LOCUS       PiggyBac-TetO-Ascl1-ne 10167 bp ds-DNA     circular     03-SEP-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     enhancer        351..352
                     /label="SV40\enhancer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_recomb     complement(449..761)
                     /label="PB\5'LTR"
                     /ApEinfo_revcolor=#8000ff
                     /ApEinfo_fwdcolor=#8000ff
     primer_bind     complement(905..924)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(942..962)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(968..990)
                     /label="LacO"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(1330..2012)
                     /label="ColE1\origin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(2110..2769)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(3101..3556)
                     /label="M13\origin"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      3111..3551
                     /label="F1\ori"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(3561..3629)
                     /label="LacZ\alpha"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     3700..3717
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     3716..3743
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_recomb     complement(3805..4039)
                     /label="PB\3'LTR"
                     /ApEinfo_revcolor=#8000ff
                     /ApEinfo_fwdcolor=#8000ff
     misc_feature    4243..4261
                     /label="tetO2"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    4280..4298
                     /label="tetO2"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    4317..4335
                     /label="tetO2"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    4354..4372
                     /label="tetO2"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    4391..4409
                     /label="tetO2"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    4428..4446
                     /label="tetO2"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    4465..4483
                     /label="tetO2"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    4502..4520
                     /label="tetO2"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    4529..4628
                     /label="mCMV\promoter"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    4529..4634
                     /label="mCMV\promoter"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    4647..4667
                     /label="attB1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4681..4693)
                     /label="attB2"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4683..4688
                     /label="XmaI/AgeI\fused\site"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4695..5390
                     /label="Ascl1\cDNA"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             4695..5390
                     /label="Translation 4695-5390"
     misc_feature    5357..5377
                     /label="taqman F"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    5379..5400
                     /label="taqman probe"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(5412..5866)
                     /label="Rabbit\b-globin\pA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(5453..5472)
                     /label="Taqman R"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    5667..5686
                     /label="genotyping\primer\3F"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(5953..5956)
                     /label="NheI"
                     /ApEinfo_revcolor=#b90746
                     /ApEinfo_fwdcolor=#b90746
     misc_feature    5968..6325
                     /label="pCAG\promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(6137..6144)
                     /label="attB2"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6326..6337
                     /label="CAG_enhancer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6365..6384
                     /label="genotyping\primer\3R"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        6450..7769
                     /label="rEF1a\promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    6535..6554
                     /label="genotyping\primer\1F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    6902..6921
                     /label="genotyping\1R"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             7787..8533
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7816..7835
                     /label="genotyping\primer\2F"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    8126..8145
                     /label="genotyping\primer\2R"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     RBS             8560..9027
                     /label="FMDV\IRES"
                     /ApEinfo_revcolor=#9c663e
                     /ApEinfo_fwdcolor=#9c663e
     promoter        9028..9101
                     /label="EM7\promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             9103..9897
                     /label="neoR\(invivogen)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     polyA_site      join(9943..10167,1..350)
                     /label="EF1\pA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 taatggaaac aacttgacca aaaatttgtc acagaatttt gagacccatt aaaaaagtta
       61 aatgagaaac ctgtgtgttc ctttggtcaa caccgagaca tttaggtgaa agacatctaa
      121 ttctggtttt acgaatctgg aaacttcttg aaaatgtaat tcttgagtta acacttctgg
      181 gtggagaata gggttgtttt ccccccacat aattggaagg ggaaggaata tcatttaaag
