LOCUS       Exported               14529 bp ds-DNA     linear   UNA 02-DEC-2021
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14529)
  AUTHORS   Angelo Batista
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 2, 2021 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14529
                     /organism="Escherichia coli"
                     /strain="BL21 (TaKaRa)"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     source          5291..5368
                     /organism="Escherichia coli"
                     /strain="BL21 (TaKaRa)"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     gene            209..285
                     /locus_tag="SR36_13695"
                     /label=SR36_13695
     tRNA            209..285
                     /locus_tag="SR36_13695"
                     /product="tRNA-Met"
                     /label=SR36_13695
                     /inference="COORDINATES:same species:tRNAscan-SE"
     gene            319..395
                     /locus_tag="SR36_13700"
                     /label=SR36_13700
     tRNA            319..395
                     /locus_tag="SR36_13700"
                     /product="tRNA-Met"
                     /label=SR36_13700
                     /inference="COORDINATES:same species:tRNAscan-SE"
     gene            429..505
                     /locus_tag="SR36_13705"
                     /label=SR36_13705
     tRNA            429..505
                     /locus_tag="SR36_13705"
                     /product="tRNA-Met"
                     /label=SR36_13705
                     /inference="COORDINATES:same species:tRNAscan-SE"
     gene            complement(579..1832)
                     /gene="amiC"
                     /locus_tag="SR36_13710"
                     /label=amiC
     CDS             complement(579..1832)
                     /codon_start=1
                     /gene="amiC"
                     /locus_tag="SR36_13710"
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /label=amiC
                     /note="hydrolyzes the bond between N-acetylmuramic acid
                     and amino acid residues in peptidoglycan; Derived by 
                     automated computational analysis using gene prediction 
                     method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11454.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPAS
                     SYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSARVG
                     QFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLEDYNK
                     GDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQIARRL
                     RSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFA
                     LSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKFGKAVL
                     NKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQQEVAESI
                     LAGIKAYFADGATLARRG"
     gene            2064..3395
                     /locus_tag="SR36_13715"
                     /label=SR36_13715
     CDS             2064..3395
                     /codon_start=1
                     /locus_tag="SR36_13715"
                     /product="N-acetylglutamate synthase"
                     /EC_number="2.3.1.1"
                     /label=SR36_13715
                     /note="catalyzes the formation of N-acetyl-L-glutamate
                     from L-glutamate and acetyl-CoA in arginine biosynthesis; 
                     Derived by automated computational analysis using gene 
                     prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11455.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSI
                     VNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAGTL
                     QLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDAIHR
                     QLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIV
                     SELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELF
                     SRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEIDKFTII
                     QRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQSGLSKLFV
