LOCUS       Exported               10473 bp ds-DNA     circular SYN 31-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Kaelin Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 31, 2021 from SnapGene 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10473
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    555..576
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        591..621
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    629..645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     653..669
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        690..708
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        736..962
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             963..1143
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1190..1315
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1808..2041
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2226..2270
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2445..2945
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             2957..3352
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3412..3529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3646..3949
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3950..4153
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    4176..4200
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4984..5008)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5010..5051
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5096..5648
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             5660..7090
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    7113..7701
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7584..7595)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7773..8006
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8084..8205
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8245..8380
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8401..8419)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8429..8445)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      8587..9042
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9068..9172
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9173..10033
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
       61 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      301 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      361 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
      421 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
      481 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
      541 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      601 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      661 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
      721 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
      781 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
      841 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
      901 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
      961 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     1021 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     1081 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     1141 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     1201 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     1261 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     1321 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     1381 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     1441 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     1501 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     1561 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     1621 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     1681 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     1741 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     1801 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     1861 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     1921 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     1981 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     2041 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     2101 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     2161 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     2221 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     2281 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     2341 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     2401 caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc cttttccaag
     2461 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
     2521 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
     2581 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
     2641 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
     2701 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
     2761 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
     2821 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
     2881 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
     2941 cccagggggt accaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     3001 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     3061 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     3121 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     3181 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     3241 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     3301 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gcctgcagct
     3361 gcagtagtaa gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     3421 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     3481 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     3541 cagggacagc agagatccac tttggcgccg gctcgagggg gcccggggtt attaatagta
     3601 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     3661 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     3721 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     3781 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     3841 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     3901 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     3961 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     4021 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     4081 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     4141 tataagcaga gctctctggc taactgtcgg gatcaacaag tttgtacaaa aaagcaggct
     4201 ccatggccac cccgcccaag agaagctgcc catctttctc agccagctct gaggggaccc
     4261 gcatcaagaa aatctccatc gaagggaaca tcgctgcagg gaagtcaaca tttgtgaata
     4321 tccttaaaca attgtgtgaa gattgggaag tggttcctga acctgttgcc agatggtgca
     4381 atgttcaaag tactcaagat gaatttgagg aacttacaat ggagcagaaa aatggtggga
     4441 atgttcttca gatgatgtat gagaaacctg aacgatggtc ttttaccttc caaacctacg
     4501 cctgtctcag tatgataaga gctcagcttg cctctctgaa tggcaagctc aaagatgcag
     4561 agaaacctgt attatttttt gaacgatctg tgtatagtgc gaggtatatt tttgcatcta
     4621 atttgtatga atctgaatgc atgaatgaga cagagtggac aatttatcaa gactggcatg
     4681 actggatgaa taaccaattt ggccaaagcc ttgaattgga tggaatcatt tatcttcaag
     4741 ccactccaga gacatgctta catagaatat atttacgggg aagaaatgaa gagcaaggca
     4801 ttcctcttga atatttagag aagcttcatt ataaacatga aagctggctc ctgcatagga
     4861 cactgaaaac caacttcgat tatcttcaag aggtgcctat cttaacactg gatgttaatg
     4921 aagactttaa agacaaatat gaaagtctgg ttgaaaaggt caaagagttt ttgagtactt
     4981 tgaacccagc tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
     5041 tcgattctac gtagtaatga gctagccgct acgtaaattc cgcccccccc ccctaacgtt
     5101 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     5161 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     5221 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     5281 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     5341 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     5401 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     5461 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     5521 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     5581 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     5641 tgataatatg gccacaacca tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg
     5701 cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg
     5761 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
     5821 cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt
     5881 gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg
     5941 ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct
     6001 gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg
     6061 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg
     6121 cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta
     6181 cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta
     6241 ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     6301 gtacgagcgc tccgagggcc gccaccacct gttcctgggg catggcaccg gcagcaccgg
     6361 cagcggcagc tccggcaccg cctcctccga ggacaacaac atggccgtca tcaaagagtt
     6421 catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg
     6481 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     6541 cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc
     6601 gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt
     6661 caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc
     6721 ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc
     6781 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
     6841 cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg
     6901 ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg
     6961 ctactactac gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt
     7021 ggaacagtac gagcgctccg agggccgcca ccacctgttc ctgtacggca tggacgagct
     7081 gtacaagtaa accggtggcg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     7141 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7201 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7261 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7321 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7381 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7441 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7501 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7561 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7621 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7681 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     7741 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7801 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     7861 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7921 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7981 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     8041 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     8101 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8161 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     8221 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8281 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8341 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     8401 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     8461 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     8521 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     8581 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     8641 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     8701 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8761 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     8821 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     8881 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     8941 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     9001 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     9061 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     9121 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9181 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9241 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     9301 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     9361 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     9421 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9481 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9541 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9601 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9661 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9721 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9781 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9841 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9901 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9961 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    10021 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    10081 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    10141 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10201 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    10261 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    10321 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    10381 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    10441 tgctgccagt ggcgataagt cgtgtcttac cgg
//