      241 ctatgggagg gttgctttga ttacaacact ggagagaaat gcagcatgtt gctgattgcc
      301 tgtcactaaa acaggccaaa aactgagtcc ttgggttgca tagaaagctg ccaaacgcta
      361 gccaacaagc tcgtcatcgc tttgcagaag agcagagagg atatgctcat cgtctaaaga
      421 actacccatt ttattatata ttagtcacga tatctataac aagaaaatat atatataata
      481 agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa
      541 aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat
      601 cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat
      661 gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa
      721 tgcatgcgtc aattttacgc agactatctt tctagggtta aaaaagattt gcgctttact
      781 cgacctaaac tttaaacacg tcatagaatc ttcgtttgac aaaaaccaca ttgtggccaa
      841 gctgtgtgac gcgacgcgcg ctaaagaatg gcaaaccaag tcgcgcgagg tacccagctt
      901 ttgttccctt tagtgagggt taattccgag cttggcgtaa tcatggtcat agctgtttcc
      961 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     1021 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     1081 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1141 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1201 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1261 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1321 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1381 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1441 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1501 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1561 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1621 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1681 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1741 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     1801 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1861 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1921 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1981 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2041 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2101 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2161 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2221 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2281 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2341 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2401 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2461 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2521 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2581 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2641 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2701 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2761 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2821 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2881 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2941 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3001 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3061 aggggttccg cgcacatttc cccgaaaagt gccacctggg aaattgtaaa cgttaatatt
     3121 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     3181 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     3241 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     3301 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     3361 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3421 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     3481 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     3541 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     3601 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     3661 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     3721 ttgtaatacg actcactata gggcgaattg gagctcggta ttcacgacag caggctgaat
     3781 aataaaaaaa ttagaaacta ttatttaacc ctagaaagat aatcatattg tgacgtacgt
     3841 taaagataat catgcgtaaa attgacgcat gtgttttatc ggtctgtata tcgaggttta
     3901 tttattaatt tgaatagata ttaagtttta ttatatttac acttacatac taataataaa
     3961 ttcaacaaac aatttattta tgtttattta tttattaaaa aaaaacaaaa actcaaaatt
     4021 tcttctataa agtaacaaaa cttttaaaca ttctctcttt tacaaaaata aacttatttt
     4081 gtactttaaa aacagtcatg ttgtattata aaataagtaa ttagcttaac ctatacataa
     4141 tagaaacaaa ttatacttat tagtcagtca gaaacaactt tggcacatat caatattatg
     4201 ctctcgttaa ttaactcgag ggatccaagc tggccgcacg tctccctatc agtgatagag
     4261 aagtcgacac gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc
     4321 tatcagtgat agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtacgt
     4381 ctagaacgtc tccctatcag tgatagagaa gtcgacacgt ctcgagctcc ctatcagtga
     4441 tagagaaggt acgtctagaa cgtctcccta tcagtgatag agaagtcgac acgtctcgag
     4501 ctccctatca gtgatagaga aggtaccccc tatataagca gagctcgttt agtgaaccgt
     4561 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
     4621 tccagcctcc gcggtggcgg ccatcacaag tttgtacaaa aaagcaggct ccgcggccgc
     4681 cgaccggggc caccatggag agctctggca agatggagag tggagccggc cagcagccgc
     4741 agcccccgca gcccttcctg cctcccgcag cctgcttctt tgcgaccgcg gcggcggcgg
     4801 cagcggcggc ggccgcggca gctcagagcg cgcagcagca acagccgcag gcgccgccgc
     4861 agcaggcgcc gcagctgagc ccggtggccg acagccagcc ctcagggggc ggtcacaagt
     4921 cagcggccaa gcaggtcaag cgccagcgct cgtcctctcc ggaactgatg cgctgcaaac
     4981 gccggctcaa cttcagcggc ttcggctaca gcctgccaca gcagcagccg gccgccgtgg
     5041 cgcgccgcaa cgagcgcgag cgcaaccggg tcaagttggt caacctgggt tttgccaccc
     5101 tccgggagca tgtccccaac ggcgcggcca acaagaagat gagcaaggtg gagacgctgc
     5161 gctcggcggt cgagtacatc cgcgcgctgc agcagctgct ggacgagcac gacgcggtga
     5221 gcgctgcctt tcaggcgggc gtcctgtcgc ccaccatctc ccccaactac tccaacgact
     5281 tgaactctat ggcgggttct ccggtctcgt cctactcctc cgacgaggga tcctacgacc