                     LTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     primer_bind     3316..3335
                     /label=oAB276-recD_screen_F
     misc_feature    3415..3457
                     /label=H1
     misc_feature    3457..11801
                     /label=Whole RecBCDdeletion
     gene            complement(3457..5283)
                     /gene="recD"
                     /locus_tag="SR36_13720"
                     /label=recD
     CDS             complement(3457..5283)
                     /codon_start=1
                     /gene="recD"
                     /locus_tag="SR36_13720"
                     /product="exonuclease V subunit alpha"
                     /EC_number="3.1.11.5"
                     /label=recD
                     /note="helicase/nuclease; with RecBC catalyzes the 
                     exonucleolytic cleavage of DNA; RecD has 5' to 3' helicase 
                     activity; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11456.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSHD
                     AGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMILCG
                     DRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAVALT
                     RRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGKALRQ
                     LPLSDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDLPMMSR
                     LIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTGTHVPAG
                     TGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTDIEKRLLQ
                     SGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFGVAGLNERI
                     EQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGTRVWFAMPDG
                     NIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELVYTAVTRARRR
                     LSLYADERILSAAIATRTERRSGLAALFSSRE"
     primer_bind     5103..5122
                     /label=oAB280-recB_screen_F
     misc_feature    5246..5285
                     /label=H1
     gene            complement(5283..5376)
                     /gene="recB"
                     /locus_tag="SR36_13725"
                     /label=recB
     misc_feature    5286..5368
                     /label=SCAR
     gene            complement(5286..5290)
                     /gene="recD"
                     /locus_tag="SR36_13720"
                     /label=recD
     misc_feature    5369..8257
                     /label=pitrilysin
     gene            complement(5369..8257)
                     /locus_tag="SR36_13730"
                     /label=SR36_13730
     CDS             complement(5369..8257)
                     /codon_start=1
                     /locus_tag="SR36_13730"
                     /product="protease"
                     /label=SR36_13730
                     /note="degrades small peptides; Derived by automated 
                     computational analysis using gene prediction method: 
                     Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11458.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQYQ
                     AIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYPQA
                     DSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAERER
                     NAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQALKDFH
                     EKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKGIIIHY
                     VPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVEGISANS
                     DPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDELANVLDID
                     FRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMTPQNARIWY
                     ISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIPDDFSLIKSD
                     KKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQVMFALNDYLA
                     GLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGYFSYTATEDQLE
                     QAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSITLKEVLAYRDAL
                     KSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVDKKQSVIFEKAGNS
                     TDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLGYAVFAFPMSVGRQW
                     GMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQAVITQMLQAPQTLGE
                     EASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVVEPQGMAILSQISGSQN
                     GKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     misc_feature    5369..5407
                     /label=H2
     primer_bind     complement(5525..5544)
                     /label=oAB281-recB_screen_R
     gene            complement(8433..11801)
                     /gene="recC"
                     /locus_tag="SR36_13735"
                     /label=recC
     CDS             complement(8433..11801)
                     /codon_start=1
                     /gene="recC"
                     /locus_tag="SR36_13735"
                     /product="exonuclease V subunit gamma"
                     /EC_number="3.1.11.5"
                     /label=recC
                     /note="catalyzes ATP-dependent exonucleolytic cleavage in
                     either 5'- to 3'- or 3'- to 5'-direction to yield 
                     5'-phosphooligonucleotides; component of the RecBCD (Exo V)
                     helicase/nuclease complex that is essential for 
                     recombination; Derived by automated computational analysis 
                     using gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11459.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQW
                     LQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQLLE
                     REDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGEAQA
                     WQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISALPPV
                     YLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPLFRDSE
                     NAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNLLHNIQS
                     DILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDRLLAMLEE
                     DPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPVLEAFISLL
                     SLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDDDNVRELELP
                     ATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLASLLMQLNIWRR
                     GLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGLGAQYGDAVPLS
                     LLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMNDGVYPRQLAPLGF
                     DLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNSERFPSVLVQELID
                     YIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGERQSYAREWLPAASQ
                     AGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVNFRTEDSEIPDTEPFI
                     LEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGEIFWETQCQEMQQLADR
                     VIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLLSVAQGMQLWLEHLVYCA
                     SGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREGMSAPLLVLPESGGAWLKT
                     CYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDIWYQRLWRQLTPETMEAIVE
                     QSQRFLLPLFRFNQS"
     gene            complement(11814..12137)
                     /locus_tag="SR36_13740"
                     /label=SR36_13740
     CDS             complement(11814..12137)
                     /codon_start=1
                     /locus_tag="SR36_13740"
                     /product="hypothetical protein"
                     /label=SR36_13740
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11460.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASRN
                     QYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLHCP
                     NRQ"
     primer_bind     complement(11908..11927)
                     /label=oAB277-recC_screen_R
     gene            complement(12122..12529)
                     /locus_tag="SR36_13745"
                     /label=SR36_13745
     CDS             complement(12122..12529)
                     /codon_start=1
                     /locus_tag="SR36_13745"
                     /product="hypothetical protein"
                     /label=SR36_13745
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11461.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQSL
