LOCUS       Exported                6269 bp ds-DNA     linear   UNA 02-DEC-2021
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Angelo Batista
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 2, 2021 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Escherichia coli"
                     /strain="BL21 (TaKaRa)"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     source          3464..3541
                     /organism="Escherichia coli"
                     /strain="BL21 (TaKaRa)"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     gene            209..285
                     /locus_tag="SR36_13695"
                     /label=SR36_13695
     tRNA            209..285
                     /locus_tag="SR36_13695"
                     /product="tRNA-Met"
                     /label=SR36_13695
                     /inference="COORDINATES:same species:tRNAscan-SE"
     gene            319..395
                     /locus_tag="SR36_13700"
                     /label=SR36_13700
     tRNA            319..395
                     /locus_tag="SR36_13700"
                     /product="tRNA-Met"
                     /label=SR36_13700
                     /inference="COORDINATES:same species:tRNAscan-SE"
     gene            429..505
                     /locus_tag="SR36_13705"
                     /label=SR36_13705
     tRNA            429..505
                     /locus_tag="SR36_13705"
                     /product="tRNA-Met"
                     /label=SR36_13705
                     /inference="COORDINATES:same species:tRNAscan-SE"
     gene            complement(579..1832)
                     /gene="amiC"
                     /locus_tag="SR36_13710"
                     /label=amiC
     CDS             complement(579..1832)
                     /codon_start=1
                     /gene="amiC"
                     /locus_tag="SR36_13710"
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /label=amiC
                     /note="hydrolyzes the bond between N-acetylmuramic acid
                     and amino acid residues in peptidoglycan; Derived by 
                     automated computational analysis using gene prediction 
                     method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11454.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPAS
                     SYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSARVG
                     QFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLEDYNK
                     GDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQIARRL
                     RSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFA
                     LSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKFGKAVL
                     NKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQQEVAESI
                     LAGIKAYFADGATLARRG"
     gene            2064..3395
                     /locus_tag="SR36_13715"
                     /label=SR36_13715
     CDS             2064..3395
                     /codon_start=1
                     /locus_tag="SR36_13715"
                     /product="N-acetylglutamate synthase"
                     /EC_number="2.3.1.1"
                     /label=SR36_13715
                     /note="catalyzes the formation of N-acetyl-L-glutamate
                     from L-glutamate and acetyl-CoA in arginine biosynthesis; 
                     Derived by automated computational analysis using gene 
                     prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11455.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSI
                     VNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAGTL
                     QLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDAIHR
                     QLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIV
                     SELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELF
                     SRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEIDKFTII
                     QRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQSGLSKLFV
                     LTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     primer_bind     3316..3335
                     /label=oAB276-recD_screen_F
     misc_feature    3415..3457
                     /label=H3
     gene            complement(3457..3463)
                     /gene="recD"
                     /locus_tag="SR36_13720"
                     /label=recD
     misc_feature    3458..3541
                     /label=SCAR
     misc_feature    3542..3582
                     /label=H4
     gene            complement(3554..3877)
                     /locus_tag="SR36_13740"
                     /label=SR36_13740
     CDS             complement(3554..3877)
                     /codon_start=1
                     /locus_tag="SR36_13740"
                     /product="hypothetical protein"
                     /label=SR36_13740
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11460.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASRN
                     QYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLHCP
                     NRQ"
     primer_bind     complement(3648..3667)
                     /label=oAB277-recC_screen_R
     gene            complement(3862..4269)
