LOCUS       pRedCm                  7555 bp    DNA     circular     30-AUG-2021
DEFINITION  pQE-30.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14022182|
COMMENT     VNTDBDATE|-14022182|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRedCm|
COMMENT     VNTAUTHORNAME|Dmitrii|
COMMENT     VNTOAUTHORNAME|user|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (CMolPar 0 0 1 3117 3136 1 7555 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT            (CStringList "/gene=\"Ampicillin\"") 1 1 1 1 
COMMENT            "complement(4574..5434)") 
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COMMENT        #2=(CFSignal (CObList) "RNAII" 33 0 1 3815 4367 0 (CStringList) 
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COMMENT        #3=(CFSignal (CObList) "RNAI" 33 0 0 4257 4366 0 (CStringList) 
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COMMENT        #4=(CFSignal (CObList) "TrrnB" 43 0 0 2368 2624 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2368..2624") 
COMMENT        #5=(CFSignal (CObList) "bet" 4 0 0 528 1313 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "528..1313") 
COMMENT        #6=(CFSignal (CObList) "exo" 4 0 0 1310 1990 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1310..1990") 
COMMENT        #7=(CFSignal (CObList) "tL3" 43 0 0 1980 2249 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1980..2249") 
COMMENT        #8=(CFSignal (CObList) "rhaR" 4 0 1 5687 6535 0 (CStringList) 
COMMENT            (CStringList "/gene=\"rhaR\"" "/locus_tag=\"b3906\"" 
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COMMENT             "/function=\"regulator; Degradation of small molecules:" 
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COMMENT             "/GO_function=\"GO:0016563 - transcription activator" "activity\"" 
COMMENT             "/GO_process=\"GO:0016052 - carbohydrate catabolic process;" 
COMMENT             "GO:0006350 - transcription\"" "/codon_start=1" "/transl_table=11" 
COMMENT             "/product=\"DNA-binding transcriptional activator for rhaSR," 
COMMENT             "L-rhamnose-binding\"" "/protein_id=\"NP_418342.2\"" 
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COMMENT             "/db_xref=\"ECOCYC:EG10843\"" "/db_xref=\"EcoGene:EG10843\"" 
COMMENT             "/db_xref=\"GeneID:948397\"") 1 1 1 1 "complement(6609..7445)") 
COMMENT        #10=(CFSignal (CObList) "PrhaBAD" 30 0 0 1 162 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "t(0) terminator" 43 0 0 5565 5659 0 
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COMMENT        #12=(CFSignal (CObList) "gamS" 4 0 0 226 522 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "cat" 4 0 0 2741 3400 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "OP L2" 21 0 0 2654 2670 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "OP L1" 21 0 0 2678 2694 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2678..2694") 
COMMENT        #17=(CFSignal (CObList) "PL" 30 0 0 2668 2696 0 (CStringList) 
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COMMENT                          "Created: 08/30/21 01:17" 1) 
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COMMENT                          "Last Modified: 08/30/21 01:17" 1) 
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COMMENT                          "length: 7555 bp" 1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/other+sequences;+artificial+sequences;+vectors.\">other sequences; artificial sequences; vectors.(other sequences; artificial sequences; vectors.)</a>" 
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COMMENT                          "Associated Genes:  Ampicillin;  rhaR;  ECK3899; JW3877; rhaC1;  rhaS;  ECK3898; JW3876; rhaC2" 
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FEATURES             Location/Qualifiers
     CDS             complement(4574..5434)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=bla
                     /note="ORF frame 2"
     gene            complement(4574..5434)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        3435..3514
                     /vntifkey="30"
                     /label=T5\promoter/lac\operator\element
     rep_origin      complement(3815..4367)
                     /vntifkey="33"
