LOCUS       PiggyBac-TRE-En1-t2a-F 11294 bp ds-DNA     circular     03-SEP-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        59..1247
                     /label="EF1a\promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1248..1250
                     /label="short\kozak"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1251..1997
                     /label="rtTA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          1993..2082
                     /label="SM_t2a_Hyg_FW"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2001..2054
                     /label="t2a"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             2061..3086
                     /label="Hygromycin"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     polyA_signal    3096..3226
                     /label="SV40\PolyA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(3474..3704)
                     /label="Insulator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     LTR             3979..4013
                     /label="3'TR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(7337..7356)
                     /label="BII(R)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     LTR             7399..7461
                     /label="5'TR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    7638..7869
                     /label="Insulator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    7895..8336
                     /label="TRE"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    8349..9551
                     /label="mEn1\(no\stop)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    9424..9443
                     /label="genotyping\primer\2F"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    9521..9540
                     /label="genotyping\primer\1F"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    9558..9635
                     /label="t2a"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    9648..11021
                     /label="hFoxA2"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    9695..9714
                     /label="genotyping\primer\2R"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    9828..9847
                     /label="genotyping\primer\1R"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    join(11042..11294,1..50)
                     /label="bGH\polyA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 tgaggcggaa agaaccagct ggggctcgac tagagcttgc ggaacccttc gcggccgccg
       61 tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
      121 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
      181 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
      241 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
      301 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
      361 attacttcca cgcccctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
      421 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
      481 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
      541 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
      601 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
      661 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
      721 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
      781 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
      841 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
      901 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
      961 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     1021 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     1081 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     1141 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     1201 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgagacc atgtctagac
     1261 tggacaagag caaagtcata aacggcgctc tggaattact caatggagtc ggtatcgaag
     1321 gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc ctgtactggc
     1381 acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg gacaggcatc
     1441 atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg aacaacgcca
     1501 agtcattccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat ctcggcaccc
     1561 gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag
     1621 gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt acactgggct
     1681 gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca cctaccaccg
     1741 attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag ggagccgaac
     1801 ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag ctaaagtgcg
     1861 aaagcggcgg gccggccgac gcccttgacg attttgactt agacatgctc ccagccgatg
     1921 cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat tttgaccttg
     1981 acatgctccc cgggggatcc gagggcaggg gaagtcttct aacatgcggg gacgtggagg
     2041 aaaatcccgg cccagctagc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     2101 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     2161 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     2221 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     2281 ttccggaagt gcttgacatt ggggaattta gcgagagcct gacctattgc atctcccgcc
     2341 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     2401 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     2461 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     2521 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     2581 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     2641 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     2701 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     2761 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     2821 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     2881 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     2941 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     3001 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     3061 gcactcgtcc gagggcaaag gaataggtcg acgttaactt gtttattgca gcttataatg
     3121 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3181 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggaat tgactcaaat
     3241 gatgtcaatt agtctatcag aagctatctg gtctcccttc cgggggacaa gacatccctg
     3301 tttaatattt aaacagcagt gttcccaaac tgggttctta tatcccttgc tctggtcaac
     3361 caggttgcag ggtttcctgt cctcacagga acgaagtccc taaagaaaca gtggcagcca
     3421 ggtttagccc cggaattgac tggattcctt ttttagggcc cattggtatg gctttttccc
     3481 cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga
     3541 tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg
     3601 ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag
     3661 ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctgatatc tataacaaga
     3721 aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt
     3781 atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt
     3841 cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc
     3901 aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag
     3961 agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaatct
     4021 agctgcatca ggatcatatc gtcgggtctt ttttccggct cagtcatcgc ccaagctggc
     4081 gctatctggg catcggggag gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat
     4141 ggaaagagtt tgccgaggat gactgctgct gcattgacgt tgagcgaaaa cgcacgttta
     4201 ccatgatgat tcgggaaggt gtggccatgc acgcctttaa cggtgaactg ttcgttcagg
     4261 ccacctggga taccagttcg tcgcggcttt tccggacaca gttccggatg gtcagcccga
     4321 agcgcatcag caacccgaac aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga
     4381 ttaatgacag cggtgcggcg ctgggatatt acgtcagcga ggacgggtat cctggctgga
     4441 tgccgcagaa atggacatgg ataccccgtg agttacccgg cgggcgcgct tggcgtaatc
