LOCUS       pHS0535_CRISPR-associat 7178 bp ds-DNA     circular     04-JUL-2021
DEFINITION  .
SOURCE      Cloning vector pBR322                                              
  ORGANISM  Cloning vector pBR322 other sequences; artificial sequences;       
            vectors.
FEATURES             Location/Qualifiers
     gene            complement(57..917)
                     /label="bla gene"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(57..917)
                     /label="bla CDS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(57..917)
                     /label="Translation 917-57"
     CDS             complement(60..719)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(60..719)
                     /label="Translation 719-60"
     sig_peptide     complement(849..917)
                     /label="bla sig_peptide"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     RBS             complement(925..929)
                     /label="bla RBS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     -10_signal      complement(961..966)
                     /label="bla (P3) promoter (counter-clockwise); GAGACA;transcript start 4188 (complementary strand)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     -35_signal      complement(982..987)
                     /label="bla (P3) promoter (counter-clockwise); TTCAAA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     source          join(1126..7178,1..1125)
                     /label="source"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     -35_signal      1135..1140
                     /label="tet (P2) promoter (clockwise); TTGACA"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     -10_signal      1158..1163
                     /label="tet (P2) promoter (clockwise); TTTAAT; transcriptstart 45"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     -10_signal      complement(1169..1174)
                     /label="P1 promoter (counter-clockwise); TAAACT"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     -35_signal      complement(1189..1200)
                     /label="P1 promoter -35 sequence region; transcript starts36, 37 (complementary strand)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     gene            1211..5218
                     /label="tet gene"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1211..5218
                     /label="tet CDS"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             1214..5215
                     /label="TetR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1293..1312
                     /label="EcoRV right"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1313..4129)
                     /label="00644 locus 1"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1531..1571
                     /label="End?"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(1873..3246)
                     /label="IscB"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(1873..3246)
                     /label="Translation 3246-1873"
     misc_feature    3251..3280
                     /label="hyRNA probe 6"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3316..3345
                     /label="hyRNA probe 5"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3359..3388
                     /label="hyRNA probe 4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3408..3437
                     /label="hyRNA probe 3"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3462..3470
                     /label="tracr anneal?"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3471..3500
                     /label="hyRNA probe 2"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3520..3549
                     /label="hyRNA probe 1"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(3577..3612)
                     /label="DR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3583..3612
                     /label="DR probe 1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3596..3625
                     /label="DR probe 2"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(3613..3642)
                     /label="Fn spacer (bottom)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(3643..3678)
                     /label="DR"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(3709..3744)
                     /label="DR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(3775..3810)
                     /label="DR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    4130..4149
                     /label="EcoRV left"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     RBS             5847..5851
                     /label="rop RBS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     gene            5857..6048
