LOCUS       Exported               11102 bp DNA     circular SYN 11-JAN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Princeton Network
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11102
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     intron          455..520
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /note="color: #------"
     CDS             650..670
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    1095..1229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(1132..1151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     1186..1205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     LTR             1397..2030
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    2077..2202
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    2699..2932
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    3446..3563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     3789..3813
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
                     /note="color: black; sequence: AGCTCGTTTAGTGAACCGTCAGATC"
     primer_bind     3879..3911
                     /label=dGFP insert.FOR
                     /note="color: black; sequence: 
                     gacattcgttggatcatggtgagcaagggcgag"
     primer_bind     3893..3911
                     /label=d2EGFP insert.FOR
                     /note="color: black; sequence: 
                     TCCCCAAACTTCGACatggtgagcaagggcgag"
     CDS             3894..4739
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 5 segments:
                        1: 3894 .. 3896 / #05fd14
                        2: 3897 .. 3899 / #05fd14 / 1a
                        3: 3900 .. 4610 / #05fd14 / EGFP
                        4: 4611 .. 4616 / #05fd14
                        5: 4617 .. 4739 / #05fd14 / destabilization domain"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     regulatory      3894..3897
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     primer_bind     4611..4631
                     /label=synIEG deg protein replace.FOR
                     /note="color: black; sequence: aagcttagccatggcttcccg"
     primer_bind     complement(4712..4754)
                     /label=dGFP insert.REV
                     /note="color: black; sequence: 
                     cgcaagtcaaggatcctacacattgatcctagcagaagcacag"
     primer_bind     complement(4712..4739)
                     /label=d2 EGFP.REV
                     /note="color: black; sequence: 
                     CGAATTCGCGGCttactacacattgatcctagcagaagcacag"
     promoter        4862..5361
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(4897..4920)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
                     /note="color: black; sequence: CAGCGGGGCTGCTAAAGCGCATGC"
     misc_feature    5263..5282
                     /label=gRNA 1
                     /note="color: #a6acb3"
     primer_bind     5294..5313
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
                     /note="color: black; sequence: CATTCTGCACGCTTCAAAAG"
     primer_bind     complement(5342..5381)
                     /label=gal4 pgk bb (irfp) R
                     /note="color: black; sequence: 
                     ggtaagcttgggctgcaggtcgaaAGGCCCGGAGATGAGG"
     primer_bind     5367..5410
                     /label=irfp ins (gal4 pgk) F
                     /note="color: black; sequence: 
                     cagcccaagcttaccatggctgaaggAtccgtcgcCAGGCAGCC"
     CDS             5382..6329
                     /codon_start=1
                     /gene="irFP"
                     /label=iRFP
                     /note="CDS "
                     /note="color: #ff00ff"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     primer_bind     complement(6290..6344)
                     /label=irfp ins (gal4 pgk) R
                     /note="color: black; sequence: 
                     gtcgcggccgctttactcttccatcacgccgatctgccagGCCAGAACCTGGGCG"
     primer_bind     complement(6302..6337)
                     /label=KTR irFP bb (new h2b) R
                     /note="color: black; sequence: 
                     ggctgaagttagtagctccgcttccctcttccatcacgccgatctgccaggcc"
     primer_bind     6330..6372
                     /label=gal4 pgk bb (irfp) F
                     /note="color: black; sequence: 
                     taaagcggccgcgactctagagtcgACCTGCAGGCATGCAAGC"
     misc_feature    6409..6997
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(6462..6482)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     CDS             complement(6880..6891)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 6879"
                     /translation="IEGR"
     LTR             7060..7293
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        complement(7318..7336)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(7319..7336)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
                     /note="color: black; sequence: ATTTAGGTGACACTATAG"
     primer_bind     complement(7626..7645)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
                     /note="color: black; sequence: GGTGATGTCGGCGATATAGG"
     primer_bind     complement(7859..7878)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /note="color: black; sequence: CGGCATCAGAGCAGATTGTA"
     primer_bind     7978..8000
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
                     /note="color: black; sequence: CCGGGAGCTGCATGTGTCAGAGG"
     primer_bind     complement(8038..8056)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /note="color: black; sequence: AATAGGCGTATCACGAGGC"
     promoter        8124..8228
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             8229..9089
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8229 .. 8297 / #ccffcc / signal sequence
                        2: 8298 .. 9089 / #ccffcc
                     Cleavage site after base 8297"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8447..8466)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     rep_origin      9260..9848
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     9749..9768
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     primer_bind     10002..10019
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     primer_bind     complement(10089..10109)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /note="color: black; sequence: ACCCTAACTGACACACATTCC"
     promoter        10094..10423
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      10274..10409
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     10336..10355
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     primer_bind     complement(10359..10370)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
ORIGIN
        1 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
       61 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
      121 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
      181 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
      241 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
      301 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
      361 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
      421 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
      481 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
      541 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
      601 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
      661 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
      721 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
      781 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
      841 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
      901 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
      961 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     1021 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     1081 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     1201 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     1261 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     1321 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     1381 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     1441 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     1501 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     1561 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     1621 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     1681 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     1741 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     1801 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     1861 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1921 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1981 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     2041 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     2101 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     2161 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     2221 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     2281 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     2341 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     2401 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     2461 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     2521 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     2581 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     2641 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     2701 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     2761 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     2821 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     2881 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     2941 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     3001 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     3061 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     3121 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     3181 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     3241 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     3301 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     3361 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     3421 gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     3481 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     3541 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga
     3601 tcgataagct tgatatcgaa ttcggagcac tgtcctccga acgtcggagc actgtcctcc
     3661 gaacgtcgga gcactgtcct ccgaacgtcg gagcactgtc ctccgaacgg agcatgtcct
     3721 ccgaacgtcg gagcactgtc ctccgaacga ctagttaggc gtgtacggtg ggaggcctat
     3781 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     3841 gacctccata gaagacaccg ggaccgatcc agcctctcga cattcgttgg atcatggtga
     3901 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3961 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4021 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4081 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     4141 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4201 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4261 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4321 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     4381 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4441 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4501 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4561 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag aagcttagcc
     4621 atggcttccc gccggaggtg gaggagcagg atgatggcac gctgcccatg tcttgtgccc
     4681 aggagagcgg gatggaccgt caccctgcag cctgtgcttc tgctaggatc aatgtgtagg
     4741 atccttgact tgcggccgca actcccacct gcaacatgcg tgactgactg aggccgcgac
     4801 tctagagtcg acctgcatct aggcgccgga attagatctc tcgaggttaa cgaattctac
     4861 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     4921 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     4981 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     5041 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     5101 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     5161 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     5221 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     5281 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     5341 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catggctgaa ggatccgtcg
     5401 ccaggcagcc tgacctcttg acctgcgacg atgagccgat ccatatcccc ggtgccatcc
     5461 aaccgcatgg actgctgctc gccctcgccg ccgacatgac gatcgttgcc ggcagcgaca
     5521 accttcccga actcaccgga ctggcgatcg gcgccctgat cggccgctct gcggccgatg
     5581 tcttcgactc ggagacgcac aaccgtctga cgatcgcctt ggccgagccc ggggcggccg
     5641 tcggagcacc gatcactgtc ggcttcacga tgcgaaagga cgcaggcttc atcggctcct
     5701 ggcatcgcca tgatcagctc atcttcctcg agctcgagcc tccccagcgg gacgtcgccg
     5761 agccgcaggc gttcttccgc cgcaccaaca gcgccatccg ccgcctgcag gccgccgaaa
     5821 ccttggaaag cgcctgcgcc gccgcggcgc aagaggtgcg gaagattacc ggcttcgatc
     5881 gggtgatgat ctatcgcttc gcctccgact tcagcggcga agtgatcgca gaggatcggt
     5941 gcgccgaggt cgagtcaaaa ctaggcctgc actatcctgc ctcaaccgtg ccggcgcagg
     6001 cccgtcggct ctataccatc aacccggtac ggatcattcc cgatatcaat tatcggccgg
     6061 tgccggtcac cccagacctc aatccggtca ccgggcggcc gattgatctt agcttcgcca
     6121 tcctgcgcag cgtctcgccc gtccatctgg aattcatgcg caacataggc atgcacggca
     6181 cgatgtcgat ctcgattttg cgcggcgagc gactgtgggg attgatcgtt tgccatcacc
     6241 gaacgccgta ctacgtcgat ctcgatggcc gccaagcctg cgagctagtc gcccaggttc
     6301 tggcctggca gatcggcgtg atggaagagt aaagcggccg cgactctaga gtcgacctgc
     6361 aggcatgcaa gcttgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg
     6421 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6481 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6541 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6601 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6661 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6721 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6781 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     6841 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6901 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6961 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg accaaggcag
     7021 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7081 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7141 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7201 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7261 gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct
     7321 atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg
     7381 ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct
     7441 atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc
     7501 tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag
     7561 ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     7621 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     7681 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     7741 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     7801 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta
     7861 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     7921 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     7981 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     8041 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     8101 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8161 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8221 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8281 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8341 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8401 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8461 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     8521 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     8581 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     8641 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     8701 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     8761 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     8821 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     8881 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     8941 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9001 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9061 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9121 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9181 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9241 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9301 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9361 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     9421 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9481 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     9541 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     9601 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     9661 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     9721 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     9781 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     9841 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     9901 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     9961 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10021 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    10081 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
    10141 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    10201 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    10261 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    10321 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    10381 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
    10441 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
    10501 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
    10561 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
    10621 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
    10681 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
    10741 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
    10801 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
    10861 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
    10921 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
    10981 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
    11041 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
    11101 gc
//
