LOCUS       pHS0537_CRISPR-associat 8010 bp ds-DNA     circular     04-JUL-2021
DEFINITION  .
SOURCE      Cloning vector pBR322                                              
  ORGANISM  Cloning vector pBR322 other sequences; artificial sequences;       
            vectors.
FEATURES             Location/Qualifiers
     rep_origin      39..721
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     -35_signal      115..120
                     /label="RNAII promoter (counter-clockwise); TTGAGA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      115..703
                     /label="pMB1 origin of replication (counter-clockwise)(RNAII -35 to RNA/DNA switch point)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     -10_signal      136..141
                     /label="RNAII promoter (counter-clockwise); CGTAAT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_RNA        151..703
                     /label="RNAII transcript (complementary strand)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_RNA        complement(154..261)
                     /label="RNAI transcript"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     -10_signal      complement(270..275)
                     /label="RNAI promoter (clockwise); GCTACA"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     -35_signal      complement(292..297)
                     /label="RNAI promoter (clockwise); TTGAAG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     gene            complement(1131..1322)
                     /label="rop gene"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             complement(1131..1322)
                     /label="rop CDS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     RBS             complement(1328..1332)
                     /label="rop RBS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(3030..3049)
                     /label="EcoRV left"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3050..6698
                     /label="CRISPR-associated IscB locus (Delaware Bay)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3399..3434
                     /label="DR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3435..3464
                     /label="Fn spacer (bottom)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3465..3500
                     /label="DR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(3608..3616)
                     /label="anti-repeat"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3832..5205
                     /label="IscB"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3832..5205
                     /label="Translation 3832-5205"
     misc_feature    complement(6699..6718)
                     /label="EcoRV right"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     -35_signal      6811..6822
                     /label="P1 promoter -35 sequence region; transcript starts36, 37 (complementary strand)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     -10_signal      6837..6842
                     /label="P1 promoter (counter-clockwise); TAAACT"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     -10_signal      complement(6848..6853)
                     /label="tet (P2) promoter (clockwise); TTTAAT; transcriptstart 45"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     -35_signal      complement(6871..6876)
                     /label="tet (P2) promoter (clockwise); TTGACA"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     -35_signal      7024..7029
                     /label="bla (P3) promoter (counter-clockwise); TTCAAA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     -10_signal      7045..7050
                     /label="bla (P3) promoter (counter-clockwise); GAGACA;transcript start 4188 (complementary strand)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     RBS             7082..7086
                     /label="bla RBS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     sig_peptide     7094..7162
                     /label="bla sig_peptide"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             7094..7954
                     /label="bla CDS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     gene            7094..7954
                     /label="bla gene"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             7094..7954
                     /label="Translation 7094-7954"
     CDS             7292..7951
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             7292..7951
                     /label="Translation 7292-7951"
ORIGIN
