LOCUS       pAB1678_CMV-HIVNES-GS-C 7833 bp ds-DNA     circular     20-JUN-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        232..819
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     PCR_primer      758..776
                     /label="CGCCCCATTGACGCAAATG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    758..776
                     /label="CMV For"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          769..789
                     /label="CMV forward primer"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     PCR_primer      822..846
                     /label="TGGCTAACTAGAGAACCCACTGCTT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        863..879
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          863..882
                     /label="T7 primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    882..911
                     /label="HindIII GH"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    911..916
                     /label="HindIII"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    917..922
                     /label="Kozak"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             923..925
                     /label="Translation 923-925"
     misc_feature    926..955
                     /label="GH"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             926..970
                     /label="Translation 926-970"
     misc_feature    931..964
                     /label="HIV NES"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    966..969
                     /label="R oligo"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    971..3379
                     /label="Cas13b-t1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             971..3379
                     /label="Translation 971-3379"
     misc_feature    1250..1267
                     /label="HEPN1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3272..3289
                     /label="HEPN2"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3380..3382
                     /label="huADAR2"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             3380..3382
                     /label="Translation 3380-3382"
     misc_feature    complement(3383..3390)
                     /label="NotI"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(3389..3418)
                     /label="NotI GH"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3397..3400
                     /label="Golden Gate"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(3427..3444)
                     /label="BGH reverse primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     polyA_site      3433..3657
                     /label="BGH pA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PCR_primer      complement(3469..3487)
                     /label="AAGGAAGGCACGGGGGAGG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      3703..4131
                     /label="f1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        4136..4505
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             4541..5335
                     /label="Neo(R)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     polyA_signal    5509..5639
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(6022..6692)
                     /label="pUC origin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(6837..7697)
                     /label="Amp(R)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(7698..7796)
                     /label="bla promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccATGggatc cCTTCAACTG CCTCCACTTG AAAGACTGAC
      961 ACTGgGATCC GAATTCGAGA ACATCAAGAA AACCAGCAAC AAAGAGGTGT ACAGCATCGA
     1021 GCAGTACGAG GGCGAGAAGA AGTGGTGCTT CGCCATCGTG CTGAACAGAG CCCAGACCAA
     1081 CCTGGAAGAG AACCCCAAGC TGTTCGAGCA GACCCTGACC AGATTTGAGA AGATCATGAA
     1141 GCAGGACTGG TTCAACGAGG AAACGAAGAA GCTGATCTAC GAGAAAGAGG AAGAAAACAA
     1201 AGTCAAAGAG GAAATCCAGA TCGCCGCCAG CGAGCGGCTG AAGAACCTGA GAAACTACTT
     1261 CAGCCACTAC CTGCACGCCC CTGACTGCCT GATCTTCAAC CGGAACGACA CCATCCGGAT
     1321 CATCATGGAA AAGGCCTACG AGAAGTCCCG CTTCGAGGCC AAGAAGAAGC AGCAAGAGGA
     1381 CATCTCCATC GAGTTCCCCG AGCTGTTTGA GGAAGAGGAC AAGATCACCT CTGCCGGCGT
     1441 GGTGTTCTTC GTCAGCTTTT TTATCGAGCG GCGGTTCCTG AACCGGCTGA TGGGCTATGT
     1501 GCAGGGCTTC AGAAAGACAG AGGGCGAGTA CAACATCACC AGACAGGTGT TCAGCAAGTA
     1561 CTGCCTGAAG GACAGCTACA GCGTGCAGGC CCAGGATCAC GATGCCGTGA TGTTCAGAGA
     1621 CATCCTGGGC TACCTGAGCA GAGTGCCCAC CGAGATCTAC CAGCACATCA AGCTGACCCG
     1681 GAAGCGGAGC CAGGATCAGC TGAGCGAGAG AAAGACCGAC AAGTTCATCC TGTTCGCCCT
     1741 GAAGTACCTC GAGGACTACG GACTGAAGGA CCTGGCCGAT TACACCGCCT GCTTTGCCAG
     1801 AAGCAAGATC AAGAGAGAGA ACGAGGACAC CAAAGAGACA GACGGCAACA AGCACAAGTT
     1861 CCACCGCGAG AAGCCCGTGG TGGAAATCCA CTTCGACAAA GAGAAGCAGG ATCAGTTCTA
     1921 CATCAAGCGG AACAACGTCA TCCTGAAGGC CCAGAAGAAA GGCGGCCAGA GCAACGTGTT
     1981 CCGGATGGGA GTGTACGAGC TGAAGTATCT GGTGCTGCTG AGCCTGCTGG GCAAAGCCGA
     2041 AGAGGCCATC CAGAGAATCG ACCGGTACAT CAGCAGCCTG AAGAAACAGC TGCCCTACCT
     2101 GGATAAGATC AGCAACGAAG AGATCCAGAA GTCCATCAAC TTTCTGCCCA GATTCGTGCG
     2161 GAGCCGGCTG GGACTGCTGC AAGTGGATGA TGAGAAGAGA CTGAAAACCC GGCTGGAATA
     2221 CGTGAAGGCC AAGTGGACCG ATAAGAAAGA GGGCAGCAGA AAGCTGGAAC TGCACCGGAA
     2281 GGGCAGAGAT ATCCTGCGGT ATATCAACGA GAGATGCGAC AGACCCCTGA GCCGGAAAGA
     2341 GTATAACAAC ATTCTGAAGT TCATCGTGAA CAAGGACTTC GCCGGCTTCT ACAACGAGCT
     2401 GGAAGAACTG AAGCGGACCA GACGGCTGGA CAAGAACATC ATCCAGAAGC TGAGCGGCCA
