LOCUS       Exported                5781 bp DNA     circular SYN 28-NOV-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Jason Yu
  TITLE     Direct Submission
  JOURNAL   Exported Sep 8, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     complement(17..39)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     139..158
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     171..226
                     /label=Primer 1
     promoter        177..843
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase"
     primer_bind     177..202
                     /label=Gib_SacI_GAProm_f
     primer_bind     177..202
                     /label=p16_GAPprom_f
     primer_bind     177..198
                     /label=NotI_GAPprom_f
     primer_bind     687..706
                     /label=GPD fw 1
     primer_bind     734..753
                     /label=GPD fw 2
     primer_bind     761..784
                     /label=p426-sense(GPD_pr)
     primer_bind     complement(804..849)
                     /label=Primer 2
     primer_bind     complement(819..844)
                     /label=mCherry_GAPprom_r
     misc_feature    879..1588
                     /label=yomWasabi
     terminator      1610..1797
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1665..1689)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     1857..1887
                     /label=Primer 1
     misc_feature    1864..2367
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     1864..1884
                     /label=CEN/ARS_fwd
     primer_bind     complement(1864..1884)
                     /label=Gib_ARS_p16_rev
     primer_bind     complement(2363..2382)
                     /label=CEN/ARS_rev
     primer_bind     2363..2382
                     /label=Gib_ARS_p16_fwd
     primer_bind     2415..2433
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2471..2493)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2593..2612
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2757..2944
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             2945..3607
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     primer_bind     complement(3581..3613)
                     /label=Primer 2
     primer_bind     complement(3673..3692)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3674..3692)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3779..3796)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3950..4538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4030..4049)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4709..5569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5332..5351
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5570..5674)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5742..5760
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
       61 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
      121 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgtcat
      181 tatcaatact gccatttcaa agaatacgta aataattaat agtagtgatt ttcctaactt
      241 tatttagtca aaaaattagc cttttaattc tgctgtaacc cgtacatgcc caaaataggg
      301 ggcgggttac acagaatata taacatcgta ggtgtctggg tgaacagttt attcctggca
      361 tccactaaat ataatggagc ccgcttttta agctggcatc cagaaaaaaa aagaatccca
      421 gcaccaaaat attgttttct tcaccaacca tcagttcata ggtccattct cttagcgcaa
      481 ctacagagaa caggggcaca aacaggcaaa aaacgggcac aacctcaatg gagtgatgca
      541 acctgcctgg agtaaatgat gacacaaggc aattgaccca cgcatgtatc tatctcattt
      601 tcttacacct tctattacct tctgctctct ctgatttgga aaaagctgaa aaaaaaggtt
      661 gaaaccagtt ccctgaaatt attcccctac ttgactaata agtatataaa gacggtaggt
      721 attgattgta attctgtaaa tctatttctt aaacttctta aattctactt ttatagttag
      781 tctttttttt agttttaaaa caccaagaac ttagtttcga ataaacacac ataaacaaac
      841 aaagtcgacg gatcggtgac ggtgctggtt taattaacat ggtaagtaaa ggtgaagaga
      901 caactatggg ggtaatcaaa ccagacatga agatcaaact gaaaatggaa ggtaacgtta
      961 atggccacgc gtttgttatt gaaggtgaag gagaggggaa accttacgac ggaaccaaca
     1021 ctatcaattt ggaggttaag gaaggtgcac cattaccatt cagctatgac atcttaacaa
     1081 ctgcattttc atacggcaac agagctttta ccaagtaccc tgacgatata ccaaattact
     1141 tcaaacaatc ctttcctgaa ggttattcat gggagagaac aatgacgttt gaagataagg
     1201 gcattgtcaa ggtcaaatct gacatttcta tggaagagga ttcattcatc tacgagattc
     1261 acctcaaggg cgaaaacttc ccaccaaatg gtccagtgat gcaaaaggaa actactggat
     1321 gggatgcttc tacagaaagg atgtacgtga gagatggagt cttaaaaggt gatgttaaaa
     1381 tgaagttgct acttgaaggt ggtggccatc atagagttga tttcaaaaca atctacagag
     1441 ccaaaaaggc tgtaaaactt cctgattatc attttgtgga tcatcgtata gaaattctaa
     1501 accacgacaa agattacaat aaggtcactg tttatgaaat agcagttgcc agaaattcta