                     /locus_tag="SR36_13745"
                     /label=SR36_13745
     CDS             complement(3862..4269)
                     /codon_start=1
                     /locus_tag="SR36_13745"
                     /product="hypothetical protein"
                     /label=SR36_13745
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11461.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQSL
                     RRQAIVQSALAWGKMHSWQTQPAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSLWR
                     TGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(4266..4829)
                     /locus_tag="SR36_13750"
                     /label=SR36_13750
     CDS             complement(4266..4829)
                     /codon_start=1
                     /locus_tag="SR36_13750"
                     /product="hypothetical protein"
                     /label=SR36_13750
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11462.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTGTRKL
                     ALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREPVK
                     ESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVLTVN
                     MRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(4820..5290)
                     /locus_tag="SR36_13755"
                     /label=SR36_13755
     CDS             complement(4820..5290)
                     /codon_start=1
                     /locus_tag="SR36_13755"
                     /product="hypothetical protein"
                     /label=SR36_13755
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11463.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTASQ
                     ARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEVEM
                     SDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(5475..6269)
                     /locus_tag="SR36_13760"
                     /label=SR36_13760
     CDS             complement(5475..6269)
                     /codon_start=1
                     /locus_tag="SR36_13760"
                     /product="thymidylate synthase"
                     /label=SR36_13760
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AJH11464.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPLV
                     TTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWPTP
                     DGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLSCQL
                     YQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHLQLSR
                     EPRPLPKLIIKRKPESIFDYSFEDFEIEGYDPHPGIKAPVAI"
ORIGIN
        1 tgttctcttc ttcaggttaa aaaataaggc gcaacgaaga taacaaaccg ccgcgtttaa
       61 tgaaatgagc agcctctccc tgacgcaaat tttgcacaaa aaataggctt tagtgatttg
      121 tttttgttca aaatcatgcc aaatccgtga tcggggtaaa aaaaaggttg catgaaaacg
      181 cgagcggagt atagtgcgca tccacggacg cggggtggag cagcctggta gctcgtcggg
      241 ctcataaccc gaaggtcgtc ggttcaaatc cggcccccgc aaccaattaa aatttgatga
      301 agtaaagcag tacggtgacg cggggtggag cagcctggta gctcgtcggg ctcataaccc
      361 gaaggtcgtc ggttcaaatc cggcccccgc aaccaatcaa atttgatgaa gtaaaagcag
      421 tacggtgacg cggggtggag cagcctggta gctcgtcggg ctcataaccc gaaggtcgtc
      481 ggttcaaatc cggcccccgc aaccaattat tgaacaccct aacgggtgtt tttttgtttc
      541 tggtctccca taaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtc
      601 gccccatcgg caaaatacgc tttaatcccc gcaagaatag actccgcaac ttcctgctgg
      661 aaagtcgccg ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc
      721 agaatggagg gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt
      781 ttatgcagct tgttgatttt accgagctta ttcagtaccg ctttaccaaa cttcaggctg
      841 tcggcaatgg tcagcgactg taccatatcg aacatggtgt ggtcgacata gcggtcaccg
      901 cttttgctca cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc
      961 gcagtactgg ttgcaccttt ggttgagagc gcaaacacag aggaaccgct cggctgacga
     1021 ctggtaaagg cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgggctttt
     1081 gctacgcgca cttgcaatgg aatgaagatg tcttcattgc gcgtcatgta caccttcata
     1141 ttgccctctt tctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacatctttt
     1201 tcgcgcgttt tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc
     1261 ataatgacaa tcggacgatc gcgccctgct ttacccggtt gtggaccact ttgcgctggc
     1321 ggcacctgct tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc
     1381 tgcatatcct gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg
     1441 accggcgcaa gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt
     1501 acggtttgcg ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga
     1561 atttgtgccg ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc
     1621 acgcgttccg ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc
     1681 gtcacacggg tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca
     1741 gccaggctga cctgacttac gctcaatagc cacatggcgc ccgcgccttg cagtaaacga
     1801 cgacggctga ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc
     1861 tgtgtaacaa gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc
     1921 atctaataaa cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc
     1981 tcgcataaaa tttacacttg caccctgcga aaaaacagaa taaaaataca ctaatttcga
     2041 ataatcatgc aaagaggtgt gccgtggtaa aggaacgtaa aaccgagttg gtcgagggat