                     /label=RNAII
     rep_origin      4257..4366
                     /vntifkey="33"
                     /label=RNAI
     terminator      2368..2624
                     /vntifkey="43"
                     /label=TrrnB
     CDS             528..1313
                     /vntifkey="4"
                     /label=bet
     CDS             1310..1990
                     /vntifkey="4"
                     /label=exo
     terminator      1980..2249
                     /vntifkey="43"
                     /label=tL3
     CDS             complement(5687..6535)
                     /gene="rhaR"
                     /locus_tag="b3906"
                     /gene_synonym="ECK3899; JW3877; rhaC1"
                     /function="regulator; Degradation of small molecules:
                     Carbon compounds"
                     /GO_component="GO:0005737 - cytoplasm"
                     /GO_function="GO:0016563 - transcription activator
                     activity"
                     /GO_process="GO:0016052 - carbohydrate catabolic process;
                     GO:0006350 - transcription"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator for rhaSR,
                     L-rhamnose-binding"
                     /protein_id="NP_418342.2"
                     /db_xref="GI:171701684"
                     /db_xref="ASAP:ABE-0012747"
                     /db_xref="UniProtKB/Swiss-Prot:P09378"
                     /db_xref="ECOCYC:EG10842"
                     /db_xref="EcoGene:EG10842"
                     /db_xref="GeneID:948396"
                     /vntifkey="4"
                     /label=rhaR
     CDS             complement(6609..7445)
                     /gene="rhaS"
                     /locus_tag="b3905"
                     /gene_synonym="ECK3898; JW3876; rhaC2"
                     /function="regulator; Degradation of small molecules:
                     Carbon compounds"
                     /GO_component="GO:0005737 - cytoplasm"
                     /GO_function="GO:0016563 - transcription activator
                     activity"
                     /GO_process="GO:0016052 - carbohydrate catabolic process;
                     GO:0006350 - transcription"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator for rhaBAD
                     and rhaT, L-rhamnose-binding"
                     /protein_id="NP_418341.1"
                     /db_xref="GI:16131745"
                     /db_xref="ASAP:ABE-0012745"
                     /db_xref="UniProtKB/Swiss-Prot:P09377"
                     /db_xref="ECOCYC:EG10843"
                     /db_xref="EcoGene:EG10843"
                     /db_xref="GeneID:948397"
                     /vntifkey="4"
                     /label=rhaS
     promoter        1..162
                     /vntifkey="30"
                     /label=PrhaBAD
     terminator      5565..5659
                     /vntifkey="43"
                     /label=t(0)\terminator
     CDS             226..522
                     /vntifkey="4"
                     /label=gamS
     CDS             2741..3400
                     /vntifkey="4"
                     /label=cat
     promoter        2668..2696
                     /vntifkey="30"
                     /label=PL
     misc_feature    2634..2650
                     /vntifkey="21"
                     /label=OP\L3
     misc_feature    2654..2670
                     /vntifkey="21"
                     /label=OP\L2
     misc_feature    2678..2694
                     /vntifkey="21"
                     /label=OP\L1
BASE COUNT     1993 a      1871 c      1877 g      1814 t 
ORIGIN
        1 gcgtgaattt tcaggaaatg cggtgagcat cacatcacca caattcagca aattgtgaac 
       61 atcatcacgt tcatctttcc ctggttgcca atggcccatt ttcctgtcag taacgagaag 
      121 gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa tctctagact aatcagcccg 
      181 gcatttcgcg ggcgatattt tcacagctat ttcaggagtt cagccatgaa cgcttattac 
      241 attcaggatc gtcttgaggc tcagagctgg gcgcgtcact accagcagct cgcccgtgaa 
      301 gagaaagagg cagaactggc agacgacatg gaaaaaggcc tgccccagca cctgtttgaa 
      361 tcgctatgca tcgatcattt gcaacgccac ggggccagca aaaaatccat tacccgtgcg 
      421 tttgatgacg atgttgagtt tcaggagcgc atggcagaac acatccggta catggttgaa 
