LOCUS       Exported                9788 bp ds-DNA     circular SYN 31-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9788)
  AUTHORS   Kaelin Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 31, 2021 from SnapGene 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9788
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    555..576
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        591..621
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    629..645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     653..669
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        690..708
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        736..962
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             963..1143
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1190..1315
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1808..2041
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2226..2270
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2445..2945
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             2957..3352
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3412..3529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3646..3949
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3950..4153
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    4176..4200
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4984..5008)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5010..5051
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5099..5685
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             5686..6405
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    6428..7016
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6899..6910)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7088..7321
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7399..7520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7560..7695
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7716..7734)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7744..7760)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7902..8357
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8383..8487
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8488..9348
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
       61 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      301 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      361 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
      421 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
      481 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
      541 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      601 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      661 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
      721 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
      781 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
      841 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
      901 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
      961 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     1021 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     1081 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     1141 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     1201 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     1261 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     1321 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     1381 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     1441 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     1501 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     1561 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     1621 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     1681 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     1741 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     1801 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     1861 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     1921 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     1981 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     2041 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     2101 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     2161 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     2221 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     2281 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     2341 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     2401 caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc cttttccaag
     2461 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
     2521 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
     2581 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
     2641 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
     2701 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
     2761 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
     2821 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
     2881 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
     2941 cccagggggt accaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     3001 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     3061 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     3121 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     3181 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     3241 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     3301 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gcctgcagct
     3361 gcagtagtaa gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     3421 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     3481 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     3541 cagggacagc agagatccac tttggcgccg gctcgagggg gcccggggtt attaatagta
     3601 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     3661 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     3721 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     3781 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     3841 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     3901 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     3961 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     4021 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     4081 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     4141 tataagcaga gctctctggc taactgtcgg gatcaacaag tttgtacaaa aaagcaggct
     4201 ccatggccac cccgcccaag agaagctgcc catctttctc agccagctct gaggggaccc
     4261 gcatcaagaa aatctccatc gaagggaaca tcgctgcagg gaagtcaaca tttgtgaata
     4321 tccttaaaca attgtgtgaa gattgggaag tggttcctga acctgttgcc agatggtgca
     4381 atgttcaaag tactcaagat gaatttgagg aacttacaat ggagcagaaa aatggtggga
     4441 atgttcttca gatgatgtat gagaaacctg aacgatggtc ttttaccttc caaacctacg
     4501 cctgtctcag tatgataaga gctcagcttg cctctctgaa tggcaagctc aaagatgcag
     4561 agaaacctgt attatttttt gaacgatctg tgtatagtgc gaggtatatt tttgcatcta
     4621 atttgtatga atctgaatgc atgaatgaga cagagtggac aatttatcaa gactggcatg
     4681 actggatgaa taaccaattt ggccaaagcc ttgaattgga tggaatcatt tatcttcaag
     4741 ccactccaga gacatgctta catagaatat atttacgggg aagaaatgaa gagcaaggca
     4801 ttcctcttga atatttagag aagcttcatt ataaacatga aagctggctc ctgcatagga
     4861 cactgaaaac caacttcgat tatcttcaag aggtgcctat cttaacactg gatgttaatg
     4921 aagactttaa agacaaatat gaaagtctgg ttgaaaaggt caaagagttt ttgagtactt
     4981 tgaacccagc tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
     5041 tcgattctac gtagtaatga gctagccgct acgcgctacg taaattccgc cccccccccc
     5101 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     5161 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     5221 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     5281 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     5341 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     5401 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     5461 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     5521 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     5581 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     5641 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc
     5701 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     5761 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     5821 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     5881 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     5941 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     6001 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     6061 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     6121 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     6181 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     6241 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     6301 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     6361 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaaccgg tggcgcgtta
     6421 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6481 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6541 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6601 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6661 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6721 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6781 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     6841 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6901 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6961 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     7021 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7081 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     7141 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7201 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7261 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7321 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7381 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7441 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7501 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     7561 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7621 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7681 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     7741 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     7801 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     7861 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     7921 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     7981 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     8041 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     8101 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     8161 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     8221 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     8281 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     8341 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8401 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8461 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8521 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8581 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8641 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8701 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8761 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8821 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8881 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8941 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     9001 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     9061 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     9121 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     9181 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     9241 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9301 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9361 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9421 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9481 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9541 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9601 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9661 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9721 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9781 cttaccgg
//