     2101 tccgccattc ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg
     2161 gcggtgaagc cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc
     2221 acagcctcgg catccgtctg gtggtagtct atggcgcacg tccgcagatc gacgcaaatc
     2281 tggctgcgca tcaccacgaa ccgctatatc acaagaacat acgtgtgacc gacgccaaaa
     2341 cactggaact ggtgaagcag gctgcaggaa cattgcaact ggatattact gctcgcctgt
     2401 cgatgagtct caataacacg ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt
     2461 ttattattgc ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta
     2521 tccggcggat tgatgaagac gcgatccatc gtcaactgga cagcggtgca atagtgctaa
     2581 tggggccggt cgctgtttca gtcactggcg agagctttaa cctgacctcg gaagagattg
     2641 ccactcaact ggccatcaaa ctgaaagctg agaagatgat tggtttttgc tcttcccagg
     2701 gcgtcactaa tgacgacggt gatattgtct cagaactttt ccctaacgaa gcgcaagcgc
     2761 gggtagaagc ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg
     2821 gcgcagtgaa agcctgccgc agcggcgtgc gtcgctgtca tttaatcagt tatcaggaag
     2881 atggcgcgct gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg
     2941 aaagcgccga gcagattcgt cgcgcaacaa tcaacgatat cggcggtatt ctggagttga
     3001 ttcgcccgct ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg
     3061 aaatcgacaa attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct
     3121 atccgttccc ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc
     3181 gcagttcatc acggggcgag gttctgcttg aacgcattgc cgctcaggcg aagcagagcg
     3241 gcttaagcaa attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat
     3301 ttaccccggt ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc
     3361 gtaaatccaa agtgttgatg gcggatttag ggtaagcgac gaaaatcgcc ggatgcgaca
     3421 tgcgtaacac tcgtgcgtcg catccggcaa ttacgttaat tccggggatc cgtcgacctg
     3481 cagttcgaag ttcctattct ctagaaagta taggaacttc gaagcagctc cagcctacac
     3541 aagcggctcc tgactactga cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc
     3601 ccccgggtga aactagcgta acgctgatgc tgacacatcc cgcctgcgat gtctgcattc
     3661 ggttgacctg ccagttggca ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccagccat
     3721 gccgccagag ctgttggtac tggtttctgc tggcaagact gttcattaat gttcgctgga
     3781 aacccgataa cgcagtgaca atcatcacca tcaacaccat cgccaacatt acctccggca
     3841 ggctaaagcc ttgttgattc ttcagggaag ctgacataac gccctctctt tcagcggaca
     3901 aaaatcgctc cagccgcgtg gcgaaaaaac aatgtttcca tcaatgactt cgcctgttcg
     3961 ccacaacgaa acgccttcat aaccggcaat caataaggct tcattatctg ccagtaaacg
     4021 caaacaaacc tgggcatcgg tttcagcgta ctgcgagcac tgaactgctg gctgcgtctg
     4081 ccaggagtgc atttttcccc acgccagcgc cgactgaacg atggcctggc ggcgcaatga
     4141 ctgactttcc atgctcacgc gagaggcaaa actgcgatcc tgctgactca ttccttgcaa
     4201 tagcaagcta cccaaaacca gcagcatcag gaccagagcc agtgacgaaa cacctttttc
     4261 gcggttcaca ggttgaatcc tgtcacgcta tagctggcat ccaccacggt ttgcggttca
     4321 gacttactgg cagcacgcat attaaccgtc aacaccggcg agaagccgct gacatcctga
     4381 cgtacgacct gaaaagtgtc gataatgatg gcatccggat tagtgacttt atcccagccc
     4441 ttaccttcac aggatgtcgc accgcgtagc gtttccagca catgctcctt cagacgaaat
     4501 ccaatctggt cggactcttt taccggttcg cgatcccaga taccgttact gttcgcatcc
     4561 cactgcacaa tgatacagtc accctgtccg acaatttcca gcccttcgcc ggtacagctg
     4621 ccatgacaat aacccgccct ctggagatgc ttcgcgacgg taaatacccg cagccagatt
     4681 tcatcttcca gcgccagctt acgggtgccc gttaaacttt cacgctgtaa cgcaggcaga
     4741 aagcgtgccg ctcccagcaa caatacgcta ctgatcgcca tagcaatcaa cacttccagc
     4801 agagaaaaac cttgctcttt tacaggcatc cttctgtttc tccttgctga caaagccgga
     4861 gtcttcccca cggcgaaacc accagccacc actcgcccgt tgagtttttg aagcgaatat
     4921 gcccggccca tgcggtattg cgcaggccaa agaaagcaag cgaaggtgtc aggtcgctca
     4981 tttcgacttc gggccagcgt ggcacaaaga ccaatggtga actgccatga caggtattcg
     5041 ccccagcaac ggaactcaca aggcaccata acgtcccctc tctgataaca ctgatactgt
     5101 ggtcgcggtt atgccagttg gcatcttcac gtaaatagag caaatagtcc cgcgcctggc
     5161 tggcggtttg ccatagccgt tgcgactgct gccagtattg ccagccatag agtccacttg
     5221 cgcttagcat gaccagaatc agcatcgcga ccagcgtttc aatcagcgta taaccacgtt
     5281 gtgttttcat gccggcagta tggagcgagg agaaaaaaag acgagggcca gtttctattt
     5341 cttcggcgca tcttccggac tatttacgcc gttgcaggac attgcaaaat ttcgggaagg
     5401 cgtctcgaag aatttaacgg agggtaaaaa aaaccgacgc acactggcgt cggctctggc
     5461 aggatgtttc gtaattagat agccaccggc gctttaatgc ccggatgcgg atcgtagcct
     5521 tcaatctcaa agtcttcgaa actgtagtcg aagatggatt cgggtttacg tttgataatc
     5581 aacttcggca gcggacgcgg ttcgcggctt aattgcagat gagtttgatc catatgattg
     5641 ctgtacaggt gcgtatcgcc accggtccag acaaaatcac ccacttccag atcgcactgc
     5701 tgcgccatca tatgcaccag taacgcgtag ctggcaatgt tgaacggcag gccgaggaag
     5761 acgtcacagg agcgctgata aagctggcaa gagagtttgc cgtctgccac atagaactgg
     5821 aagaatgcat ggcacggtgc cagcgccatt ttatccagtt cgcctacgtt ccacgctgaa
     5881 acaataatgc ggcgcgaatc cgggtcgttt ttcagctggt tcagtaccgt agtgatctgg
     5941 tcaatatgac gaccatctgg cgttggccag gcgcgccact gtttaccata cactggcccg
     6001 aggtcgccgt tttcatcggc ccattcgtcc cagatggtga cattgttttc gtgtagataa
     6061 gcaatgttag tgtcgccctg caggaaccac agcagttcgt ggatgatgga acgcaggtgg
     6121 caacgtttag ttgtcaccag cgggaatcca tcctgcaggt taaaacgcat ctgatgacca
     6181 aaaatggaaa gcgttccggt tccggtacgg tcgtttttct gtgtgccttc gtcgagcact
     6241 ttttgcatca gttctaaata ctgtttcat
//