      481 accattgctc accaccaggt tgatattgat tcagaggtat aaaacgaatg agtactgcac 
      541 tcgcaacgct ggctgggaag ctggctgaac gtgtcggcat ggattctgtc gacccacagg 
      601 aactgatcac cactcttcgc cagacggcat ttaaaggtga tgccagcgat gcgcagttca 
      661 tcgcattact gatcgttgcc aaccagtacg gccttaatcc gtggacgaaa gaaatttacg 
      721 cctttcctga taagcagaat ggcatcgttc cggtggtggg cgttgatggc tggtcccgca 
      781 tcatcaatga aaaccagcag tttgatggca tggactttga gcaggacaat gaatcctgta 
      841 catgccggat ttaccgcaag gaccgtaatc atccgatctg cgttaccgaa tggatggatg 
      901 aatgccgccg cgaaccattc aaaactcgcg aaggcagaga aatcacgggg ccgtggcagt 
      961 cgcatcccaa acggatgtta cgtcataaag ccatgattca gtgtgcccgt ctggccttcg 
     1021 gatttgctgg tatctatgac aaggatgaag ccgagcgcat tgtcgaaaat actgcataca 
     1081 ctgcagaacg tcagccggaa cgcgacatca ctccggttaa cgatgaaacc atgcaggaga 
     1141 ttaacactct gctgatcgcc ctggataaaa catgggatga cgacttattg ccgctctgtt 
     1201 cccagatatt tcgccgcgac attcgtgcat cgtcagaact gacacaggcc gaagcagtaa 
     1261 aagctcttgg attcctgaaa cagaaagccg cagagcagaa ggtggcagca tgacaccgga 
     1321 cattatcctg cagcgtaccg ggatcgatgt gagagctgtc gaacaggggg atgatgcgtg 
     1381 gcacaaatta cggctcggcg tcatcaccgc ttcagaagtt cacaacgtga tagcaaaacc 
     1441 ccgctccgga aagaagtggc ctgacatgaa aatgtcctac ttccacaccc tgcttgctga 
     1501 ggtttgcacc ggtgtggctc cggaagttaa cgctaaagca ctggcctggg gaaaacagta 
     1561 cgagaacgac gccagaaccc tgtttgaatt cacttccggc gtgaatgtta ctgaatcccc 
     1621 gatcatctat cgcgacgaaa gtatgcgtac cgcctgctct cccgatggtt tatgcagtga 
     1681 cggcaacggc cttgaactga aatgcccgtt tacctcccgg gatttcatga agttccggct 
     1741 cggtggtttc gaggccataa agtcagctta catggcccag gtgcagtaca gcatgtgggt 
     1801 gacgcgaaaa aatgcctggt actttgccaa ctatgacccg cgtatgaagc gtgaaggcct 
     1861 gcattatgtc gtgattgagc gggatgaaaa gtacatggcg agttttgacg agatcgtgcc 
     1921 ggagttcatc gaaaaaatgg acgaggcact ggctgaaatt ggttttgtat ttggggagca 
     1981 atggcgatga cgcatcctca cgataatatc cgggtaggcg caatcacttt cgtctactcc 
     2041 gttacaaagc gaggctgggt atttcccggc ctttctgtta tccgaaatcc actgaaagca 
     2101 cagcggctgg ctgaggagat aaataataaa cgaggggctg tatgcacaaa gcatcttctg 
     2161 ttgagttaag aacgagtatc gagatggcac atagccttgc tcaaattgga atcaggtttg 
     2221 tgccaatacc agtagaaaca gacgaagaat tctaggctgg agaatctcga tccggactcc 
     2281 ctgacgccac gccaggcgct ggagtggatc tatcggctga aaagtctggt gtaaggatcg 
     2341 tggcagacaa gcccgtcagg gcgcgtccat gcgagagtag ggaactgcca ggcatcaaat 
     2401 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 
     2461 cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc 
     2521 cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc 
     2581 catcctgacg gatggccttt ttgcgtttct acaaactctt cctgccatga gataaccatc 
     2641 tgcggtgata aattatctct ggcggtgttg acctaaatac cactggcggt gatactgagc 
     2701 acatcagcag gcgcactgac caggagctaa ggaagctaaa atggagaaaa aaatcactgg 
     2761 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc 
     2821 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac 
     2881 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat 
     2941 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag 
     3001 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag 
     3061 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta 
     3121 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc 
     3181 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt 
     3241 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct 
     3301 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga 
     3361 attacaacag tactgcgatg agtggcaggg cggggcgtaa catggccgct aggccctttc 
     3421 gtcttcacct cgagaaatca taaaaaattt atttgctttg tgagcggata acaattataa 
     3481 tagattcaat tgtgagcgga taacaatttc acacagaatt cattaaagag gagaaattaa 
     3541 ctatgagagg atcgcatcac catcaccatc acggatctat gcggtgtgaa ataccgcaca 
     3601 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc 
     3661 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 
     3721 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 
     3781 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 
     3841 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 
     3901 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 
     3961 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 
