LOCUS       pHS0810_pcDNA3.1(+)_CMV 7038 bp ds-DNA     circular     04-JUL-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(850..948)
                     /label="bla promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        1217..1804
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1743..1761
                     /label="CMV For"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          1754..1774
                     /label="CMV forward primer"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        1848..1864
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          1848..1867
                     /label="T7 primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1867..1896
                     /label="HindIII GH"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1896..1901
                     /label="HindIII"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1908..3572
                     /label="Translation 1908-3572"
     misc_feature    1911..1937
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1944..3428
                     /label="OgeuIscB"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1944..3428
                     /label="Translation 1944-3428"
     misc_feature    2121..2123
                     /label="RuvC-I active"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2517..2519
                     /label="RuvC-II active site"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2679..2681
                     /label="HNH active site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2967..2969
                     /label="RuvC-III activ"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             3435..3482
                     /label="nucleoplasmin NLS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3489..3569
                     /label="3xHA"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     polyA_site      3623..3847
                     /label="BGH pA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      3893..4321
                     /label="f1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        4326..4695
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             4731..5525
                     /label="Neo(R)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     polyA_signal    5699..5829
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(6212..6882)
                     /label="pUC origin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(join(7027..7038,1..849))
                     /label="Amp(R)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
       61 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      121 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      181 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      241 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      301 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      361 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      421 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
      481 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
      541 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
      601 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
      661 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      721 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      781 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      841 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      901 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      961 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     1021 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     1081 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     1141 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     1201 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     1261 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     1321 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     1381 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     1441 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     1501 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     1561 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     1621 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     1681 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     1741 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     1801 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
     1861 tatagggaga cccaagctgg ctagcgttta aacttaagct tgccaccATG ggatcTCCAA
     1921 AAAAGAAACG GAAGGTGGGA TCtATGGCCG TGGTCTACGT GATCTCTAAG TCCGGCAAAC
     1981 CACTGATGCC CACCACCAGA TGCGGCCACG TGCGGATCTT GCTCAAGGAA GGCAAGGCTA
     2041 GAGTGGTGGA AAGAAAGCCC TTCACCATCC AGCTGACCTA CGAGTCTGCC GAGGAAACCC
     2101 AGCCTCTGGT GCTGGGCATT GACCCTGGCA GAACCAATAT CGGTATGAGC GTGGTGACCG
     2161 AGAGCGGAGA GAGCGTGTTC AACGCCCAAA TCGAGACAAG AAACAAGGAC GTGCCTAAGC
     2221 TGATGAAGGA CAGAAAGCAG TATAGGATGG CCCACAGAAG GCTGAAGCGG CGGTGCAAAA
     2281 GACGGCGGAG AGCCAAGGCT GCCGGCACCG CCTTCGAGGA GGGCGAAAAG CAAAGACTGC
     2341 TGCCTGGCTG CTTCAAGCCT ATTACATGCA AGAGCATCCG GAACAAGGAA GCCAGATTCA
     2401 ACAACCGCAA GCGGCCTGTG GGATGGCTGA CACCTACCGC CAATCACCTG CTGGTCACCC
     2461 ACCTGAACGT GGTTAAGAAG GTGCAGAAGA TCCTGCCCGT GGCCAAGGTG GTGCTGGAGC
     2521 TGAATCGGTT CAGCTTTATG GCCATGAACA ACCCCAAGGT TCAGAGATGG CAGTACCAGC
     2581 GGGGCCCTCT GTACGGCAAA GGCAGCGTGG AAGAGGCCGT GTCCATGCAG CAGGATGGAC
     2641 ACTGCCTGTT TTGTAAACAC GGCATCGACC ACTACCACCA CGTGGTCCCC AGAAGAAAGA
     2701 ACGGCAGCGA GACACTGGAA AACAGAGTGG GCCTTTGTGA AGAACACCAT AGACTGGTGC
     2761 ACACAGATAA GGAGTGGGAG GCCAACCTGG CCAGCAAGAA GTCTGGAATG AACAAGAAAT
     2821 ACCACGCCCT GTCTGTGCTG AACCAGATCA TCCCCTACCT GGCTGATCAG CTGGCCGATA
     2881 TGTTCCCCGG AAATTTCTGC GTGACAAGCG GCCAAGACAC CTATCTGTTT CGGGAAGAGC
     2941 ATGGCATCCC TAAGGACCAC TACCTGGACG CCTACTGCAT CGCCTGCAGC GCACTGACCG
     3001 ACGCCAAGAA GGTGAGCAGC CCTAAGGGCA GACCATACAT GGTGCACCAG TTCAGAAGGC
     3061 ATGATAGACA GGCCTGTCAC AAGGCCAATC TGAACCGGAG CTACTACATG GGCGGCAAGC
     3121 TCGTTGCCAC CAACCGGCAC AAAGCTATGG ACCAGAAAAC TGACAGCCTG GAAGAGTACA
     3181 GAGCCGCTCA CAGCGCCGCT GACGTGTCTA AACTGACCGT GAAGCACCCT TCTGCTCAGT
     3241 ACAAGGATAT GAGCAGAATC ATGCCTGGCA GCATCCTGGT GAGCGGCGAG GGCAAACTGT
     3301 TCACACTGAG CAGATCTGAG GGAAGAAACA AAGGCCAGGT GAACTACTTC GTGTCCACCG
     3361 AGGGCATCAA GTACTGGGCC AGAAAGTGCC AGTATCTGCG GAACAACGGC GGACTGCAGA
     3421 TCTACGTGGG ATCCaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa
     3481 agggatcTta cccatacgat gttccagatt acgcttaccc atacgatgtt ccagattacg
     3541 cttacccata cgatgttcca gattacgctT GAgcggccgc tcgagtctag agggcccgtt
     3601 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3661 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3721 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3781 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     3841 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     3901 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3961 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4021 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4081 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4141 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4201 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4261 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4321 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     4381 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     4441 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4501 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4561 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     4621 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     4681 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4741 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4801 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4861 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     4921 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4981 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5041 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5101 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5161 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5221 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     5281 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5341 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5401 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5461 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5521 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     5581 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5641 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     5701 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5761 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5821 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     5881 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     5941 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6001 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6061 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6121 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6181 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6241 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6301 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6361 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6421 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6481 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6541 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6601 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6661 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6721 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6781 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
     6841 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6901 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6961 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7021 tgacagttac caatgctt
//