        1 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
       61 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
      121 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
      181 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
      241 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
      301 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
      361 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      421 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      481 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      541 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      601 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      661 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      721 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      781 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
      841 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
      901 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
      961 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     1021 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     1081 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     1141 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     1201 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     1261 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     1321 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     1381 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     1441 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     1501 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     1561 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     1621 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     1681 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     1741 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca
     1801 acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc
     1861 tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt
     1921 ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc
     1981 tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc
     2041 atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc
     2101 caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt
     2161 cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag
     2221 cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt
     2281 agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg
     2341 tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg
     2401 acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg
     2461 ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga
     2521 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
     2581 gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     2641 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     2701 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     2761 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     2821 cgccggtgat gccggccacg atgcgtccgg cgtagaggat ccacaggacg ggtgtggtcg
     2881 ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc
     2941 caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata
     3001 gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgatG ACAGTAGCCT
     3061 GCAAATGATG ACCTGGCCGG AACGCGAACG TAATCGCTGG CGGCGCTTCC TGAGTACCAA
     3121 GAATCATCCG GTGGACATTG TGGCTTTTGC GGAGCTCCGC TGGCTCTTGG ATGTTCCCGA
     3181 TCCGGTGATG CCAACGAAGG AAGAGATGGA GCGGAAGTAT AAGCGGCAGG CACGGTACGA
     3241 ACGGGAAAAG GAAAGGTGTT GCTTGTGATG GGCTCCAGAA GCCGATTGTT TACATCTGCA
     3301 CCCACTGGCG TTATATTCGG CCCGATTGAA GGTGGTGGCG CTATACTCAT TTTCGGGGGG
     3361 TTGTCAGGAA AAAGTTGGAA CTCAAACCAC TCCTCCCAGT TGTAGCCTCC ACTCATTTTC
     3421 GGGGTGCTGT CAGGTTTAAC AGTGGCCTTA TTAAATGACT TCTCGTTGTA GCCTCCACTC
     3481 ATTTTCGGGG TGCTGTCAGG GAGTGCCCGC GCTACCCTGC TTGGATGCTG GGCTGGAAGG
     3541 CAAAAGGCGT TCAAAAAAAT GGGCGGTGGT TGACAGTTTT CCGGAAATGA GTGCGGATTT
     3601 TTTCCGCAGC TACAACCAGA CTAAGTTCTT TGGGAACTAC GTTATTGTCG TCATCATACC
     3661 CACGGGTGCG TGCCAGCCTG TGGCTCTATG GTCGTGCATT AAACAGGGAC TTTGGAGGGT
     3721 GCCCAGTGTG TACGGCGTCA AAAGCGGCGA TAACATTGTC GAGGCATACT TTACGTGACG
     3781 GCAACGCTTC TTCGGAAGTT GCGCCCGATC GCTAGCTTTA AGGCTAATTC AATGTCCAGA
     3841 GTCTTGGTTG TTGACGCTGA TCGTTGTCCG CTGGCGCCTT GTACGCCAAG ACGGGCTCGC
     3901 CTGCTACTGA ACAGTGGCAA GGCAGCGGTG TTGCGTCGAT ACCCATTCAC TATCATCCTC
     3961 AAACAATCCT ACCCTACCGC AAGCCCACGA CCTGTGCGGC TGAAACTAGA TCCAGGCAGC
     4021 AAGACTACCG GCATTGCCGT AGTCACAGAA GCCACTGGCG AAGTGGTCTG GGCGGCAGAA
