggcttgct catcgcctgg ttccagcaga tccgcgccgc
     3301 cggcctgccg gtcaagggca tcatcctcaa ggagaaggcg ctgcgcatag ccgaggagct
     3361 gggcatggac gacttcaccg cctccaacgg ctggctggac cgcttccgcc ggcgccacgg
     3421 cgtggtgtcc tgcagcggcg tggcccgcgc ccgcgcgcga aacgctgccc cccgcacccc
     3481 ggcggcgcct gccagtccgg ccgcggtgcc ctcggagggc agtggcggga gcactactgg
     3541 ttggcgcgct cgggaggagc agccgccgtc ggtggccgag ggctacgcct cgcaggacgt
     3601 gttcagcgcc accgagacca gtctatggta cgacttcctg cccgaccagg ccgcggggct
     3661 gtgcggaggc gacggacggc cgcgtcaagc cacccagcgc ctgagcgtcc tgctatgcgc
     3721 caatgccgac ggcagcgaga agctgccccc gctggtggcc ggcaagtcgg ccaagccccg
     3781 cgcaggccaa gccggcctgc cctgcgacta caccgccaac tccaagggtg gtgtcaccac
     3841 ccaggccctg gccaagtact tgaaggcctt ggacacccga atggctgcag agtctcgccg
     3901 ggtcctgctg ttggccggcc gcttggctgc ccagtccttg gacacctcgg gcctgcggca
     3961 tgtgcagctg gccttcttcc ctcccggcac cgtgcatccg ctggagaggg gagtggtcca
     4021 gcaggtgaag ggccactacc gccaggccat gctgctcaag gccatggccg cgctagaggg
     4081 ccaggatccc tcaggcctgc agctgggtct cacggaggcc ctgcactttg tggctgccgc
     4141 ctggcaggca gtggagcctt cggacatagc cgcctgcttt cgtgaggctg gctttggggg
     4201 tggccctaat gccaccatca ccacttccct caagagtgag ggagaggaag aggaggagga
     4261 ggaggaagaa gaggaggagg aagagggtga aggagaggaa gaggaggagg aaggggagga
     4321 ggaggaggag gaaggggggg aaggagagga attgggggag gaagaggagg tggaggagga
     4381 gggtgatgtt gatagtgatg aagaagagga ggaagatgag gagagctcct cggagggctt
     4441 ggaggctgag gactgggccc agggagtagt ggaggccggt ggcagcttcg gggcttatgg
     4501 tgcccaggag gaagcccagt gccctactct gcatttcctg gaaggtgggg aggactctga
     4561 ttcagacagt gaggaagagg acgatgagga agaggatgat gaagatgaag acgacgatga
     4621 tgatgaggag gatggtgatg aggtgcctgt acccagcttt ggggaggcca tggcttactt
     4681 tgccatggtc aagaggtacc tgacctcctt ccccattgat gaccgcgtgc agagccacat
     4741 cctccacttg gaacacgatc tggttcatgt gaccaggaag aaccacgcca ggcaggcggg
     4801 agttcgaggt cttggacatc aaagcgggct agcagtcgcc accatggtga gcaagggcga
     4861 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4921 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4981 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     5041 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     5101 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     5161 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     5221 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     5281 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     5341 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     5401 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     5461 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     5521 cgccgccggg atcactctcg gcatggacga gctgtacaag ggtggtgaaa cgcgtggtgc
     5581 agaaatcggt actggctttc cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat
     5641 gcactacgtc gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc acggtaaccc
     5701 gacctcctcc tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc atcgctgcat
     5761 tgctccagac ctgatcggta tgggcaaatc cgacaaacca gacctgggtt atttcttcga
     5821 cgaccacgtc cgcttcatgg atgccttcat cgaagccctg ggtctggaag aggtcgtcct
     5881 ggtcattcac gactggggct ccgctctggg tttccactgg gccaagcgca atccagagcg
     5941 cgtcaaaggt attgcattta tggagttcat ccgccctatc ccgacctggg acgaatggcc
     6001 agaatttgcc cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc gcaagctgat
     6061 catcgatcag aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc gcccgctgac
     6121 tgaagtcgag atggaccatt accgcgagcc gttcctgaat cctgttgacc gcgagccact
     6181 gtggcgcttc ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg tcgcgctggt
     6241 cgaagaatac atggactggc tgcaccagtc ccctgtcccg aagctgctgt tctggggcac
     6301 cccaggcgtt ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc tgcctaactg
     6361 caaggctgtg gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc cggacctgat
     6421 cggcagcgag atcgcgcgct ggctgtcgac gctcgagatt tccggctagc cggtctgcag
     6481 cctaggctta agcgctcgag gcggccgcaa ttcactcctc aggtgcaggc tgcctatcag
     6541 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     6601 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     6661 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     6721 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     6781 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     6841 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     6901 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     6961 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     7021 cttctcttat gaagatccct cgacctgagc gtcgagctag catatggatc catataactt
     7081 cgtataatgt atgctatacg aagttattac gataccgtca acttgtttat tgcagcttat
     7141 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7201 cattctagtt gtggtttgtc caaactcatc aatgtatctt aggtacccag cttttgttcc
     7261 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     7321 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7381 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7441 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7501 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7561 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7621 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7681 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7741 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7801 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7861 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7921 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7981 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8041 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8101 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8161 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8221 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8281 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8341 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8401 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8461 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8521 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8581 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8641 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8701 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8761 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8821 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8881 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8941 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9001 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9061 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9121 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9181 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9241 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9301 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9361 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9421 ccgcgcacat ttccccgaaa agtgcc
//
