LOCUS       Exported               10122 bp ds-DNA     circular SYN 27-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 27, 2021 from SnapGene Viewer 5.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..551
                     /label=CAG_promoter
                     /label=CAG_promoter(1)
     misc_feature    573..575
                     /label=START
                     /label=START(1)
     misc_feature    576..1463
                     /label=HaloTag7v2
     misc_feature    1491..2378
                     /label=HaloTag7v3
     misc_feature    2415..2417
                     /label=START
     misc_feature    2418..3305
                     /label=HaloTag
     misc_feature    3321..4027
                     /label=EGFP
     misc_feature    4028..4034
                     /label=EGFP
                     /label=EGFP(1)
     misc_feature    4056..4724
                     /label=hSpc25
     misc_feature    4725..4727
                     /label=STOP
     misc_recomb     complement(5301..5334)
                     /label=LoxP
     primer_bind     complement(5485..5504)
                     /label=T3
     primer_bind     complement(5485..5504)
                     /label=T3_promoter_primer_UPenn_seq
     primer_bind     complement(5522..5542)
                     /label=M13-rev
     primer_bind     complement(5525..5542)
                     /label=M13_Reverse_(-26)_UPenn_seq
     primer_bind     complement(5539..5561)
                     /label=M13_pUC_Reverse_UPenn_seq
     misc_binding    complement(5548..5570)
                     /label=LacO
     rep_origin      complement(5910..6592)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6690..7349)
                     /label=AmpR
     misc_recomb     complement(7985..8018)
                     /label=Lox2272
     CDS             8023..8571
                     /label=PurR
     primer_bind     complement(8758..8775)
                     /label=BGH_Reverse_UPenn_seq
     promoter        8958..10122
                     /label=CAG_promoter
ORIGIN
        1 cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg
       61 tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg
      121 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
      181 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
      241 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
      301 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
      361 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
      421 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
      481 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
      541 gcaaagaatt gctcgaccac cggcgcgcca caatggcaga gatcggtact ggcttcccat
      601 tcgatcccca ttacgtggaa gtcctgggcg aacgcatgca ctatgtcgat gttggtccgc
      661 gcgacggcac ccctgtgctg ttcctgcatg gtaacccgac ctcctcctat gtgtggcgca
      721 acattatccc gcacgttgca ccgacccatc gctgtattgc tccagatctg atcggtatgg
      781 gcaagtccga caagccagac ctgggttact tctttgacga tcacgtccgc tttatggatg
      841 cctttatcga ggccctgggt ctggaagaag tcgtcctggt cattcatgac tggggctccg
      901 ctctgggttt tcactgggcc aaacgcaatc cagaacgcgt caaaggtatc gcatttatgg
      961 aattcattcg ccctatcccg acctgggacg aatggccaga gtttgcccgc gaaaccttcc
     1021 aggcctttcg caccaccgac gtcggccgca aactgatcat tgatcagaat gtttttattg
     1081 agggtacgct gccgatgggt gtcgtccgcc cgctgactga ggtcgagatg gatcattatc
     1141 gcgagccgtt tctgaatcct gttgatcgcg agccactgtg gcgctttcca aacgaactgc
     1201 caatcgccgg tgaaccagcg aacattgtcg cgctggtcga agagtacatg gattggctgc
     1261 accagtcccc tgtcccgaaa ctgctgttct ggggcacccc aggcgttctg attccaccgg
     1321 ccgaagccgc tcgcctggcc aagagcctgc ctaactgcaa agctgtggac attggcccgg
     1381 gtctgaatct gctgcaagag gacaatccgg acctgattgg cagcgagatt gcgcgctggc
     1441 tgtcgacgct cgagatctcc ggcgcttcga attctgcagt cgacggtacc gcagaaattg
     1501 gtactggctt tccatttgac ccccactatg tggaggtcct gggcgagcgc atgcattacg
     1561 tcgacgttgg tccgcgcgat ggcacccctg tgctgtttct gcacggtaat ccgacctcct
     1621 cctacgtgtg gcgcaatatc attccgcatg ttgcaccgac ccaccgctgc atcgctccag
     1681 acctgattgg tatgggcaaa tccgataaac cagatctggg ttattttttc gatgaccatg
     1741 tccgcttcat ggacgccttc attgaagccc tgggtctgga ggaggtcgtc ctggtcatcc
     1801 acgattgggg ctccgctctg ggtttccatt gggccaagcg caacccagag cgcgtcaagg
     1861 gtattgcatt catggagttt atccgcccta ttccgacctg ggacgagtgg ccagaattcg
     1921 cccgcgagac ctttcaggcc ttccgcacca ccgatgtcgg ccgcaagctg attatcgacc
     1981 agaacgtttt catcgaaggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg
     2041 aaatggacca ctaccgcgaa ccgttcctga accctgttga ccgcgaacca ctgtggcgct
     2101 tcccaaatga gctgccaatt gccggtgagc cagcgaatat cgtcgcgctg gtcgaggaat
     2161 atatggactg gctgcatcag tcccctgtcc cgaagctgct gttttggggc accccaggcg
     2221 ttctgatccc accggccgag gccgctcgcc tggccaaaag cctgcctaat tgcaaggctg
     2281 tggatatcgg cccgggtctg aacctgctgc aagaagataa cccggatctg atcggcagcg
     2341 aaatcgcgcg ctggctgtcg acgctcgaaa tttccggcag tgctggtagt gctggtgaat
     2401 tgatgcgcgc caccatggca gaaatcggta ctggctttcc attcgacccc cattatgtgg
     2461 aagtcctggg cgagcgcatg cactacgtcg atgttggtcc gcgcgatggc acccctgtgc
     2521 tgttcctgca cggtaacccg acctcctcct acgtgtggcg caacatcatc ccgcatgttg
     2581 caccgaccca tcgctgcatt gctccagacc tgatcggtat gggcaaatcc gacaaaccag
     2641 acctgggtta tttcttcgac gaccacgtcc gcttcatgga tgccttcatc gaagccctgg
     2701 gtctggaaga ggtcgtcctg gtcattcacg actggggctc cgctctgggt ttccactggg
     2761 ccaagcgcaa tccagagcgc gtcaaaggta ttgcatttat ggagttcatc cgccctatcc
     2821 cgacctggga cgaatggcca gaatttgccc gcgagacctt ccaggccttc cgcaccaccg
     2881 acgtcggccg caagctgatc atcgatcaga acgtttttat cgagggtacg ctgccgatgg
     2941 gtgtcgtccg cccgctgact gaagtcgaga tggaccatta ccgcgagccg ttcctgaatc
     3001 ctgttgaccg cgagccactg tggcgcttcc caaacgagct gccaatcgcc ggtgagccag
     3061 cgaacatcgt cgcgctggtc gaagaataca tggactggct gcaccagtcc cctgtcccga
     3121 agctgctgtt ctggggcacc ccaggcgttc tgatcccacc ggccgaagcc gctcgcctgg
     3181 ccaaaagcct gcctaactgc aaggctgtgg acatcggccc gggtctgaat ctgctgcaag
     3241 aagacaaccc ggacctgatc ggcagcgaga tcgcgcgctg gctgtcgacg ctcgagattt
     3301 ccggttacgt agccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3361 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     3421 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     3481 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     3541 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     3601 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     3661 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     3721 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     3781 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     3841 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     3901 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     3961 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4021 acgagctgta caagtccgga ctcagatccc atatggtaga ggacgaactg gcacttttcg