     2461 CACCACACTG AACGCTCTGC ACGAGCGAGT GTGTGACCTG GTCCTGCAAG AGCTGGGCTC
     2521 TCTGCAGTCC GAGAACCTGA AAGAGTACAT CGGACTGATC CCCAAAGAAG AGAAAGAAGT
     2581 CACCTTCCGC GAGAAAGTGG ACAGAATCCT GGAACAGCCT GTGGTGTACA AGGGCTTCCT
     2641 GAGATACGAG TTCTTCAAAG AAGATAAGAA ATCCTTCGCC AGGCTGGTGG AAGAGGCTAT
     2701 CAAGACCAAG TGGAGCGACT TCGACATCCC TCTGGGCGAA GAGTACTACA ACATCCCTAG
     2761 CCTGGACAGA TTCGACCGGA CCAACAAGAA GCTGTACGAG ACACTGGCCA TGGACCGGCT
     2821 GTGTCTGATG ATGGCCAGAC AGTACTACCT GCGGCTGAAC GAGAAGCTGG CCGAGAAGGC
     2881 ACAGCACATC TACTGGAAAA AAGAGGACGG CCGGGAAGTC ATCATCTTCA AGTTCCAGAA
     2941 TCCGAAAGAG CAGAAGAAGT CCTTCAGCAT CAGGTTCAGC ATCCTGGACT ACACCAAGAT
     3001 GTACGTGATG GACGACCCCG AGTTCCTGAG CAGGCTGTGG GAGTACTTCA TCCCTAAAGA
     3061 GGCCAAAGAG ATCGACTACC ACAAGCACTA CGCCAGAGCC TTCGACAAGT ACACCAACCT
     3121 GCAGAAAGAA GGCATCGACG CCATCCTGAA ACTGGAAGGC AGAATCATCG AGCGCCGGAA
     3181 GATCAAGCCC GCCAAGAACT ACATCGAGTT TCAAGAGATT ATGAATCGGA GCGGCTACAA
     3241 CAACGACCAG CAGGTTGCCC TGAAGAGAGT GCGGAACGCC CTGCTGCACT ACAACCTGAA
     3301 CTTCGAGAGA GAGCACCTGA AGCGGTTCTA CGGCGTCGTG AAGAGAGAGG GCATCGAGAA
     3361 AAAATGGTCC CTGATCGTGT GAgcggccgc tcgagtctag agggcccgtt taaacccgct
     3421 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     3481 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     3541 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3601 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3661 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     3721 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3781 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3841 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3901 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3961 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4021 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4081 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     4141 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4201 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4261 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4321 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4381 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     4441 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     4501 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     4561 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     4621 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     4681 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     4741 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     4801 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     4861 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     4921 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     4981 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     5041 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     5101 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     5161 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     5221 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     5281 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     5341 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     5401 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     5461 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     5521 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5581 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     5641 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     5701 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5761 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5821 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5881 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5941 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6001 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6061 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6121 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6181 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6241 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6301 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6361 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6421 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6481 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     6541 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6601 accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6661 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6721 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6781 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6841 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6901 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6961 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7021 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7081 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7141 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7201 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7261 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7321 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7381 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7441 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7501 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7561 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7621 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7681 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7741 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7801 cgcacatttc cccgaaaagt gccacctgac gtc
//