     4021 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 
     4081 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 
     4141 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 
     4201 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 
     4261 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 
     4321 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 
     4381 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 
     4441 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 
     4501 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 
     4561 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 
     4621 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 
     4681 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 
     4741 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 
     4801 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 
     4861 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 
     4921 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 
     4981 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 
     5041 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 
     5101 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 
     5161 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 
     5221 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 
     5281 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 
     5341 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 
     5401 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 
     5461 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 
     5521 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgactcc tgttgataga 
     5581 tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc 
     5641 gttttttatt ggtgagaatg acgtccgtcg gcatcggcat ggcgaattaa tctttctgcg 
     5701 aattgagatg acgccactgg ctgggcgtca tcccggtttc ccgggtaaac accaccgaaa 
     5761 aatagttact atcttcaaag ccacattcgg tcgaaatatc actgattaac aggcggctat 
     5821 gctggagaag atattgcgca tgacacactc tgacctgtcg cagatattga ttgatggtca 
     5881 ttccagtctg ctggcgaaat tgctgacgca aaacgcgctc actgcacgat gcctcatcac 
     5941 aaaatttatc cagcgcaaag ggacttttca ggctagccgc cagccgggta atcagcttat 
     6001 ccagcaacgt ttcgctggat gttggcggca acgaatcact ggtgtaacga tggcgattca 
     6061 gcaacatcac caactgcccg aacagcaact cagccatttc gttagcaaac ggcacatgct 
     6121 gactactttc atgctcaagc tgaccgataa cctgccgcgc ctgcgccatc cccatgctac 
     6181 ctaagcgcca gtgtggttgc cctgcgctgg cgttaaatcc cggaatcgcc ccctgccagt 
     6241 caagattcag cttcagacgc tccgggcaat aaataatatt ctgcaaaacc agatcgttaa 
     6301 cggaagcgta ggagtgttta tcgtcagcat gaatgtaaaa gagatcgcca cgggtaatgc 
     6361 gataagggcg atcgttgagt acatgcaggc cattaccgcg ccagacaatc accagctcac 
     6421 aaaaatcatg tgtatgttca gcaaagacat cttgcggata acggtcagcc acagcgactg 
     6481 cctgctggtc gctggcaaaa aaatcatctt tgagaagttt taactgatgc gccaccgtgg 
     6541 ctacctcggc cagagaacga agttgattat tcgcaatatg gcgtacaaat acgttgagaa 
     6601 gattcgcgtt attgcagaaa gccatcccgt ccctggcgaa tatcacgcgg tgaccagtta 
     6661 aactctcggc gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccaca gcgataggcg 
     6721 atgtcagtaa cgctggcctc gctgtggcgt agcagatgtc gggctttcat cagtcgcagg 
     6781 cggttcaggt atcgctgagg cgtcagtccc gtttgctgct taagctgccg atgtagcgta 
     6841 cgcagtgaaa gagaaaattg atccgccacg gcatcccaat tcacctcatc ggcaaaatgg 
     6901 tcctccagcc aggccagaag caagttgaga cgtgatgcgc tgttttccag gttctcctgc 
     6961 aaactgcttt tacgcagcaa gagcagtaat tgcataaaca agatctcgcg actggcggtc 
     7021 gagggtaaat cattttcccc ttcctgctgt tccatctgtg caaccagctg tcgcacctgc 
     7081 tgcaatacgc tgtggttaac gcgccagtga gacggatact gcccatccag ctcttgtggc 
     7141 agcaactgat tcagcccggc gagaaactga aatcgatccg gcgagcgata cagcacattg 
     7201 gtcagacaca gattatcggt atgttcatac agatgccgat catgatcgcg tacgaaacag 
     7261 accgtgccac cggtgatggt atagggctgc ccattaaaca catgaatacc cgtgccatgt 
     7321 tcgacaatca caatttcatg aaaatcatga tgatgttcag gaaaatccgc ctgcgggagc 
     7381 cggggttcta tcgccacgga cgcgttacca gacggaaaaa aatccacact atgtaatacg 
     7441 gtcatactgg cctcctgatg tcgtcaacac ggcgaaatag taatcacgag gtcaggttct 
     7501 taccttaaat tttcgacgga aaaccacgta aaaaacgtcg atttttcaag ataca 
//