     4501 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     4561 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     4621 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     4681 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     4741 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4801 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4861 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4921 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4981 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5041 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5101 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5161 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5221 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5281 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5341 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5401 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5461 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5521 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5581 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5641 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5701 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5761 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5821 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5881 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5941 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     6001 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6061 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6121 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     6181 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     6241 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     6301 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     6361 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6421 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6481 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6541 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6601 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     6661 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     6721 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     6781 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     6841 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     6901 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     6961 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     7021 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     7081 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
     7141 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     7201 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     7261 acggccagtg agcgcgcctc gttcattcac gtttttgaac ccgtggagga cgggcagact
     7321 cgcggtgcaa atgtgtttta cagcgtgatg gagcagatga agatgctcga cacgctgcag
     7381 aacacgcagc tagattaacc ctagaaagat aatcatattg tgacgtacgt taaagataat
     7441 catgcgtaaa attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt
     7501 tgaatagata ttaagtttta ttatatttac acttacatac taataataaa ttcaacaaac
     7561 aatttattta tgtttattta tttattaaaa aaaaacaaaa actcaaaatt tcttctataa
     7621 agtaacaaaa cttttatgag ggacagcccc cccccaaagc ccccagggat gtaattacgt
     7681 ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt ccggcgctcc
     7741 ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg
     7801 gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tggggggata
     7861 cggggaaaag gcctccaagg ccactagtct cgagtttacc actccctatc agtgatagag
     7921 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     7981 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     8041 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     8101 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     8161 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgagtagg cgtgtacggt
     8221 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     8281 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat
     8341 tcgccaccat ggaagaacag cagccggagc ctaaaagtca gcgcgactcg ggcctcggcg
     8401 cggtggcagc ggcggccccg agcggcctca gtctgagtct gagcccagga gccagcggca
     8461 gcagcggcag cgatggagac agcgtgccgg tgtccccgca gccagcgccc ccgtcgcctc
     8521 ctgcggcacc ctgtctgccg cccctggccc atcacccgca cctccccccg catcccccgc
     8581 ccccgccgcc gccgccgccg ccgccaccgc agcatctcgc ggcgcctgct caccagccgc
     8641 agcccgcggc ccagctgcac cgcaccacca actttttcat cgataacatc ctaaggcccg
     8701 atttcggttg caaaaaggaa cagcccctgc ctcagctcct ggtggcttcg gctgcagccg
     8761 gaggaggcgc agcagcagga ggaggaagcc gcgtggagcg tgaccgaggc cagactggtg
     8821 caggtagaga ccccgttcac tctctgggca cacgagcttc gggggctgcc tcgctcttgt
     8881 gtgctccaga tgcgaactgt ggcccacccg acggctccca gcccgccacc gctgtcggcg
     8941 ccggcgcatc caaagccggg aacccggctg ctgcggcggc cgcggccgca gcagcggctg
     9001 cagcggcagt ggcggcagcg gcggcagcag cctcgaagcc ctcggacagt ggcggtggta
     9061 gtggaggcaa cgcggggagt cccggggcgc agggcgccaa gttcccggaa cacaaccctg
     9121 cgatcctact catgggttcg gctaacggtg ggccggtggt caagactgac tcacagcaac
     9181 ccctagtgtg gcccgcctgg gtctactgca cacgctattc ggaccgtccg tcctctggtc
     9241 cacgcaccag gaagctaaag aagaaaaaga acgagaagga agacaagcgg ccgcggacgg
     9301 cgttcacggc cgagcagctg cagagactca aggcggagtt ccaggcaaac cgctatatca
     9361 cggagcagcg gcgacagacc ctcgcccagg agctcagcct gaatgagtcc cagatcaaga
     9421 tctggttcca aaacaagcgt gccaagatca agaaagccac aggcatcaag aacggcctgg
     9481 cgctgcacct catggcccag ggactgtaca accactctac caccacggtt caggacaaag
     9541 acgagagcga gggatccatg gctagcggct ccggcgaggg caggggaagt cttctaacat
     9601 gcggggacgt ggaggaaaat cccggcccag catgctctag agccaccatg ctgggagcgg
     9661 tgaagatgga agggcacgag ccgtccgact ggagcagcta ctatgcagag cccgagggct
     9721 actcctccgt gagcaacatg aacgccggcc tggggatgaa cggcatgaac acgtacatga
     9781 gcatgtcggc ggccgccatg ggcagcggct cgggcaacat gagcgcgggc tccatgaaca
     9841 tgtcgtcgta cgtgggcgct ggcatgagcc cgtccctggc ggggatgtcc cccggcgcgg
     9901 gcgccatggc gggcatgggc ggctcggccg gggcggctgg cgtggcgggc atggggccgc
     9961 acttgagtcc cagcctgagc ccgctcgggg ggcaggcggc cggggccatg ggcggcctgg
    10021 ccccctacgc caacatgaac tccatgagcc ccatgtacgg gcaggcgggc ctgagccgcg
    10081 cccgcgaccc caagacctac aggcgcagct acacgcacgc aaagccgccc tactcgtaca
    10141 tctcgctcat caccatggcc atccagcaga gccccaacaa gatgctgacg ctgagcgaga
    10201 tctaccagtg gatcatggac ctcttcccct tctaccggca gaaccagcag cgctggcaga
    10261 actccatccg ccactcgctc tccttcaacg actgtttcct gaaggtgccc cgctcgcccg
    10321 acaagcccgg caagggctcc ttctggaccc tgcaccctga ctcgggcaac atgttcgaga
    10381 acggctgcta cctgcgccgc cagaagcgct tcaagtgcga gaagcagctg gcgctgaagg
    10441 aggccgcagg cgccgccggc agcggcaaga aggcggccgc cggggcccag gcctcacagg
    10501 ctcaactcgg ggaggccgcc gggccggcct ccgagactcc ggcgggcacc gagtcgcctc
    10561 actcgagcgc ctccccgtgc caggagcaca agcgaggggg cctgggagag ctgaagggga
    10621 cgccggctgc ggcgctgagc cccccagagc cggcgccctc tcccgggcag cagcagcagg
    10681 ccgcggccca cctgctgggc ccgccccacc acccgggcct gccgcctgag gcccacctga
    10741 agccggaaca ccactacgcc ttcaaccacc cgttctccat caacaacctc atgtcctcgg
    10801 agcagcagca ccaccacagc caccaccacc accagcccca caaaatggac ctcaaggcct
    10861 acgaacaggt gatgcactac cccggctacg gttcccccat gcctggcagc ttggccatgg
    10921 gcccggtcac gaacaaaacg ggcctggacg cctcgcccct ggccgcagat acctcctact
    10981 accagggggt gtactcccgg cccattatga actcctctta gttaattaag ctagccctag
    11041 gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
    11101 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
    11161 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
    11221 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
    11281 ggctctatgg cttc
//