LOCUS       Exported                5405 bp DNA     circular SYN 08-SEP-2021
DEFINITION  .
ACCESSION   VB181207-1020avc
VERSION     .
KEYWORDS    pAAV-SynI-CreOn-FlpOff-EGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 8, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="synthetic DNA construct"
                     /note="color: #ffffff"
     misc_feature    1..141
                     /label=5' ITR
                     /label=nonstandard type: ITR
                     /note="color: #fd3434; direction: RIGHT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows replication of the viral 
                     genomeand its packaging into virus."
                     /note="/description=AAV 5' inverted terminal repeat 
                     (functionalequivalent of wild-type 5' ITR)"
                     /note="/name=5' ITR"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=5f14ec1a645a407dab2725d71bd1c86a"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     promoter        169..637
                     /label=SYN1
                     /note="color: #46c6ef; direction: RIGHT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Tissue specificity: Brain. Cell 
                     typespecificity: Mature neurons."
                     /note="/description=[u'Human synapsin I promoter']"
                     /note="/full_name=Human synapsin I promoter"
                     /note="/name=SYN1"
                     /note="/official_designation=SYN1"
                     /note="/uuid=6f036b2e82244653b8e92867f598e473"
                     /note="/vntifkey=21"
                     /note="color: #ffffff"
     misc_feature    668..701
                     /label=lox2272
                     /note="color: #eda481; direction: RIGHT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Recognition site of Cre 
                     recombinase;can't work with other types of Lox site."
                     /note="/name=lox2272"
                     /note="/official_designation=Lox2272"
                     /note="/uuid=a19dd1e203df40eb846e5102df13ffb4"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    755..788
                     /label=loxP
                     /note="color: #da3bb5; direction: RIGHT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Recognition site of Cre 
                     recombinase."
                     /note="/name=loxP"
                     /note="/official_designation=LoxP"
                     /note="/uuid=b48e25a55648467f8c98b3f68faf613f"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    795..828
                     /label=FRT
                     /note="color: #e4b930; direction: RIGHT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=FRT"
                     /note="/official_designation=FRT"
                     /note="/uuid=6a7783e08a0e460dab0020bf416197b2"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    864..871
                     /label=NotI
                     /note="color: #7a00b5; direction: RIGHT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows linearization of vector."
                     /note="/name=NotI"
                     /note="/official_designation=NotI"
                     /note="/uuid=9f9f0cb60ddb47d7b6198deeaf89eaba"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    complement(872..1591)
                     /label=EGFP
                     /note="color: #05696d; direction: LEFT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Commonly used green 
                     fluorescentprotein; ranked high in brightness, 
                     photostability and pH stability among all fluorescent 
                     proteins."
                     /note="/full_name=EGFP reporter gene"
                     /note="/name=EGFP"
                     /note="/official_designation=EGFP"
                     /note="/uuid=14bbdeb46af6422aafbe7a45e8854f81"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    1592..1597
                     /label=Kozak
                     /note="color: #f54c6b; direction: RIGHT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/full_name=Kozak"
                     /note="/name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=ba4f837cfff042e38e7561244b24cfa6"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1598..1603
                     /label=BamHI
                     /note="color: #944603; direction: RIGHT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=BamHI"
                     /note="/official_designation=BamHI"
                     /note="/uuid=2e24d26a052f4a8f9539513b7d1f974d"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1634..1667
                     /label=FRT
                     /note="color: #ce0084; direction: RIGHT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=FRT"
                     /note="/official_designation=FRT"
                     /note="/uuid=6a7783e08a0e460dab0020bf416197b2"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    complement(1677..1710)
                     /label=lox2272
                     /note="color: #6d3ed8; direction: LEFT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Recognition site of Cre 
                     recombinase;can't work with other types of Lox site."
                     /note="/name=lox2272"
                     /note="/official_designation=Lox2272"
                     /note="/uuid=991e0551b2034abd9c3ad4e364260ba6"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(1761..1794)
                     /label=loxP
                     /note="color: #0375bb; direction: LEFT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Recognition site of Cre 
                     recombinase."