     4081 CTCCAGCACC GTGGTCAGCT GATCAAGAAT GCCCTGGAAT CCCGCCGATC GCTACGCCGT
     4141 GGAAGACGCA ACCGCAAGAC CCGCTACCGT CCTGCCCGTT GGCTGAATCG CAAGCGTACT
     4201 GGCCCACCTT TGCTATCCAG TGCTGACACG GTTTCTACGT TGGGGAAGTG GCTCGCTCCA
     4261 TCGCTCCAAC ATCGCATTGA AGTCATCATG ACCTGGGTGC ATCGCTTGCG GCGCTACCTG
     4321 CCCATCACGG CGATTTCACA AGAGATTGTG CGCTTTGATA TGCAGAAGAT GCAGAACCCG
     4381 GAAATCAGTG GCGTGGAGTA CCAGCAGGGC ACTCTATTCG GTTACGAGGT GCGGGAATAC
     4441 CTGTTGGACA AGTGGCGGCG ACAGTGCGGC TATTGCGGTG CCAAGGACAA GCGTTTGGAA
     4501 GTGGATCACA TTGTTCCACG TAGCCACGGC GGCAGTGATC GGGTCAGCAA CCTGACGCTA
     4561 TCTTGTGAAC CCTGCAACAA AAGAAAGAAC CAACGTCCTG CAGCCGTGTT CCTGGCAAAA
     4621 AAACCGGAAG TGTTGCAGAA ACTCCAACGT CAGGCCAAGG CTCCACTGAA GGATGCTGCA
     4681 GCCGTCAACT CAACCCGATA CGCTCTACTG GAACGTCTGA AAGCTACTGG CTTGCCTGTG
     4741 GAAGTTGCCA GCGGTGGCCG CACCAAGTTC AATCGTTCTG AGCGACAGAT TCCCAAGACA
     4801 CACTGGCTGG ATGCCGCTTG TGTCGGAGCC AGTACACCGG AAGTGCTGCA GTGGGAAGCG
     4861 GTCAGACCAC TAGCGATCAA GGCGATGGGG CACGGGAAAC GGCAGGTTGT CAACGTAGAT
     4921 GCTTACGGTT TCCCTAGAGG TAAGGCCAAA GGAATTCCGG TTCATCCGTT TCGCACCGGC
     4981 GACATTGTTC GGGCTGAAAT TCCCAAGGGC AAGTATGTCG GCACCTACGT CAGTCGTATT
     5041 GCCGAAACTA CAACATCGAA GCCTTTAGCT GGGTTCAAGT CCAAGACTGG TAAACGCATA
     5101 CAATGCCACA CAAAGCATAT GACCAAGCTC TTCAATTCCG ACGGCTATGG CTATGGCTTT
     5161 CTGAAGGCTC CAGAGCCACG CCAGACCGTT ATCAGCGAAT CCTGAACGAA AGCGCTACAA
     5221 AGAGCCCAAT CGCTCGTCCA GGCGCTTGAT TGCTGCCTGT GCCTCTGCCT TGTGCAGTTG
     5281 ACGTTCAGAA CGTGCAGCAT CGAGCTGCGC TTCCACACCT GCCGCCTTTG CAACCACTTG
     5341 CAGGTCTAGA CGTAGTTCGT CAATCTCCTG GCGCAACCGC TCTAGCTCTT CGCCGACTTC
     5401 CTTGAATCCG CTGATTGGCA TTTGTTTGAA CCCGTTATTA TGTTTTACAA AACCTAATAT
     5461 TGGTTTTGTG TTGTCGGTGG TAAGTGATGA GTTCCACTCG ATCTATTAGC GAACTCATAG
     5521 CGATGTTGCC CTTGATGGCT ACATTAGGAA TCTAACCACC AGTCAATTAC TGGCCTGACA
     5581 AGTGATAACA ACCCATAAGG GCAACAAAAA AGGCAACATT CATTACGAAA CAACTGTTGG
     5641 CTATATTGTT GGCTAGTATG CCTGTTTTTG ATTTTATCCC GCTTGAATCC TGACAATTCG
     5701 ATTCCTACGG TTAGCATCAG ACCTTCACGT GCGCGGCGTT AAGAAGACTG TTTGAATTCG
     5761 CAACACCCGC CACCTGGGCC GGTAGGGCAA AACAAGCGCA AGCGATCAAT TAGGTTCAGG
     5821 CGCGGTTAGC CGCGTTCTCA GCTGCCTCGA TTTCTTGCCG GTGCCTTGCC GCCCAATCTT
     5881 TCAAGCGACT ATCGGCCTTC CTTGCTTCCA GACGCCCAGC CCGTACAGCG GCGTCCACAT
     5941 CGCCACCACG CTGGATTCGC AACAACTGTT CGCCTGCATT GATCGGCAGC CGTCGCATTG
     6001 ACAATGCAGC TGCTAGTGGC GCTCCACGTC GCAACTCTTC CAATAGCTTG GATTGCTCAA
     6061 GCGGATCTGC AAAAAACTTC GCCAGTCTTA GCTTGGATTG CTGTTTCATA ATCGACCTTA
     6121 CTTTTGCCTA ACCTTTTACC TTACTTTTGC CTAACGCCAG CCTAACGCCA GCCTAACCGT
     6181 GATGACCCAT TGTGCCAACC TAACCTTACC TAACCTCCCC CCGTAGGGGG TAAGGTAAGG
     6241 TTGGGTAAGG CATCAATCCA GGTTAGGTAG CTTCAAAACT GATGTGGTCA GATCGCAAAA
     6301 AATATTCATC CCTTGAGCCC TCCTCACGAA CTAGAAAGAC ACCTGCCTCC ACCATCTTGT
     6361 CCTTGAGTTG TTTCTTGGTT TTGTCGTGGG GCGTGATACC CGCCACCTCA AGCCCTTTGT
     6421 ACCAAGCAGC TCTGGTCACA ATGCCATTTT CGCTTTGCGT TCTTAGCTCA AGGTATAGCG
     6481 CTCTTGCTTC CTGCTGCTTG CTTGTTAGTG TCTGAGCCTT AGTTGGTTCA GCGATTTCGC
     6541 ATTGCTCAAG AATGCAAGAC TGGACAGGGC TTCCATCGTC TTCTGCTAGT GGCCGCCCTA
     6601 GTTCGTCGAG AACGTCAACC GGCTCCAGTT TGAAGCCTAT CGGCGCAAAC GGCTCTGCAT
     6661 CCTTCGACTT GGTGCAGGTT AGGTTAATGA ATCCTCTAat cccgcaagag gcccggcagt
     6721 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
     6781 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
     6841 ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa
     6901 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     6961 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7021 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7081 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7141 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7201 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7261 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7321 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     7381 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7441 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7501 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7561 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7621 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7681 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7741 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     7801 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     7861 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     7921 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     7981 actttagatt gatttaaaac ttcattttta
//