     5341 ctcttagccc agaggaacaa gagctgctgg actttaccaa ctggttctga accggtcggg
     5401 cgcgccgatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     5461 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     5521 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     5581 ttggtttaga gtttggcaac atatgccata tgctggctgc catgaacaaa ggtggctata
     5641 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     5701 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     5761 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     5821 agtcatagct gtccctcttc tcttatgaag atccctcgac ctgcaggaat tcgattactt
     5881 aagcctagga tccagatctg tacattcgaa tcgatgaatt cgttaaccat ggaagcttct
     5941 gcagcggccg cactagcggt ggcggcctcg acattgatta ttgactagtt attaatagta
     6001 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     6061 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     6121 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     6181 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     6241 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     6301 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatga tgatgcggtt
     6361 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     6421 ccccattgac gtcaatggga gtttgttttg actagtggag ccgagagtaa ttcatacaaa
     6481 aggagggatc gccttcgcaa ggggagagcc cagggaccgt ccctaaattc tcacagaccc
     6541 aaatccctgt agccgcccca cgacagcgcg aggagcatgc gcccagggct gagcgcgggt
     6601 agatcagagc acacaagctc acagtccccg gcggtggggg gaggggcgcg ctgagcgggg
     6661 gccagggagc tggcgcgggg caaactggga aagtggtgtc gtgtgctggc tccgccctct
     6721 tcccgagggt gggggagaac ggtatataag tgcggtagtc gccttggacg ttctttttcg
     6781 caacgggttt gccgtcagaa cgcaggtgag tggcgggtgt ggcttccgcg ggccccggag
     6841 ctggagccct gctctgagcg ggccgggctg atatgcgagt gtcgtccgca gggtttagct
     6901 gtgagcattc ccacttcgag tggcgggcgg tgcgggggtg agagtgcgag gcctagcggc
     6961 aaccccgtag cctcgcctcg tgtccggctt gaggcctagc gtggtgtccg ccgccgcgtg
     7021 ccactccggc cgcactatgc gttttttgtc cttgctgccc tcgattgcct tccagcagca
     7081 tgggctaaca aagggagggt gtggggctca ctcttaagga gcccatgaag cttacgttgg
     7141 ataggaatgg aagggcagga ggggcgactg gggcccgccc gccttcggag cacatgtccg
     7201 acgccacctg gatggggcga ggcctgtggc tttccgaagc aatcgggcgt gagtttagcc
     7261 tacctgggcc atgtggccct agcactgggc acggtctggc ctggcggtgc cgcgttccct
     7321 tgcctcccaa caagggtgag gccgtcccgc ccggcaccag ttgcttgcgc ggaaagatgg
     7381 ccgctcccgg ggccctgttg caaggagctc aaaatggagg acgcggcagc ccggtggagc
     7441 gggcgggtga gtcacccaca caaaggaaga gggccttgcc cctcgccggc cgctgcttcc
     7501 tgtgaccccg tggtctatcg gccgcatagt cacctcgggc ttctcttgag caccgctcgt
     7561 cgcggcgggg ggaggggatc taatggcgtt ggagtttgtt cacatttggt gggtggagac
     7621 tagtcaggcc agcctggcgc tggaagtcat tcttggaatt tgcccctttg agtttggagc
     7681 gaggctaatt ctcaagcctc ttagcggttc aaaggtattt tctaaacccg tttccaggtg
     7741 ttgtgaaagc caccgctaat tcaaagcaat ccggagtaaa ttcaccatgt ctagactgga
     7801 caagagcaaa gtcataaacg gcgctctgga attactcaat ggagtcggta tcgaaggcct
     7861 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt
     7921 gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac
     7981 ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc
     8041 attccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc
     8101 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt
     8161 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt
     8221 attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc
     8281 tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc
     8341 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag
     8401 cggcgggccg gccgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct
     8461 tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat
     8521 gctccccggg taactaagta aggatcccgt acgcctagga gcaggtttcc ccaatgacac
     8581 aaaacgtgca acttgaaact ccgcctggtc tttccaggtc tagaggggta acactttgta
     8641 ctgcgtttgg ctccacgctc gatccactgg cgagtgttag taacagcact gttgcttcgt
     8701 agcggagcat gacggccgtg ggaactcctc cttggtaaca aggacccacg gggccaaaag
     8761 ccacgcccac acgggcccgt catgtgtgca accccagcac ggcgacttta ctgcgaaacc
     8821 cactttaaag tgacattgaa actggtaccc acacactggt gacaggctaa ggatgccctt
     8881 caggtacccc gaggtaacac gcgacactcg ggatctgaga aggggactgg ggcttctata
     8941 aaagcgctcg gtttaaaaag cttctatgcc tgaataggtg accggaggtc ggcacctttc
     9001 ctttgcaatt actgacccta tgaatacact gactgtttga caattaatca tcggcatagt
     9061 atatcggcat agtataatac gactcactat aggagggcca ccatgattga acaagatgga
     9121 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     9181 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     9241 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     9301 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     9361 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     9421 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     9481 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     9541 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     9601 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     9661 acacatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     9721 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     9781 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     9841 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     9901 ggactctggg gttcgaaatg accgaccaag cgaattcgct aggattatcc ctaatacctg
     9961 ccaccccact cttaatcagt ggtggaagaa cggtctcaga actgtttgtt tcaattggcc
    10021 atttaagttt agtagtaaaa gactggttaa tgataacaat gcatcgtaaa accttcagaa
    10081 ggaaaggaga atgttttgtg gaccactttg gttttctttt ttgcgtgtgg cagttttaag
    10141 ttattagttt ttaaaatcag tactttt
//