LOCUS       pTmpKmA1O4in            6244 bp    DNA     circular SYN 30-AUG-2021
DEFINITION  Cloning vector pMW118 gene for repA protein, beta-lactamase, complete and partial cds.
ACCESSION   AB005475
VERSION     AB005475.2  GI:67423788
SOURCE      Cloning vector pMW118.
  ORGANISM  Cloning vector pMW118
            other sequences; artificial sequences; vectors.
REFERENCE   1
  AUTHORS   Watahiki,M.
  TITLE     pMW118, low-copy number plasmid vector in Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Watahiki,M. and Hayashi,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-1997) Masanori Watahiki, Nippon Gene Co., Ltd.,
            Research & Development; Tonya-machi 1-29, Toyama, Toyama 930-0834,
            Japan (E-mail:masanori.watahiki@pref.toyama.lg.jp,
            Tel:81-764-51-6548, Fax:81-764-51-6547)
COMMENT     On Jun 9, 2005 this sequence version replaced gi:2257571.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14022179|
COMMENT     VNTDBDATE|-14022179|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTmpKmA1O4in|
COMMENT     VNTAUTHORNAME|Dmitrii|
COMMENT     VNTOAUTHORNAME|gen|
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COMMENT                          "DNA 'pTmpKmA1O4in'" 1) 
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COMMENT                          "Cloning vector pMW118 gene for repA protein, beta-lactamase, complete and partial cds." 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/AB005475\">AB005475</a>" 
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COMMENT                          "Sequence Source: Cloning vector pMW118." 1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/Cloning+vector+pMW118\">Cloning vector pMW118(Cloning vector pMW118)</a>" 
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FEATURES             Location/Qualifiers
     CDS             3781..4731
                     /codon_start=1
                     /transl_table=11
                     /product="repA protein"
                     /protein_id="BAA21463.2"
                     /db_xref="GI:67423789"
                     /vntifkey="4"
                     /label=repA
     CDS             complement(1964..2821)
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="BAA21464.1"
                     /db_xref="GI:2257573"
                     /vntifkey="4"
                     /label=bla
     CDS             complement(5484..6197)
                     /vntifkey="4"
                     /label=CI
     promoter        complement(6204..6232)
                     /vntifkey="30"
                     /label=PRM
     promoter        1..29
                     /vntifkey="30"
                     /label=PR
     CDS             63..221
                     /vntifkey="4"
                     /label=hok
     CDS             complement(232..1026)
                     /codon_start=1
                     /transl_table=11
                     /product="Tn5 neomycin phosphotransferase"
                     /protein_id="AAL02037.1"
                     /db_xref="GI:15554336"
                     /vntifkey="4"
                     /label=neo
     promoter        complement(1047..1085)
                     /vntifkey="30"
                     /label=Pcon
     primer          1121..1141
                     /vntifkey="27"
                     /label=M13\-46\R
     misc_feature    5271..5290
                     /vntifkey="21"
                     /label=M13\-46\F
     promoter        5333..5402
                     /vntifkey="30"
                     /label=PA1O4
     terminator      5409..5460
                     /vntifkey="43"
                     /label=T_L3S1P56
     -35_signal      5348..5353
                     /vntifkey="48"
                     /label=-35
     -10_signal      5371..5376
                     /vntifkey="47"
                     /label=-10
BASE COUNT     1793 a      1445 c      1428 g      1578 t 
ORIGIN
        1 ttgactattt tacctctggc ggtgataatg gttgcatgta ctttaaagag gagaaattaa 
       61 ctatgaaact accacgaagt tcccttgtct ggtgtgtgtt gatcgtgtgt ctcacactgt 
      121 tgatattcac ttatctgaca cgaaaatcgc tgtgcgagat tcgttacaga gacggacaca 
      181 gggaggtggc ggctttcatg gcttacgaat ccggtaagta gcaacctgga gttagaagaa 
      241 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag 
      301 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa 
      361 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa 
      421 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc 
      481 ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg 
      541 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg 
      601 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag 
      661 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag 
      721 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac 
      781 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc 
      841 gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc 
      901 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc 
      961 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc 
     1021 aatcatgcga aacgatcctc atcctgtatg gtaccattat aaccgccaag cttaaaatgt 
     1081 caacattttt aataccttta agtgatacca gatggcattg gctcacaatt ccacacaaca 
     1141 tgggtaccga gctcgaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 
     1201 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 
     1261 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 
     1321 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 
     1381 attgggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
     1441 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
     1501 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