                     RRQAIVQSALAWGKMHSWQTQPAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSLWR
                     TGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(12526..13089)
                     /locus_tag="SR36_13750"
                     /label=SR36_13750
     CDS             complement(12526..13089)
                     /codon_start=1
                     /locus_tag="SR36_13750"
                     /product="hypothetical protein"
                     /label=SR36_13750
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11462.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTGTRKL
                     ALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREPVK
                     ESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVLTVN
                     MRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(13080..13550)
                     /locus_tag="SR36_13755"
                     /label=SR36_13755
     CDS             complement(13080..13550)
                     /codon_start=1
                     /locus_tag="SR36_13755"
                     /product="hypothetical protein"
                     /label=SR36_13755
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11463.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTASQ
                     ARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEVEM
                     SDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(13735..14529)
                     /locus_tag="SR36_13760"
                     /label=SR36_13760
     CDS             complement(13735..14529)
                     /codon_start=1
                     /locus_tag="SR36_13760"
                     /product="thymidylate synthase"
                     /label=SR36_13760
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11464.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPLV
                     TTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWPTP
                     DGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLSCQL
                     YQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHLQLSR
                     EPRPLPKLIIKRKPESIFDYSFEDFEIEGYDPHPGIKAPVAI"
ORIGIN
        1 tgttctcttc ttcaggttaa aaaataaggc gcaacgaaga taacaaaccg ccgcgtttaa
       61 tgaaatgagc agcctctccc tgacgcaaat tttgcacaaa aaataggctt tagtgatttg
      121 tttttgttca aaatcatgcc aaatccgtga tcggggtaaa aaaaaggttg catgaaaacg
      181 cgagcggagt atagtgcgca tccacggacg cggggtggag cagcctggta gctcgtcggg
      241 ctcataaccc gaaggtcgtc ggttcaaatc cggcccccgc aaccaattaa aatttgatga
      301 agtaaagcag tacggtgacg cggggtggag cagcctggta gctcgtcggg ctcataaccc
      361 gaaggtcgtc ggttcaaatc cggcccccgc aaccaatcaa atttgatgaa gtaaaagcag
      421 tacggtgacg cggggtggag cagcctggta gctcgtcggg ctcataaccc gaaggtcgtc
      481 ggttcaaatc cggcccccgc aaccaattat tgaacaccct aacgggtgtt tttttgtttc
      541 tggtctccca taaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtc
      601 gccccatcgg caaaatacgc tttaatcccc gcaagaatag actccgcaac ttcctgctgg
      661 aaagtcgccg ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc
      721 agaatggagg gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt
      781 ttatgcagct tgttgatttt accgagctta ttcagtaccg ctttaccaaa cttcaggctg
      841 tcggcaatgg tcagcgactg taccatatcg aacatggtgt ggtcgacata gcggtcaccg
      901 cttttgctca cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc
      961 gcagtactgg ttgcaccttt ggttgagagc gcaaacacag aggaaccgct cggctgacga
     1021 ctggtaaagg cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgggctttt
     1081 gctacgcgca cttgcaatgg aatgaagatg tcttcattgc gcgtcatgta caccttcata
     1141 ttgccctctt tctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacatctttt
     1201 tcgcgcgttt tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc
     1261 ataatgacaa tcggacgatc gcgccctgct ttacccggtt gtggaccact ttgcgctggc
     1321 ggcacctgct tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc
     1381 tgcatatcct gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg
     1441 accggcgcaa gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt
     1501 acggtttgcg ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga
     1561 atttgtgccg ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc
     1621 acgcgttccg ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc
     1681 gtcacacggg tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca
     1741 gccaggctga cctgacttac gctcaatagc cacatggcgc ccgcgccttg cagtaaacga
     1801 cgacggctga ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc
     1861 tgtgtaacaa gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc
     1921 atctaataaa cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc
     1981 tcgcataaaa tttacacttg caccctgcga aaaaacagaa taaaaataca ctaatttcga
     2041 ataatcatgc aaagaggtgt gccgtggtaa aggaacgtaa aaccgagttg gtcgagggat
     2101 tccgccattc ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg
     2161 gcggtgaagc cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc
     2221 acagcctcgg catccgtctg gtggtagtct atggcgcacg tccgcagatc gacgcaaatc
     2281 tggctgcgca tcaccacgaa ccgctatatc acaagaacat acgtgtgacc gacgccaaaa
     2341 cactggaact ggtgaagcag gctgcaggaa cattgcaact ggatattact gctcgcctgt
     2401 cgatgagtct caataacacg ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt
     2461 ttattattgc ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta
     2521 tccggcggat tgatgaagac gcgatccatc gtcaactgga cagcggtgca atagtgctaa
     2581 tggggccggt cgctgtttca gtcactggcg agagctttaa cctgacctcg gaagagattg
     2641 ccactcaact ggccatcaaa ctgaaagctg agaagatgat tggtttttgc tcttcccagg
     2701 gcgtcactaa tgacgacggt gatattgtct cagaactttt ccctaacgaa gcgcaagcgc
     2761 gggtagaagc ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg
     2821 gcgcagtgaa agcctgccgc agcggcgtgc gtcgctgtca tttaatcagt tatcaggaag
     2881 atggcgcgct gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg
     2941 aaagcgccga gcagattcgt cgcgcaacaa tcaacgatat cggcggtatt ctggagttga
     3001 ttcgcccgct ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg
     3061 aaatcgacaa attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct
     3121 atccgttccc ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc
     3181 gcagttcatc acggggcgag gttctgcttg aacgcattgc cgctcaggcg aagcagagcg
     3241 gcttaagcaa attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat
     3301 ttaccccggt ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc
     3361 gtaaatccaa agtgttgatg gcggatttag ggtaagcgac gaaaatcgcc ggatgcgaca
     3421 tgcgtaacac tcgtgcgtcg catccggcaa ttacgtttat tcccgcgaac taaacaacgc
     3481 cgccagacca ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tacgctcatc
     3541 ggcatacagc gacagacggc gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt
     3601 tactaccggc gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg
     3661 cgatttatgt accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac
     3721 agacttaata ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag
     3781 cgcaataccg atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcatcac
     3841 cggtcggcct tcgtaccaac gagagtgcgg atgacgatga attttgcgct tctgttgcat
     3901 aaactgctca attcgctcat tcagtccagc cacgccaaac ggcccttccc gcagggcgca
     3961 caaaagctgg tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag
     4021 cagatccaga taacgtccgt aacccgcaag agcttcctca agcatcgcaa tataatcttc
     4081 gccgctctgt aaaagtcgtt tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac
     4141 tgccgtttta tcaccacgat tgatcgccgc agctaactga ccaatgccag aatcgctgcc
     4201 gaaacgatag cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc
     4261 ggttcctgcc ggaacgtgag tccccgtcag gcggcttagc tgcctggcac gctcggcggt
     4321 aaagcccgcg ttggcataag cgcagatatc acccagcaca gccccagcct caaccgaggc
     4381 cagttgatca cgatcgccga gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag
     4441 tctcgacatc ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg
     4501 cagcgggtta ccggcatgat gacgtaaacg ctggctaccc ggctgcgcac ccagcaatcg
     4561 gtgcaaagtg ctggcatctt ccggaatgcg tttcttttgt tcatcgctca gcggtaactg
     4621 tcgcaaagcc ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc
     4681 cagacggata cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gcaacttcgc
