LOCUS       Exported                6228 bp DNA     circular SYN 05-DEC-2018
DEFINITION  .
ACCESSION   VB181205-1163mkc
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 8, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=5' ITR
                     /label=nonstandard type:  ITR
                     /note="color: #946a00; direction: RIGHT"
                     /note="/application_notes=Allows replication of the viral 
                     genomeand its packaging into virus."
                     /note="/description=AAV 5' inverted terminal repeat 
                     (functionalequivalent of wild-type 5' ITR)"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=5f14ec1a645a407dab2725d71bd1c86a"
                     /note="color: #ffc0cb"
     promoter        169..637
                     /label=SYN1
                     /note="color: #512bbd; direction: RIGHT"
                     /note="/application_notes=Tissue specificity: Brain. Cell 
                     typespecificity: Mature neurons."
                     /note="/description=Human synapsin I promoter"
                     /note="/full_name=Human synapsin I promoter"
                     /note="/official_designation=SYN1"
                     /note="/uuid=6f036b2e82244653b8e92867f598e473"
                     /note="color: #0a00ff"
     misc_feature    662..667
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="color: #d84e4e; direction: RIGHT"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
                     /note="color: #ffc0cb"
     misc_feature    complement(668..701)
                     /label=Lox2272
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    752..785
                     /label=loxP
                     /note="color: #214dff"
     misc_feature    792..825
                     /label=FRT
                     /label=FRT(1)
                     /note="color: #00ffff"
     misc_feature    complement(831..1547)
                     /label=EGFP
                     /note="color: #00ff00; direction: LEFT"
     RBS             complement(1551..1616)
                     /label=P2A
                     /note="color: #e1fd26; direction: LEFT"
     CDS             complement(1617..2453)
                     /label=NachBac
                     /note="color: #f5000a; direction: LEFT"
     regulatory      complement(2454..2459)
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong 
                     initiationof translation (Kozak, 1987)"
                     /note="/label=vertebrate consensus sequence for strong 
                     initiatio"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    2460..2493
                     /label=FRT
                     /note="color: #00ffff"
     misc_feature    2500..2533
                     /label=lox2272
                     /note="/translation=MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGE
                     GRPYE 
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM 
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG 
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ 
                     YERAEGRHSTGGMDELYK*"
                     /note="color: #214dff"
     misc_feature    complement(2584..2617)
                     /label=loxP
                     /label=loxP(1)
                     /note="/translation=MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGE
                     GRPYE 
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM 
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG 
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ 
                     YERAEGRHSTGGMDELYK*"
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    2648..3245
                     /label=WPRE
                     /label=nonstandard type:  Miscellaneous
                     /note="color: #1f36a9; direction: RIGHT"
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis 
                     virusposttranscriptional regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
                     /note="color: #ffc0cb"
     polyA_signal    3276..3483
                     /label=BGH pA
                     /note="color: #db6901; direction: RIGHT"
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Bovine growth hormone 
                     polyadenylationsignal"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4a09734ca6c745b3aadc757c1638b651"
                     /note="color: #ffc0cb"
     misc_feature    3491..3631
                     /label=3' ITR
                     /label=nonstandard type:  ITR
                     /note="color: #800c0c; direction: RIGHT"
                     /note="/application_notes=Allows replication of the viral 
                     genomeand its packaging into virus."
