LOCUS       Exported                6714 bp DNA     circular SYN 05-DEC-2018
DEFINITION  .
ACCESSION   VB181205-1162njz
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 8, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=5' ITR
                     /label=nonstandard type:  ITR
                     /note="color: #0375bb; direction: RIGHT"
                     /note="/application_notes=Allows replication of the viral 
                     genomeand its packaging into virus."
                     /note="/description=AAV 5' inverted terminal repeat 
                     (functionalequivalent of wild-type 5' ITR)"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=5f14ec1a645a407dab2725d71bd1c86a"
                     /note="color: #ffc0cb"
     promoter        169..637
                     /label=SYN1
                     /note="color: #a949ca; direction: RIGHT"
                     /note="/application_notes=Tissue specificity: Brain. Cell 
                     typespecificity: Mature neurons."
                     /note="/description=Human synapsin I promoter"
                     /note="/full_name=Human synapsin I promoter"
                     /note="/official_designation=SYN1"
                     /note="/uuid=6f036b2e82244653b8e92867f598e473"
                     /note="color: #1125ff"
     misc_feature    662..667
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="color: #946a00; direction: RIGHT"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
                     /note="color: #ffc0cb"
     misc_feature    complement(668..701)
                     /label=Lox2272
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    752..785
                     /label=loxP
                     /note="color: #214dff"
     misc_feature    792..825
                     /label=FRT
                     /label=FRT(1)
                     /note="color: #00ffff"
     misc_feature    complement(831..1547)
                     /label=EGFP
                     /note="color: #00ff00; direction: LEFT"
     RBS             complement(1551..1616)
                     /label=P2A
                     /note="color: #e6fd18; direction: LEFT"
     CDS             complement(1617..2906)
                     /label=Kir2.1Mut
                     /note="color: #f7cbef; direction: LEFT"
     CDS             complement(2907..2936)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /note="color: #f69b12"
                     /translation="EQKLISEEDL"
     regulatory      complement(2940..2945)
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong 
                     initiationof translation (Kozak, 1987)"
                     /note="/label=vertebrate consensus sequence for strong 
                     initiatio"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    2946..2979
                     /label=FRT
                     /note="color: #00ffff"
     misc_feature    2986..3019
                     /label=lox2272
                     /note="/translation=MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGE
                     GRPYE 
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM 
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG 
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ 
                     YERAEGRHSTGGMDELYK*"
                     /note="color: #214dff"
     misc_feature    complement(3070..3103)
                     /label=loxP
                     /label=loxP(1)
                     /note="/translation=MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGE
                     GRPYE 
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM 
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG 
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ 
                     YERAEGRHSTGGMDELYK*"
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    3134..3731
                     /label=WPRE
                     /label=nonstandard type:  Miscellaneous
                     /note="color: #512bbd; direction: RIGHT"
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis 
                     virusposttranscriptional regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
                     /note="color: #ffc0cb"
     polyA_signal    3762..3969
                     /label=BGH pA
                     /note="color: #d84e4e; direction: RIGHT"
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Bovine growth hormone 
                     polyadenylationsignal"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4a09734ca6c745b3aadc757c1638b651"
                     /note="color: #ffc0cb"
     misc_feature    3977..4117
                     /label=3' ITR
                     /label=nonstandard type:  ITR
                     /note="color: #1f36a9; direction: RIGHT"
                     /note="/application_notes=Allows replication of the viral 
                     genomeand its packaging into virus."