     4741 cacggtggtc gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag
     4801 cgccactgcc gccgcaactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc
     4861 cagggtttgc gccagtagag cttcatcaac ctcgatggca tgattcacct cattgaagaa
     4921 gcgcgctacc gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag
     4981 gatcatcggc gtgggttcat ctccccggct gaccgcttgt gaagccagca agcattcttc
     5041 ccaattttgt agctcaccga tttcactgac acaggtcgcc aacagcggat gcgacgcctc
     5101 gttattttcc agtcgtgaaa gcggtaaaca gacatgccct tccccggcgt cgtggcttaa
     5161 cagggctgcc gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaattg
     5221 cacatccagc gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt
     5281 cattaattcc ggggatccgt cgacctgcag ttcgaagttc ctattctcta gaaagtatag
     5341 gaacttcgaa gcagctccag cctacacatc actcattctt ttcactcatc aggggcattg
     5401 tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc ttcagggtgt acatattcgg
     5461 ctttcccgtt ctggctgccg gaaatctgcg acagaatagc catgccttgc ggctcgacca
     5521 ccgcctgatg gaagaaatca gcaagttttt gcggcgtcag cagttttatc tgggccacga
     5581 ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa atctttactt aacttcgatg
     5641 cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt aattaccgcc tgctggattt
     5701 gcgcaaactc atctggcttc atcgctcgca attttgcctc tgcggttggg aaaaacgcct
     5761 tgtaacgctc ccacaagaat gaaggctgtt tatcattgct ttgcaaaagg aagcccatgc
     5821 cccactgacg ccccacgctc attggaaacg caaacacggc atagcccaat tgttcttcgg
     5881 tacgcaactg attgtagaac cacggctgta cgatctgccc caacagagag ctataggctg
     5941 agctggtgta ttcatcgtag ccagtcggta caaataccgc tgccagtgcg gagtcggtgc
     6001 tgttaccggc tttttcaaag atgacggatt gttttttatc gaccactaca tctttgtttc
     6061 gacaccactc tgaaccatca gcgcccaact gtttttgcac atcgcgtgcc agcgttgttg
     6121 cctgggcctc ggtcatgttg ccgataacca taaactctgg tcgagcccct gattttaagg
     6181 cgtcgcgata ggccagcacc tctttcaacg taatggaggg caaaatttta cgccgttcat
     6241 ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg cataatcgcc tgctcaaacg
     6301 ctttaccctt ttctgcggaa tccatcatct ggttatacca ggacttcgcc tgctcaagct
     6361 gatcttccgt ggcggtatag ctaaagtagc cttccagcaa tgcctggaac agctgcggca
     6421 gacgctgggt gtaaccatta gcattaacca taaggccgtt gttagcgttg gtggaaaaac
     6481 ttatgccacc aaccgacgcc tggttgctta actgatcaag cgccagccct gcgagataat
     6541 cattgagcgc aaacatcacc tgattgcggg cgctgtccat ggctttcgga ttacgcaaaa
     6601 tcaggctgac atcggctttt ggctcgctgg caaaataacg gcttggcgca tacactacgc
     6661 gcagattcga ctcatcaaca atcagctctg gatggtcata tttcttgtct gacttaatca
     6721 gcgagaaatc gtcaggaata taagggttaa gctctggcaa agagagcgca atgtcggcgg
     6781 cttttttctg ccagtcggcg aaagtttgtg cgctgatttt atcgacctga tacggcgcat
     6841 cgacaaagta agccgttttg ttgtgcggct ctttcgggct gatataccag atacgcgcat
     6901 tctgcggcgt catcatcgcc agacgttcct ttactgcttt agcatcgtac cgatcggcaa
     6961 tattgactgc atccagcgta tgctcaacag gaacgcgaat catggtatct gccagccatt
     7021 cgacgtaatc catatcacgg gtgattgacg gataacggaa gtcgatatcc agcacattcg
     7081 ccagttcatc gaagtattgt ttatcgatcc ctttttcacg taacaaattg aggtagctaa
     7141 aaatagccgc cacaacctga tcgcgattcg ccagaccttt atcggttaaa gacgcagaga
     7201 tcgctaatac gccgctgttg ccgttgacga taggatcgga gttggcgcta atgccctcaa
     7261 ctaatccctg cttttgcagc cagtcggaaa gtgtacctgg gctgcgatta ccaatcagat
     7321 aggtaatcaa ttcatcggtt ttgctacgga atttcgccga gttgttatca atacgaaact
     7381 ctacgcgcaa cactttacgc ggcagcgccg ggacgtaatg aatgataatg cccttttgcg
     7441 cgtcggtgac taccggcacg gtgatttccg gttttttgct ctctttgttc ggcacgcgac
     7501 caaaggtgtc cgccgccatt tttgccaact ccggcagcgg tttattactg taaataaccg
     7561 ccttcatcaa attggcggaa tagtacttct cgtggaaatc tttcagcgcc tgctgcaccg
     7621 gattaccagg tttgtcgctt aaagtttcga ggttaccacc agaaaacttt gaaccggggt
     7681 gtgccgggtt aatggtttct gcgctgacct gtgccatgcg catcccgtca cgcgtacgcg
     7741 ccatggttaa ttcagcgttc accgcattac gctcacgttc ggcatatttc ttgtcgagca
     7801 aaggttcagc aatagcatcg gccaggcggt ctaccgcacc aggcaaggcg tcgttctcaa
     7861 cttccagata gaaagccgtg cgatacggcg cagtgctggc attgtgacta ccgccgtgca
     7921 ttttgagata ttcggccaga ctgtcagcct gcgggtactt tttcgacccc atcagactca