                     /note="/name=loxP"
                     /note="/official_designation=LoxP"
                     /note="/uuid=f4dc79ba31354e87bba6736ab88dbb23"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    1825..2422
                     /label=WPRE
                     /label=nonstandard type: Miscellaneous
                     /note="color: #a949ca; direction: RIGHT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/description=[u'Woodchuck hepatitis 
                     virusposttranscriptional regulatory element']"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/name=WPRE"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     polyA_signal    2453..2660
                     /label=BGH pA
                     /note="color: #946a00; direction: RIGHT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=[u'Bovine growth hormone 
                     polyadenylationsignal']"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/name=BGH pA"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4a09734ca6c745b3aadc757c1638b651"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    2668..2808
                     /label=3' ITR
                     /label=nonstandard type: ITR
                     /note="color: #512bbd; direction: RIGHT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows replication of the viral 
                     genomeand its packaging into virus."
                     /note="/description=AAV 3' inverted terminal repeat"
                     /note="/name=3' ITR"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=5b8101593b634e64aa9eef9434216f71"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    3725..4585
                     /label=Ampicillin
                     /label=nonstandard type: ORF
                     /note="color: #5cad08; direction: RIGHT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/description=[u'Ampicillin resistance gene']"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=Ampicillin"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=5bcc09480c0e4c07b95ddccd8242d769"
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     rep_origin      4756..5344
                     /label=pUC ori
                     /note="color: #d84e4e; direction: RIGHT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=[u'pUC origin of replication']"
                     /note="/full_name=pUC origin of replication"
                     /note="/name=pUC ori"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
                     /note="/vntifkey=21"
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttctagacaa ctttgtatag aaaagttgct gcagagggcc
      181 ctgcgtatga gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct
      241 gacgaccgac cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc
      301 cctatcagag agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc
      361 agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac
      421 tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg
      481 tcgcgtccgc gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg
      541 gcacgggcgc gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt
      601 gggcagcgga ggagtcgtgt cgtgcctgag agcgcagcaa gtttgtacaa aaaagcaggc
      661 tgccaccata acttcgtata ggatacttta tacgaagtta tccggcagaa tggtagctgg
      721 attgtagctg ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac
      781 gaagttatgg cgcggaagtt cctattctct agaaagtata ggaacttccc tcgagtaccg
      841 gtcacgcgta cgcaagcgcc ttagcggccg cttacttgta cagctcgtcc atgccgagag
      901 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
      961 ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
     1021 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
     1081 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
     1141 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
     1201 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
     1261 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
     1321 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
     1381 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
     1441 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
     1501 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
     1561 cggtgaacag ctcctcgccc ttgctcacca tggtggcgga tcctaggtac ctgagctcaa
     1621 cgatagcact agtgaagttc ctattctcta gaaagtatag gaacttcgct agccggataa
     1681 cttcgtataa agtatcctat acgaagttat ttgccttaac ccagaaatta tcactgttat
     1741 tctttagaat ggtgcaaaga ataacttcgt ataatgtatg ctatacgaag ttatacccag
     1801 ctttcttgta caaagtggga attccgataa tcaacctctg gattacaaaa tttgtgaaag
     1861 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     1921 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     1981 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     2041 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     2101 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     2161 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     2221 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     2281 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     2341 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     2401 ttgggccgcc tccccgcatc gggaattcct agagctcgct gatcagcctc gactgtgcct
     2461 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     2521 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     2581 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagag
     2641 aatagcaggc atgctgggga gggccgcagg aacccctagt gatggagttg gccactccct
     2701 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     2761 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     2821 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     2881 gtacgcgccc tgtagcggcg cattaagcgc ggcgggggtg gtggttacgc gcagcgtgac
     2941 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3001 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3061 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     3121 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3181 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
     3241 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
     3301 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     3361 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     3421 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     3481 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     3541 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     3601 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3661 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3721 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3781 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3841 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3901 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3961 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4021 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4081 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4141 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4201 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4261 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4321 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4381 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4441 aagccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4501 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4561 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4621 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4681 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4741 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4801 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4861 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4921 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4981 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5041 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5101 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5161 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5221 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5281 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5341 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5401 catgt
//
