LOCUS       CRY1_gRNA2_backbone     5290 bp ds-DNA     circular     27-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      170..476
                     /label="f1 Origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    995..1852
                     /label="AmpR"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     rep_origin      2003..2670
                     /label="pUC ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2676..2816
                     /label="L-ITR"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    2714..2724
                     /label="sequencing error part"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2826..2830
                     /label="MluI"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     promoter        2831..3079
                     /label="Human U6 Promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2899..3663
                     /label="hU6 sequencing"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3077..3079
                     /label="5'"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    3080..3099
                     /label="CRY1 gRNA2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3100..3102
                     /label="3'"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    3100..3181
                     /label="chimeric RNA backbone 1 (SapI)"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    3182..3187
                     /label="XbaI"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    3182..3663
                     /label="hSyn"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    complement(3250..3269)
                     /label="hsyn_reverse"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3691..4395
                     /label="hSyn-F (2 mis)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     exon            3691..4395
                     /label="mCherry"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             4420..4668
                     /label="KASH domain of Nesprin2"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    4677..4690
                     /label="WPRE"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     polyA_signal    4710..5188
                     /label="hGH polyA"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    join(5228..5290,1..78)
                     /label="R-ITR"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
ORIGIN
        1 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
       61 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
      121 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
      181 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      241 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      301 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
      361 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
      421 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
      481 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
      541 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
      601 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
      661 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
      721 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
      781 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      841 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      901 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      961 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1021 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1081 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1141 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1201 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     1261 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1321 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1381 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1441 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1501 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1561 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1621 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1681 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1741 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1801 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     1861 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1921 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1981 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2041 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2101 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2161 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2221 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2281 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2341 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2401 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2461 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2521 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2581 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2641 gttcctggcc ttttgctggc cttttgctca catgtcctgc aggcagctgc gcgctcgctc
     2701 gctcactgag gccgcccggg caaagcccgg gcgtcgggcg acctttggtc gcccggcctc
     2761 agtgagcgag cgagcgcgca gagagggagt ggccaactcc atcactaggg gttcctgcgg
     2821 ccgcacgcgt gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
     2881 tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
     2941 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
     3001 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
     3061 GTGGAAAGGA CGAAACACCG GTCGAGGATA TAGACGCAGg ttttagagct aGAAAtagca
     3121 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcTTTTT
     3181 Ttctagactg cagagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc
     3241 ggggtggggg tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca
     3301 ttccccaaat tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg
     3361 cgtgcgcact gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc
     3421 gccaccgccg cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc
     3481 cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg
     3541 cgaggcgcga gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag
     3601 cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga
     3661 gaaggtaccg gatcctctag agTCGACatg gtgagcaagg gcgaggagga taacatggcc
     3721 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     3781 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     3841 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     3901 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     3961 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     4021 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     4081 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     4141 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     4201 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     4261 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     4321 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     4381 gacgagctgt acaagGAATT Ctccggactc agatctcgag aggaggagga ggagacagac
     4441 agcaggatgc cccacctcga cagccccggc agctcccagc cgagacgctc cttcctctca
     4501 agggtgatca gggcagcgct accgttgcag ctgcttctgc tgctgctgct gctcctggcc
     4561 tgcctgctac ctgcctctga agatgactac agctgcaccc aggccaacaa ctttgcccga
     4621 tccttctacc ccatgctgcg gtacaccaac gggccacctc ccacctagGA Tatcaagctt
     4681 atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt
     4741 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     4801 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     4861 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     4921 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     4981 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     5041 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     5101 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     5161 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag
     5221 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     5281 actgaggccg
//