     1561 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
     1621 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 
     1681 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
     1741 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
     1801 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
     1861 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 
     1921 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 
     1981 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 
     2041 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 
     2101 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 
     2161 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 
     2221 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag 
     2281 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 
     2341 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 
     2401 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 
     2461 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 
     2521 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac 
     2581 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 
     2641 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 
     2701 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 
     2761 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 
     2821 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 
     2881 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 
     2941 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 
     3001 gtatcacgag gccctttcgt cttcaagaat tgacagtaag acgggtaagc ctgttgatga 
     3061 taccgctgcc ttactgggtg cattagccag tctgaatgac ctgtcacggg ataatccgaa 
     3121 gtggtcagac tggaaaatca gagggcagga actgcagaac agcaaaaagt cagatagcac 
     3181 cacatagcag acccgccata aaacgccctg agaagcccgt gacgggcttt tcttgtatta 
     3241 tgggtagttt ccttgcatga atccataaaa ggcgcctgta gtgccattta cccccattca 
     3301 ctgccagagc cgtgagcgca gcgaactgaa tgtcacgaaa aagacagcga ctcaggtgcc 
     3361 tgatggtcgg agacaaaagg aatattcagc gatttgcccg agcttgcgag ggtgctactt 
     3421 aagcctttag ggttttaagg tctgttttgt agaggagcaa acagcgtttg cgacatcctt 
     3481 ttgtaatact gcggaactga ctaaagtagt gagttataca cagggctggg atctattctt 
     3541 tttatctttt tttattcttt ctttattcta taaattataa ccacttgaat ataaacaaaa 
     3601 aaaacacaca aaggtctagc ggaatttaca gagggtctag cagaatttac aagttttcca 
     3661 gcaaaggtct agcagaattt acagataccc acaactcaaa ggaaaaggac tagtaattat 
     3721 cattgactag cccatctcaa ttggtatagt gattaaaatc acctagacca attgagatgt 
     3781 atgtctgaat tagttgtttt caaagcaaat gaactagcga ttagtcgcta tgacttaacg 
     3841 gagcatgaaa ccaagctaat tttatgctgt gtggcactac tcaaccccac gattgaaaac 
     3901 cctacaagga aagaacggac ggtatcgttc acttataacc aatacgctca gatgatgaac 
     3961 atcagtaggg aaaatgctta tggtgtatta gctaaagcaa ccagagagct gatgacgaga 
     4021 actgtggaaa tcaggaatcc tttggttaaa ggctttgaga ttttccagtg gacaaactat 
     4081 gccaagttct caagcgaaaa attagaatta gtttttagtg aagagatatt gccttatctt 
     4141 ttccagttaa aaaaattcat aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa 
     4201 tactctatga ggatttatga gtggttatta aaagaactaa cacaaaagaa aactcacaag 
     4261 gcaaatatag agattagcct tgatgaattt aagttcatgt taatgcttga aaataactac 
     4321 catgagttta aaaggcttaa ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact 
     4381 tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac gttgattttc 
     4441 caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa ccagataaaa 
     4501 atgaatggtg acaaaatacc aacaaccatt acatcagatt cctacctaca taacggacta 
     4561 agaaaaacac tacacgatgc tttaactgca aaaattcagc tcaccagttt tgaggcaaaa 
     4621 tttttgagtg acatgcaaag taagcatgat ctcaatggtt cgttctcatg gctcacgcaa 
     4681 aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg aggttcttat 
     4741 ggctcttgta tctatcagtg aagcatcaag actaacaaac aaaagtagaa caactgttca 
     4801 ccgttacata tcaaagggaa aactgtccat atgcacagat gaaaacggtg taaaaaagat 
     4861 agatacatca gagcttttac gagtttttgg tgcattcaaa gctgttcacc atgaacagat 
     4921 cgacaatgta acagatgaac agcatgtaac acctaataga acaggtgaaa ccagtaaaac 
     4981 aaagcaacta gaacatgaaa ttgaacacct gagacaactt gttacagctc aacagtcaca 
     5041 catagacagc ctgaaacagg cgatgctgct tatcgaatca aagctgccga caacacggga 
     5101 gccagtgacg cctcccgtgg ggaaaaaatc atggcaattc tggaagaaat agcgccattc 
     5161 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 
     5221 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 
     5281 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt tttcgggatc ctttatcaaa 
     5341 aagagtgttg acttgtgagc ggataacaat gatacttaga ttcatcgaga gggacacggc 
     5401 gatctagatt ttcgaaaaaa ggcctcccaa atcggggggc cttttttatt gataacaaaa 
     5461 cgccgaccag aacaccttgc cgatcagcca aacgtctctt caggccactg actagcgata 
     5521 actttcccca caacggaaca actctcattg catgggatca ttgggtactg tgggtttagt 
     5581 ggttgtaaaa acacctgacc gctatccctg atcagtttct tgaaggtaaa ctcatcaccc 
     5641 ccaagtctgg ctatgcagaa atcacctggc tcaacagcct gctcagggtc aacgagaatt 
     5701 aacattccgt caggaaagct tggcttggag cctgttggtg cggtcatgga attaccttca 
     5761 acctcaagcc agaatgcaga atcactggct tttttggttg tgcttaccca tctctccgca 
     5821 tcacctttgg taaaggttct aagcttaggt gagaacatcc ctgcctgaac atgagaaaaa 
     5881 acagggtact catactcact tctaagtgac ggctgcatac taaccgcttc atacatctcg 
     5941 tagatttctc tggcgattga agggctaaat tcttcaacgc taactttgag aatttttgca 
     6001 agcaatgcgg cgttataagc atttaatgca ttgatgccat taaataaagc accaacgcct 
     6061 gactgcccca tccccatctt gtctgcgaca gattcctggg ataagccaag ttcatttttc 
     6121 tttttttcat aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg tgttaatggt 
     6181 ttcttttttg tgctcatacg ttaaatctat caccgcaagg gataaatatc taacaccgtg 
     6241 cgtg 
//