     7981 tatgttcaag gtaatgtgcc agcccctggt acgcctcggg atcttccagc gacccaacgg
     8041 gcaccaccag cgccgagagc gatttaaccg cctgcggatc agaaaccagc aagaccacca
     8101 taccgttatc cagacgtata gcctgatact ggcggttatc tttatcactt ttacggatgg
     8161 tttcctgaat cggctgccat cccgtttctg cctgacttaa gggtgcccaa agggcaacaa
     8221 acaacaataa tgctttgaac caggtgctgc ggggcattca cggacctcat aatcaacttt
     8281 attttctggc cagattcaac aggttaacgc aaatcatctg ccagaattta atcttgtgct
     8341 gcacgagtca gcctatgtct atatatccat cagtccgtga ctggtgcgca tcataatgta
     8401 agcgaatgaa ttgcgcaatt tttatacagc actcatgact gattaaagcg aaacagcggt
     8461 aacaggaaac gttgcgactg ttcaacgatg gcctccattg tctctggtgt taattgccgc
     8521 cagagccgtt gataccagat atcatcacct tcgccacgca ccatcatgtt gccttcgtaa
     8581 gcctgaagga atttcgtacg ggctttttgc aacgtggaat cgtcatccag catggcatcg
     8641 ttttgcgcgt cataacaggt ttttagccac gcgccgccac tttcaggtaa caccagcaat
     8701 ggcgcggaca ttccttcacg atacccctca atcagttgtg agaggtaatg caaagcctgt
     8761 tcggctgcaa gcggcggaaa acgccactca ccgtctttgc gtagaaaaag gcgactttcg
     8821 ccattaccac cgctggcaca gtagacaagg tgttccagcc aaagttgcat tccctgcgcc
     8881 acacttaata aagagggacg ccagcgcaac aggccatccg gctgcacctg cggcaaccag
     8941 ccagttatct gcacaccgtt gcaggcgaga tcaatttcca tactctgccc cggctggcga
     9001 caggcaatga ctctgtcggc aagctgctgc atctcctggc actgtgtttc ccagaaaatt
     9061 tcaccaaaag cgccatacgg taaatcccct gccgcccgga agcggcggaa caagcgttcg
     9121 gcatcatcct gctcaaccag tgcattcaat aactgctgat tgatttgata acggctaagt
     9181 ccttccagaa taaatggctc ggtgtctggg atttcgctgt cttcagtacg gaagttcacc
     9241 tgcaaacgca tctggaagaa cgcccgcacc ggatgtgccc agaatcgttg tagcgtttcc
     9301 agcggcacgg tttccggtaa ggtaaacggc agcggctgaa caaattcaga atgtgcttta
     9361 ccagcctggc tggccgcagg tagccattca cgagcatagc tttgtcgttc gcctggctgg
     9421 tagttttgtg gatcaaacgg catccgggta tggaggcaag taagatgcgc ttttaccctt
     9481 gcctcgcttt catcacagtt gagcgcttca tcgcccggta gataatgact ttgcccgatg
     9541 tagtcgatca gttcctgcac cagtaccgac gggaaacgtt cactgttatc ctgaatggaa
     9601 cgcccgatat agctgatata gagtttttgc tgcgcggaaa ttaacgcttc caggaacagg
     9661 tagcggtcgt catcgcgacg gctacggtcg ccacgcttcg gtttctggct catcaggtca
     9721 aagcccaatg gcgcaagctg acgtggataa acgccgtcgt tcattcccag caggcaaacc
     9781 actttgaacg gaattgaacg cattggcatc agagtacaaa tgttaaccgg tccggcgaga
     9841 aaacgctggc tgatacgttc ttgatccaga cgctgcgcca gttcatcacg caatagtgac
     9901 agcggcaccg cgtcgccata ctgcgcacct aaaccttcgg cgataatcgc ctgccattgt
     9961 tgttcgatca gcgtcatcgc cgcttcggtt tccgcatccg gcaggaagaa ggcgttgagc
    10021 atatcgcgac aaaccggcaa ccactcttcc agcggacgct cctgtgccag cccgcgacgc
    10081 cagatgttta gctgcattag cagtgaagcc agatgcccca ccagttctgc aattaagccg
    10141 ctcgattcat cataaggtag aaccgattgc cactcgccct gcgcgctctc catcgcgtag
    10201 cccagcaaca tacgcgtcag gccaaatcgc caggtgtgtt gtccggtggc ggggagttcc
    10261 agctcgcgaa cgttgtcgtc atctatcccc caacgaatgc cggattcgtt gacccactgg
    10321 cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg ccgccagcac cggcacatcc
    10381 agcagcgcca gcacatcctc tgacacaaag cgactgtcag gcagtgataa caggctgata
    10441 aacgcttcca gtaccggatg tgactgccgc gcacgacggt cggaaatggc gtaaggcagg
    10501 taacgatccg caggtgcgct accaaacact gcctgaataa acggactgta gctgtcgata
    10561 tcagccacca tcacgatgat gtcgcgcgga gtaagtgtcg ggtcttcctc cagcatcgcc
    10621 agcaagcgat cgtgtaaaac ttcaacttca cgctgcgggc tatggcaaac gtggaatgtg
    10681 atactgctat ccagtggatc aagcgggcgt ttgttatcgc tacgggaaaa ctcttcgatg
    10741 ttcacacccg caacggcgcg gttttccagt tccagaatgt cagactgaat gttatgcagc
    10801 aggttatctg gcgtgacatc gacaaaggcg tccagctcct ggctgctctc caggtcagaa
    10861 aggagataaa tgtagtcgcg cccaagctta ccccatgaag ccagcagcgg gttgccgaca
    10921 tcctgttcac catcgctgtt aaagagctgc ccggcatttt cgctatcacg aaatagcggc
    10981 aattcgcgat cttcaaaact gtgtcgacgc tgacgggtca gcagtttcgc cagataagca
    11041 ggatctttaa tatcgcccca gtaataacgg caggggttgg taaacaggag atggatttca
    11101 atatgtttac ccagcgcctg gagcgcctgg agataaacag gcggtaacgc ggaaataccg
    11161 catataaaga cgcgcgaagg taaccccggc gggcaggtcg tcgcggactc cagcgtttcg
    11221 ataaagcgct gatagagatt ggcgcggtgc cagcgcggtt gtccgagttc atgggtatat
