LOCUS       pHS0812_hU6-BpiI-OgeuIs 3132 bp ds-DNA     circular     04-JUL-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    511..540
                     /label="Gibson homology"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    750..779
                     /label="Gibson Homology HindIII"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    751..755
                     /label="RBS"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    762..764
                     /label="Start codon"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        780..1028
                     /label="Human U6 Promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1029..1051
                     /label="BpiI Golden gate site for guide cloning"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     source          1031..1036
                     /label="pUC19"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1044..1049
                     /label="BbsI"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1052..1257
                     /label="OgeuIscB hRNA scaffold"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1258..1263
                     /label="PolyT terminator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1265..1294
                     /label="Gibson homology KpnI"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     source          complement(join(1677..3132,1..1676))
                     /label="pUC19"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     -35_signal      1807..1812
                     /label="bla promoter (counter-clockwise); TTCAAA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     -10_signal      1828..1833
                     /label="bla promoter (counter-clockwise); GAGACA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     sig_peptide     1877..1945
                     /label="bla"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1877..2737
                     /label="bla"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      join(2908..3132,1..364)
                     /label="pMB1 origin of replication"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     -35_signal      2908..2913
                     /label="RNAII promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     -10_signal      2929..2934
                     /label="RNAII promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_RNA        join(2944..3132,1..364)
                     /label="RNAII transcript (complementary strand)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_RNA        complement(2947..3054)
                     /label="RNAI transcript"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     -10_signal      complement(3063..3068)
                     /label="RNAI promoter (clockwise); GCTACA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     -35_signal      complement(3085..3090)
                     /label="RNAI promoter (clockwise); TTGAAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
       61 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      121 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      181 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      241 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      301 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      361 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      421 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      481 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      541 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      601 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      661 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      721 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccag
      781 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
      841 taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
      901 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
      961 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1021 gaaacaccAT GTCTTCCTGG GACGAAGACA AGGCTCTTCC AACTTTATGG TTGCGACCGT
     1081 AGGTTGAAAG AGCACAGGCT GAGACATTCG TAAGGCCGAA AGACCGGACG CACCCTGGGA
     1141 TTTCCCCAGT CCCCGGAACT GCATAGCGGA TGCCAGTTGA TGGAGCAATC TATCAGATAA
     1201 GCCAGGGGGA ACAATCACCT CTCTGTATCA GAGAGAGTTT TACAAAAGGA GGAACGGTTT
     1261 TTTggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     1321 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     1381 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     1441 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     1501 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     1561 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     1621 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     1681 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     1741 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1801 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1861 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1921 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1981 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     2041 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     2101 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     2161 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     2221 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     2281 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     2341 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     2401 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     2461 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     2521 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     2581 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2641 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2701 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2761 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2821 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2881 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2941 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3001 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3061 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3121 tctgctaatc ct
//