     4081 ataaaagcat aaatgaattt tggaataaat tcaaaagtac ggacacctcc tgtcagatgg
     4141 cgggactaag agatacctac aaggattcca tcaaagcatt tgcagaaaag ctgtctgtga
     4201 aattaaagga agaagaacga atggttgaga tgtttctgga atatcaaaat cagatcagca
     4261 ggcaaaataa gctcattcaa gaaaaaaagg ataacttgtt aaaattgatt gctgaagtaa
     4321 aaggcaaaaa gcaggaattg gaagtactga ctgcaaatat ccaggatctt aaggaagaat
     4381 attctaggaa gaaggaaact atttctactg ctaataaagc gaatgcagag aggttgaaaa
     4441 ggctgcagaa atctgcagac ttgtataaag atcgacttgg actagaaatt cgaaaaattt
     4501 atggtgagaa attgcagttt attttcacta atattgaccc taagaatcct gagagcccat
     4561 ttatgttttc cttacatctc aatgaagcaa gggactatga agtgtcagat agtgcccctc
     4621 atcttgaggg cctagcagaa tttcaagaga atgtaaggaa gaccaacaat ttttcagctt
     4681 ttcttgccaa tgttcggaaa gcttttactg ccacggttta taattaagcg gccgcaattc
     4741 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
     4801 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
     4861 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     4921 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     4981 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
     5041 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
     5101 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
     5161 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
     5221 tgactactcc cagtcatagc tgtccctctt ctcttatgaa gatccctcga cctgagcgtc
     5281 gagctagcat atggatccat ataacttcgt ataatgtatg ctatacgaag ttattacgat
     5341 accgtcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5401 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5461 gtatcttagg tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     5521 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5581 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5641 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5701 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5761 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5821 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5881 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5941 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6001 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6061 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6121 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6181 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6241 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6301 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6361 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6421 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6481 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6661 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6721 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6781 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6841 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6901 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6961 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7021 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7081 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7141 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7201 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7261 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7321 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7381 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7441 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7501 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7561 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7621 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccaagctta
     7681 ataaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     7741 gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     7801 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     7861 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     7921 cggcaggccc tgccatagcc actggccccg tgggttaggg acggggtccc ccattcgaga
     7981 ataaataact tcgtataaag tatcctatac gaagttatca ccatgaccga gtacaagccc
     8041 acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg
     8101 ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc
     8161 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     8221 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     8281 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     8341 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     8401 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     8461 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     8521 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     8581 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     8641 cagcgcccga ccgaaaggag cgcacgaccc catggctccg accgaagccg acccgggcgg
     8701 ccccgccgac cccgcacccg cccccgaggc ccaccgactc tagagctcgc tgatcagcct
     8761 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     8821 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     8881 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     8941 attgggaaga caatagcact agttattaat agtaatcaat tacggggtca ttagttcata
     9001 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     9061 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     9121 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     9181 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     9241 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     9301 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
     9361 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     9421 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
     9481 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
     9541 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
     9601 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
     9661 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     9721 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
     9781 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
     9841 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
     9901 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     9961 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
    10021 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
    10081 acccccctcc ccgagttgct gagcacggcc cggcttcggg tg
//
