LOCUS       pHS0806_pET45b(+)_His14 6760 bp ds-DNA     linear       04-JUL-2021
DEFINITION  .
KEYWORDS    "creator:Broad Institute" "marker:AmpR, AmpR"                      
FEATURES             Location/Qualifiers
     promoter        complement(1..14)
                     /label="lacI promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        327..345
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    complement(346..370)
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    383..416
                     /label="NcoI homology"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    386..406
                     /label="RBS"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             complement(401..406)
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             414..416
                     /label="ATG"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             414..2111
                     /label="Translation 414-2111"
     CDS             423..485
                     /label="His14"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             489..779
                     /label="bdSUMO"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             780..791
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             792..2108
                     /label="AwaIscB"
                     /ApEinfo_revcolor=#666699
                     /ApEinfo_fwdcolor=#666699
     CDS             792..2108
                     /label="Translation 792-2108"
     misc_feature    1263..1265
                     /label="RuvCII"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1428..1430
                     /label="HNH"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(2113..2132)
                     /label="PacI homology"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      complement(2145..2192)
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      2229..2684
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(2799..3590)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2940..2942
                     /label="BsaI site removed"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(3591..3659)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      3833..4421
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(4851..5042)
                     /label="rop"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             complement(5614..6696)
                     /label="lacI"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     promoter        complement(6697..6760)
                     /label="lacI promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 CATTCGATGG TGTCCGGGAT CTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA
       61 GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA
      121 GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC
      181 GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC
      241 GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT
      301 CGAGATCGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGGAAT TGTGAGCGGA
      361 TAACAATTCC CCTCTAGAAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACCATGagca
      421 agcatcacca tcattcaggc catcaccata ccggacacca ccatcattca ggcagtcatc
      481 accataccGG ATCTGCTGCG GGTGGCGAAG AAGATAAGAA ACCGGCAGGT GGCGAAGGTG
      541 GCGGTGCCCA TATCAACCTG AAAGTGAAAG GTCAAGACGG CAACGAAGTC TTTTTCCGCA
      601 TCAAACGTTC TACCCAGCTG AAAAAGCTGA TGAACGCATA CTGTGACCGT CAGTCTGTAG
      661 ACATGACCGC AATTGCTTTC CTGTTTGATG GTCGTCGCCT GCGTGCGGAA CAGACCCCGG
      721 ATGAACTGGA GATGGAAGAT GGCGACGAAA TCGACGCAAT GCTGCACCAG ACTGGTGGCT
      781 CAAGTGGATC CATGAGCGTG TTCGTGCTGA ACAAGCAGAA ACGGCCTCTG ATGCCCTGCA
      841 GCGAGAAGAG AGCCCGCCTG CTGCTGGAAA GAGGCAGAGC CGTGGTGGTG CGGCTGGTGC
      901 CATTCACCAT CCGGCTGAAG GATAGAATCG GCGGCGTGCT GCAGCCCCTG AGACTGAAGC
      961 TGGACCCTGG CTCTAAGACA ACCGGCATCG CCCTGGTGCG CGAGGTGGTG CGGCGGGACG
     1021 AGAGCGTGGT TTGGCTGGCT GAACTGACAC ACAGAGGATA TCAGATCAGC GAGGCCCTGA
     1081 GAGCTAGAAG CGCCATGAGA AGAAGAAGAA GGTCCGCCAA CCTGCGGTAC AGAGCCCCTA
     1141 GATTCCTGAA TCGGACCAAG CCTAAGGGCT GGCTGGCCCC TAGCCTGAGA CATAGAGTGG
     1201 AAACCACCAT TAACTGGGTC AAGAGACTGC GGAGACTGGC TCCTATCACG GAAATCACCC
     1261 AGGAGCTGGT CAGGTTCGAC CTGCAAGCCA TGCAGCACCC CGAGATCAGC GGCATTGAGT
     1321 ACCAGCAGGG CGAGCTGGCT GGCTACGAGG TGCGGGAATA CCTGCTGGAA AAGTGGCAGC
     1381 GGACATGCGC CTACTGCGGC GCCCAGCAGG TGCCCCTCCA GATCGAGCAC ATCAGACCTA
     1441 AGTCCGCCGG CGGAAGCAAC AGATTGTCTA ATCTCACCCT GGCTTGCGCC CCTTGCAACC
     1501 ACAAGAAGGG CGCTCAATCT ATCGAGGCCT TCCTGAAGCA CAAACTGGAA CTGCTGAAAC
     1561 AGATCCAGGC CCAGGCACAA GCCCCTCTGA AAGACGCCGC CGCCGTGAAC ACCACCCGGT
     1621 GGGCCCTGTT CAACGCCCTG AAGGCTACAG GCCTGCAGGT GAAAACCGGC AGCGGCGGAC
     1681 AGACCAAATA CAACCGCCAG AGACTGGGCA TCCCAAAGAC CCACGCCCTG GACGCCGCTT
     1741 GTGTGGGCAA GCTGGATGCC CTGCACAACT GGCAGATCCC TACACTGGCC ATCAAGGCGA
     1801 TGGGCAGAGG CAGCTACCAG AGGACCAGAC TGAATAGATT CGGCTTCCCC AGAGGCCACC
     1861 TGATGAGACA CAAGAGAATC CACGGTTTTC AGACCGGCGA CAGAGTGATC GCACACATCC
     1921 CCAGCGGCAA AAAGGCCGGA GTGCACGTGG GAAGAGTGGC CGTCCGAACC AGCGGATCTT
     1981 TTAACATCCA AACAGCCACA GGCGTGATCC AGGGCATCGC CCACCGGCAT TGTAGCGTGC
     2041 TGCAGCGGGC CGACGGCTAC GGCTACAGCT TCAACCTGAC CCAGCCTGAG GAAGCCAGAC
     2101 TGGCTGCCTG ATTAACCTAG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG
     2161 GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATTGG
     2221 CGAATGGGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG
     2281 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT
     2341 TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT
     2401 CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG
     2461 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT
     2521 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT
     2581 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA
     2641 AAAATTTAAC GCGAATTTTA ACAAAATATT AACGTTTACA ATTTCTGGCG GCACGATGGC
     2701 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     2761 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     2821 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     2881 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGg
     2941 GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     3001 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     3061 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     3121 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     3181 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     3241 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     3301 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     3361 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     3421 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     3481 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     3541 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     3601 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     3661 CTCTTCCTTT TTCAATCATG ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     3721 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGTCAT GACCAAAATC CCTTAACGTG
     3781 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC
     3841 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG
     3901 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG
     3961 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT
     4021 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
     4081 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC
     4141 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG
     4201 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG
     4261 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG
     4321 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
     4381 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT
     4441 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC
     4501 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC
     4561 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT
     4621 TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT
     4681 CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC
     4741 ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC
     4801 CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT
     4861 TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA
     4921 AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC
     4981 GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC
     5041 ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA
     5101 GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT
     5161 GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA
     5221 TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG
     5281 ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA
     5341 ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG
     5401 TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC
     5461 CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC TAGTCATGCC CCGCGCCCAC
     5521 CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGAGATCC CGGTGCCTAA
     5581 TGAGTGAGCT AACTTACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC
     5641 CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT
     5701 GGGCGCCAGG GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT TGCCCTTCAC
     5761 CGCCTGGCCC TGAGAGAGTT GCAGCAAGCG GTCCACGCTG GTTTGCCCCA GCAGGCGAAA
     5821 ATCCTGTTTG ATGGTGGTTA ACGGCGGGAT ATAACATGAG CTGTCTTCGG TATCGTCGTA
     5881 TCCCACTACC GAGATGTCCG CACCAACGCG CAGCCCGGAC TCGGTAATGG CGCGCATTGC
     5941 GCCCAGCGCC ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC CCTCATTCAG
     6001 CATTTGCATG GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC GTTCCGCTAT
     6061 CGGCTGAATT TGATTGCGAG TGAGATATTT ATGCCAGCCA GCCAGACGCA GACGCGCCGA
     6121 GACAGAACTT AATGGGCCCG CTAACAGCGC GATTTGCTGG TGACCCAATG CGACCAGATG
     6181 CTCCACGCCC AGTCGCGTAC CGTCTTCATG GGAGAAAATA ATACTGTTGA TGGGTGTCTG
     6241 GTCAGAGACA TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA CAGCAATGGC
     6301 ATCCTGGTCA TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACGCGTTGCG CGAGAAGATT
     6361 GTGCACCGCC GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA CCACCACGCT
     6421 GGCACCCAGT TGATCGGCGC GAGATTTAAT CGCCGCGACA ATTTGCGACG GCGCGTGCAG
     6481 GGCCAGACTG GAGGTGGCAA CGCCAATCAG CAACGACTGT TTGCCCGCCA GTTGTTGTGC
     6541 CACGCGGTTG GGAATGTAAT TCAGCTCCGC CATCGCCGCT TCCACTTTTT CCCGCGTTTT
     6601 CGCAGAAACG TGGCTGGCCT GGTTCACCAC GCGGGAAACG GTCTGATAAG AGACACCGGC
     6661 ATACTCTGCG ACATCGTATA ACGTTACTGG TTTCACATTC ACCACCCTGA ATTGACTCTC
     6721 TTCCGGGCGC TATCATGCCA TACCGCGAAA GGTTTTGCGC
//