    11281 tccaccagcg ccttccacaa cggcgcttgc caggcctgtg cttctcccag cccttcaacc
    11341 aaatgtcctg tttcccattg tgccagccac tccgggcgat agaccagata ctggtcaaac
    11401 aggtccgccg cttttgagga aagctggaac agttttcgct tgtcgctatc gtcagtcaga
    11461 taatgccgca acagggtaaa gtcttcgcgc tccagcaatt gcggcagcag agtcatcagt
    11521 ttccagctca tgctctgttt gttaaaggcg ctctctttgg ggatttccgg taacacccgg
    11581 acgaacatat cccagataaa gctcgctggc agcggaaaat caatgtttgc cgcaatacca
    11641 aacttttgcg acagggtcat ttgcagccac tgcgccatac cggtactttg caccagaatc
    11701 atctctggtt cgaaaggatc gtccagccgt tcgcgttcga caataaactc catcaacgct
    11761 tccagcacgt ccagacgatt ggaatggtag acccttaaca tagcggctcc tgactactga
    11821 cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc ccccgggtga aactagcgta
    11881 acgctgatgc tgacacatcc cgcctgcgat gtctgcattc ggttgacctg ccagttggca
    11941 ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccagccat gccgccagag ctgttggtac
    12001 tggtttctgc tggcaagact gttcattaat gttcgctgga aacccgataa cgcagtgaca
    12061 atcatcacca tcaacaccat cgccaacatt acctccggca ggctaaagcc ttgttgattc
    12121 ttcagggaag ctgacataac gccctctctt tcagcggaca aaaatcgctc cagccgcgtg
    12181 gcgaaaaaac aatgtttcca tcaatgactt cgcctgttcg ccacaacgaa acgccttcat
    12241 aaccggcaat caataaggct tcattatctg ccagtaaacg caaacaaacc tgggcatcgg
    12301 tttcagcgta ctgcgagcac tgaactgctg gctgcgtctg ccaggagtgc atttttcccc
    12361 acgccagcgc cgactgaacg atggcctggc ggcgcaatga ctgactttcc atgctcacgc
    12421 gagaggcaaa actgcgatcc tgctgactca ttccttgcaa tagcaagcta cccaaaacca
    12481 gcagcatcag gaccagagcc agtgacgaaa cacctttttc gcggttcaca ggttgaatcc
    12541 tgtcacgcta tagctggcat ccaccacggt ttgcggttca gacttactgg cagcacgcat
    12601 attaaccgtc aacaccggcg agaagccgct gacatcctga cgtacgacct gaaaagtgtc
    12661 gataatgatg gcatccggat tagtgacttt atcccagccc ttaccttcac aggatgtcgc
    12721 accgcgtagc gtttccagca catgctcctt cagacgaaat ccaatctggt cggactcttt
    12781 taccggttcg cgatcccaga taccgttact gttcgcatcc cactgcacaa tgatacagtc
    12841 accctgtccg acaatttcca gcccttcgcc ggtacagctg ccatgacaat aacccgccct
    12901 ctggagatgc ttcgcgacgg taaatacccg cagccagatt tcatcttcca gcgccagctt
    12961 acgggtgccc gttaaacttt cacgctgtaa cgcaggcaga aagcgtgccg ctcccagcaa
    13021 caatacgcta ctgatcgcca tagcaatcaa cacttccagc agagaaaaac cttgctcttt
    13081 tacaggcatc cttctgtttc tccttgctga caaagccgga gtcttcccca cggcgaaacc
    13141 accagccacc actcgcccgt tgagtttttg aagcgaatat gcccggccca tgcggtattg
    13201 cgcaggccaa agaaagcaag cgaaggtgtc aggtcgctca tttcgacttc gggccagcgt
    13261 ggcacaaaga ccaatggtga actgccatga caggtattcg ccccagcaac ggaactcaca
    13321 aggcaccata acgtcccctc tctgataaca ctgatactgt ggtcgcggtt atgccagttg
    13381 gcatcttcac gtaaatagag caaatagtcc cgcgcctggc tggcggtttg ccatagccgt
    13441 tgcgactgct gccagtattg ccagccatag agtccacttg cgcttagcat gaccagaatc
    13501 agcatcgcga ccagcgtttc aatcagcgta taaccacgtt gtgttttcat gccggcagta
    13561 tggagcgagg agaaaaaaag acgagggcca gtttctattt cttcggcgca tcttccggac
    13621 tatttacgcc gttgcaggac attgcaaaat ttcgggaagg cgtctcgaag aatttaacgg
    13681 agggtaaaaa aaaccgacgc acactggcgt cggctctggc aggatgtttc gtaattagat
    13741 agccaccggc gctttaatgc ccggatgcgg atcgtagcct tcaatctcaa agtcttcgaa
    13801 actgtagtcg aagatggatt cgggtttacg tttgataatc aacttcggca gcggacgcgg
    13861 ttcgcggctt aattgcagat gagtttgatc catatgattg ctgtacaggt gcgtatcgcc
    13921 accggtccag acaaaatcac ccacttccag atcgcactgc tgcgccatca tatgcaccag
    13981 taacgcgtag ctggcaatgt tgaacggcag gccgaggaag acgtcacagg agcgctgata
    14041 aagctggcaa gagagtttgc cgtctgccac atagaactgg aagaatgcat ggcacggtgc
    14101 cagcgccatt ttatccagtt cgcctacgtt ccacgctgaa acaataatgc ggcgcgaatc
    14161 cgggtcgttt ttcagctggt tcagtaccgt agtgatctgg tcaatatgac gaccatctgg
    14221 cgttggccag gcgcgccact gtttaccata cactggcccg aggtcgccgt tttcatcggc
    14281 ccattcgtcc cagatggtga cattgttttc gtgtagataa gcaatgttag tgtcgccctg
    14341 caggaaccac agcagttcgt ggatgatgga acgcaggtgg caacgtttag ttgtcaccag
    14401 cgggaatcca tcctgcaggt taaaacgcat ctgatgacca aaaatggaaa gcgttccggt
    14461 tccggtacgg tcgtttttct gtgtgccttc gtcgagcact ttttgcatca gttctaaata
    14521 ctgtttcat
//