                     /note="/description=AAV 3' inverted terminal repeat"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=5b8101593b634e64aa9eef9434216f71"
                     /note="color: #ffc0cb"
     misc_feature    5034..5894
                     /label=Ampicillin
                     /label=nonstandard type:  ORF
                     /note="color: #db6901; direction: RIGHT"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/description=Ampicillin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=5bcc09480c0e4c07b95ddccd8242d769"
                     /note="color: #ffc0cb"
     rep_origin      6065..6653
                     /label=pUC ori
                     /note="color: #800c0c; direction: RIGHT"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
                     /note="color: #ffc0cb"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttctagacaa ctttgtatag aaaagttgct gcagagggcc
      181 ctgcgtatga gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct
      241 gacgaccgac cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc
      301 cctatcagag agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc
      361 agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac
      421 tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg
      481 tcgcgtccgc gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg
      541 gcacgggcgc gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt
      601 gggcagcgga ggagtcgtgt cgtgcctgag agcgcagcaa gtttgtacaa aaaagcaggc
      661 tgccaccata acttcgtata ggatacttta tacgaagtta tgcagaatgg tagctggatt
      721 gtagctgcta ttagcaatat gaaacctctt aataacttcg tatagcatac attatacgaa
      781 gttatggcgc ggaagttcct attctctaga aagtatagga acttccctta ttacttgtac
      841 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
      901 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
      961 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
     1021 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
     1081 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
     1141 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
     1201 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
     1261 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
     1321 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
     1381 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
     1441 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
     1501 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat aggcccgggg
     1561 ttttcttcaa catctcctgc ttgctttaac agagagaagt tcgtggctcc gcttcctcca
     1621 ctgcctatct ccgattctcg ccttaagggc ctgggctcta gaggtacgct tgcctggttg
     1681 tggagatcta tgccaggagg tgagtctgtg cttgtgctct ctgggactcc gttctcacta
     1741 tcctcctctt cctctttgct tgttagggca acttcatttt catagcaaaa tgaatttgca
     1801 tttgaaagga tgtatttctt ctctgctaag tctctggcac tacaaagggg ggtgttaggt
     1861 acttcataag tcttatggaa tcttgaatag tctactttat agtagtgttt ctcttcaaag
     1921 agcactggct catagcggtg accccagaga atttcattgg ccagatacga actccggcat
     1981 tgagttgtca tggcagtcgc ctccaccatg ccttccagta tgacaacaat ttcaaagtct
     2041 gcattgtcaa tgtcctgctt actcaagtca tataaagggc tgtcttcatc tatttcgtga
     2101 acgatagtga tgggggacac tagaaatatg cggtcaattc cactatcaaa accaacattg
     2161 atgtctatct ggtccaaagg gataaactcc ccttctgaag tgatcctaga tttgagaagc
     2221 tgtgcccgga catgagctcc cacaaggtgg ctctttcgaa ggttacccac tctccacatc
     2281 aagcagagtt tgccatccct catggcaatc acagcattgt gactgaagac aagagtctca
     2341 tttctcttct ttggctttgc catcttcgcc atgactgcac caatgatgaa ggcgtcaatg
     2401 atgcagccta caattgactg gaataccacc atgaagacag caattgggca ttcgtctgtc
     2461 acacacctga aagcagcggc aatggttgtc tgggtctcga tggagaagag gaaggcagcc
     2521 gtgaagctgt taacctccga cacgcatgct ttgctcactt tagaagtatc tagatcccca
     2581 tggagcaggg ctatcaacca aaacacacag ccaaagaaca gccaggagag cacgaaggca
     2641 agacagaaga taaccagcat ccacctccag cggatgtcga cacaggtagt aaagatgtct
     2701 gccaggtacc tctgtccctt ctcacccacg ttgataaact gaacattgca atgaccatct
     2761 ttcttcacaa agcggctcct gcactgttgt cgggtatgga ctttactctt gccattccca
     2821 aagccattgg caactgccat agtggccagc ttcatgccat cttcctccga agagacaatg
     2881 ctgtaccggt tggttctcac actgcccagg tcctcctcgc tgatcagctt ctgctccatg
     2941 gtggcgaagt tcctattctc tagaaagtat aggaacttcg ctagcataac ttcgtataaa
     3001 gtatcctata cgaagttatt tgccttaacc cagaaattat cactgttatt ctttagaatg
     3061 gtgcaaagaa taacttcgta taatgtatgc tatacgaagt tatacccagc tttcttgtac
     3121 aaagtgggaa ttccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     3181 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     3241 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     3301 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     3361 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     3421 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     3481 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     3541 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     3601 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     3661 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     3721 ccccgcatcg ggaattccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     3781 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3841 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     3901 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga atagcaggca
     3961 tgctggggag ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     4021 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     4081 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     4141 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     4201 gtagcggcgc attaagcgcg gcgggggtgg tggttacgcg cagcgtgacc gctacacttg
     4261 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4321 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4381 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     4441 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4501 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
     4561 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4621 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     4681 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4741 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4801 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     4861 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     4921 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4981 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5041 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5101 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5161 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5221 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5281 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5341 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5401 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5461 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5521 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5581 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5641 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5701 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtgga agccgcggta
     5761 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5821 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5881 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5941 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6001 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6061 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6121 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6181 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6241 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6301 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6361 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6421 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6481 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6541 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6601 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6661 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