                     /note="/description=AAV 3' inverted terminal repeat"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=5b8101593b634e64aa9eef9434216f71"
                     /note="color: #ffc0cb"
     misc_feature    4548..5408
                     /label=Ampicillin
                     /label=nonstandard type:  ORF
                     /note="color: #0c5d90; direction: RIGHT"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/description=Ampicillin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=5bcc09480c0e4c07b95ddccd8242d769"
                     /note="color: #ffc0cb"
     rep_origin      5579..6167
                     /label=pUC ori
                     /note="color: #f58600; direction: RIGHT"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
                     /note="color: #ffc0cb"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttctagacaa ctttgtatag aaaagttgct gcagagggcc
      181 ctgcgtatga gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct
      241 gacgaccgac cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc
      301 cctatcagag agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc
      361 agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac
      421 tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg
      481 tcgcgtccgc gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg
      541 gcacgggcgc gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt
      601 gggcagcgga ggagtcgtgt cgtgcctgag agcgcagcaa gtttgtacaa aaaagcaggc
      661 tgccaccata acttcgtata ggatacttta tacgaagtta tgcagaatgg tagctggatt
      721 gtagctgcta ttagcaatat gaaacctctt aataacttcg tatagcatac attatacgaa
      781 gttatggcgc ggaagttcct attctctaga aagtatagga acttccctta ttacttgtac
      841 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
      901 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
      961 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
     1021 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
     1081 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
     1141 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
     1201 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
     1261 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
     1321 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
     1381 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
     1441 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
     1501 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat aggcccgggg
     1561 ttttcttcaa catctcctgc ttgctttaac agagagaagt tcgtggctcc gcttcctcca
     1621 ctgccgatat ccttaagttg tttgagcaag ctcttcagtt cggctacatc ctttcgcaat
     1681 gctgaaatct cctgtttaag tccgtcggcc tccccatctt cctcattgtc cgtaagctcc
     1741 gccttttcca cgttgttcac aatgactccg atgaaaagat tgaaaatgat aaaagtacca
     1801 atcaggacaa acgagacaaa gtagagccat gaccagggca cctctgcaaa aatgggccgc
     1861 attacaccgc tggcccagct ctccaaggtc accacttgga acagcgtcag caacgaaagc
     1921 tggaggttcc caaagtattc aggcgatacg tgctggaaga gcattgtccc gatcactgca
     1981 aagatataga agaagatgga catcaagatc aagatattgc ccaacgccgg aatcgtcatc
     2041 accagcgcat ctacgagtct gcgcagggag gggacgacgg agatcgccct gagtacccgc
     2101 agcactctaa ggattcgcaa tactgtcacg aactgggctc ccgcgaagat atgcccagcc
     2161 gcgacaatca gaaaatcaaa ccaattccac gagctgcgga agaaggccga ctttgggttc
     2221 gacgccaaaa acctcatcgc gatctcgatg gtgaagatcc aaagcaggac gaggtcaatc
     2281 cgataaaaga gccacttgtg gtccgcgtag atcctcggat atgtttcgat tcccacgatc
     2341 agtgcattaa agaggatcag ggcaatgacg gtgaaagtaa acgctctatg gttgacaatt
     2401 ttctgcatct ttgacgtgaa tgagttctgt ttctggcgtg cttccatttt catggtggcg
     2461 aagttcctat tctctagaaa gtataggaac ttcgctagca taacttcgta taaagtatcc
     2521 tatacgaagt tatttgcctt aacccagaaa ttatcactgt tattctttag aatggtgcaa
     2581 agaataactt cgtataatgt atgctatacg aagttatacc cagctttctt gtacaaagtg
     2641 ggaattccga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2701 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2761 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2821 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2881 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2941 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     3001 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     3061 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     3121 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3181 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3241 atcgggaatt cctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     3301 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     3361 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     3421 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gagaatagca ggcatgctgg
     3481 ggagggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     3541 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     3601 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     3661 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     3721 gcgcattaag cgcggcgggg gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3781 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3841 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3901 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     3961 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4021 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
     4081 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     4141 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     4201 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4261 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4321 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     4381 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4441 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4501 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4561 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4621 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4681 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4741 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4801 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4861 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4921 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4981 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5041 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5101 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5161 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5221 ctggctggtt tattgctgat aaatctggag ccggtgagcg tggaagccgc ggtatcattg
     5281 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5341 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5401 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5461 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5521 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5581 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5641 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5701 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5761 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5821 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5881 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5941 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6001 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6061 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6121 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6181 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