     1561 cagatggaat ggatgaattg tacaaataag gcgcgccact tctaaataag cgaatttctt
     1621 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta
     1681 aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt tcctgtaggt
     1741 caggttgctt tctcaggtat agtatgaggt cgctcttatt gaccacacct ctaccggcag
     1801 atccgctagg gataacaggg taatatagat ctgtttagct tgcctcgtcc ccgccgggtc
     1861 accgatcgct tgcctgtaac ttacacgcgc ctcgtatctt ttaatgatgg aataatttgg
     1921 gaatttactc tgtatttatt tatttttatg ttttgtattt ggattttaga aagtaaataa
     1981 agaaggtaga agagttacgg aatgaagaaa aaaaaataaa caaaggttta aaaaatttca
     2041 acaaaaagcg tactttacat atatatttat tagacaagaa aagcagatta aatagatata
     2101 cattcgatta acgataagta aaatgtaaaa tcacaggatt ttcgtgtgtg gtcttctaca
     2161 cagacaagat gaaacaattc ggcattaata cctgagagca ggaagagcaa gataaaaggt
     2221 agtatttgtt ggcgatcccc ctagagtctt ttacatcttc ggaaaacaaa aactattttt
     2281 tctttaattt ctttttttac tttctatttt taatttatat atttatatta aaaaatttaa
     2341 attataatta tttttatagc acgtgatgaa aaggacccta agaaaccatt attatcatga
     2401 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     2461 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     2521 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcgga cgtcggggct
     2581 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccataat tccgttttaa
     2641 gagcttggtg agcgctagga gtcactgcca ggtatcgttt gaacacggca ttagtcaggg
     2701 aagtcataac acagtccttt cccgcaattt tctttttcta ttactcttgg cctcctctag
     2761 tacactctat atttttttat gcctcggtaa tgattttcat tttttttttt ccacctagcg
     2821 gatgactctt tttttttctt agcgattggc attatcacat aatgaattat acattatata
     2881 aagtaatgtg atttcttcga agaatatact aaaaaatgag caggcaagat aaacgaaggc
     2941 aaagatgaca gagcagaaag ccctagtaaa gcgtattaca aatgaaacca agattcagat
     3001 tgcgatctct ttaaagggtg gtcccctagc gatagagcac tcgatcttcc cagaaaaaga
     3061 ggcagaagca gtagcagaac aggccacaca atcgcaagtg attaacgtcc acacaggtat
     3121 agggtttctg gaccatatga tacatgctct ggccaagcat tccggctggt cgctaatcgt
     3181 tgagtgcatt ggtgacttac acatagacga ccatcacacc actgaagact gcgggattgc
     3241 tctcggtcaa gcttttaaag aggccctagg ggccgtgcgt ggagtaaaaa ggtttggatc
     3301 aggatttgcg cctttggatg aggcactttc cagagcggtg gtagatcttt cgaacaggcc
     3361 gtacgcagtt gtcgaacttg gtttgcaaag ggagaaagta ggagatctct cttgcgagat
     3421 gatcccgcat tttcttgaaa gctttgcaga ggctagcaga attaccctcc acgttgattg
     3481 tctgcgaggc aagaatgatc atcaccgtag tgagagtgcg ttcaaggctc ttgcggttgc
     3541 cataagagaa gccacctcgc ccaatggtac caacgatgtt ccctccacca aaggtgttct
     3601 tatgtaggag ctcgaattca tcgatgatat cagatccact agtggcctat gcggccgcgg
     3661 atctgccggt ctccctatag tgagtcgtat taatttcgat aagccaggtt aacctgcatt
     3721 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     3781 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3841 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3901 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3961 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4021 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4081 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4141 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4201 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4261 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4321 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4381 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4441 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4501 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4561 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4621 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4681 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4741 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4801 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4861 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4921 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4981 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5041 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5101 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5161 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5221 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5281 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5341 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5401 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5461 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5521 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5581 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5641 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5701 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     5761 cctttcgtct cgcgcgtttc g
//