                     /label="rop gene"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             5857..6048
                     /label="rop CDS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(6458..7140)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_RNA        complement(6476..7028)
                     /label="RNAII transcript (complementary strand)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     rep_origin      complement(6476..7064)
                     /label="pMB1 origin of replication (counter-clockwise)(RNAII -35 to RNA/DNA switch point)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     -35_signal      6882..6887
                     /label="RNAI promoter (clockwise); TTGAAG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     -10_signal      6904..6909
                     /label="RNAI promoter (clockwise); GCTACA"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_RNA        6918..7025
                     /label="RNAI transcript"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     -10_signal      complement(7038..7043)
                     /label="RNAII promoter (counter-clockwise); CGTAAT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     -35_signal      complement(7059..7064)
                     /label="RNAII promoter (counter-clockwise); TTGAGA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
       61 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
      121 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
      181 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
      241 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
      301 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      361 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      421 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      481 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
      541 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      601 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      661 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
      721 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      781 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      841 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      901 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      961 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1021 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     1081 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattctc atgtttgaca
     1141 gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct aacgcagtca
     1201 ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga
     1261 tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atTTTTATTT
     1321 CTGATTAGAG AAATTTTCTT CTGTTTTGAA TGGCTGCTGA TCAACCAGAA AAGCCTAAAG
     1381 AGAATCAACT GGGAGGTAGT TAAAAGGCAA GCGCGATGTT GGTTATCGTT GATTTCCAGA
     1441 GTCAATTGTT CCTAATGAAT GTTGCCCTTT TTGTTGCCCT TAAGAAGTGT TATCACCGGA
     1501 CAGGCCAGTA ATTATCTAGT GATTAGATTC CTAACGTAGC CATCAAGGGC AACATCGCTA
     1561 TGAGTTCGAT AACGTTCCGA GTGGAACTCA TCACTTACCA CCGACAACAC AAAACCAATA
     1621 TTAGGTTTTG CAAAACATAA TAACGGGTTC AAACAAATGC CAATCAGCGA ATTCAAGGAA
     1681 GTCGGCGAAG AGCTAGAGCG GTTGCGCCAG GAGATTGGCG AACTACGTCT AGACCTGCAA
     1741 GTAGTTGCAA AGGCGGCAGG TGTAGAAGCG CAGCTGGACG CTGCACGTTC AGAACGCCAA
     1801 CTGCATAAGG CTGAGACGCA AACAGCGATC AAGCGTCTGG ATGAGCGATT GGGCTCTTTG
     1861 TAGCGCTTTC GTTCAGGATT CGCTGATAAC GGTCTGGCGT GGCTCTGGAG CCTTCAGAAA
     1921 GCCATAGCCA TAGCCGTCGG AATTGAAGAG CTTGGTCATA TGCTTTGTGT GGCATTGTAT
     1981 GCGTTTACCA GTCTTGGACT TGAACCCAGC TAAAGGCTTC GATGTTGTAG TTTCGGCAAT
     2041 ACGACTGACG TAGGTGCCGA CATACTTGCC CTTGGGAATT TCAGCCCGAA CAATGTCGCC
     2101 GGTGCGAAAC GGATGAACCG GAATTCCTTT GGCCTTACCT CTAGGGAAAC CGTAAGCATC
     2161 TACGTTGACG ACCTGCCGTT TCCCGTGTCC CATCGCCTTG ATCGCCAAAG GCTTGACTGC
     2221 TTTCCACTGC AGCACTTCCG GTGTACTGGC TCCGACACAA GCGGCATCCA GCCAGTGTGT
     2281 CTTGGGAATC TGTCGCTCAG AACGATTGAA CTTGGTGCGG CCACCGCTGG CAATTTCCAC
     2341 AGGCAATCCA GTGGCCTTCA GACGTTCCAG TAGAGCGTAT CGGGTTGAGT TGACGGCTGC
     2401 AGCATCCTTG AGCGGAGCCT TGGCCTGACG TTGGAGTTTC TGCAACACTT CCGGTTTTTT
     2461 TGCCAGGAAT ACTGCCGCAG GACGTTGGTT CTTTCTTTTG TTGCAGGGTT CACAAGATAG
     2521 CGTCAGGTTG CTGACCCGAT CACTGCCGCC GTGGCTACGT GGAACAATGT GATCCACTTC
     2581 CAAACGCTTG TCCTTGGCAC CGCAATAGCC GCACTGTCGC TGCCACTTGT CCAACAGGTA
     2641 TTCCCGCACC TCGTAACCGA ACAGAGTGCC CTGCTGGTAC TCCACGCCAC TGATTTCCGG
     2701 GTTCTGCATC TTCTGCATAT CAAAGCGCAC AATCTCTTGT GAAATCGCCG TGATGGGCAG
     2761 GTAGCGCCGC AAGCGATGCA CCCAGGTCAT GATGACTTCA ATGCGATGTT GGAGCGATGG
     2821 AGCGAGCCAC TTCCCCAACG TAGAAACCGT GTCAGCACTG GATAGCACAG GTGGGCCGGT
     2881 ACGCTTGCGA TTCAGCCAAC GGGCAGGACG GTAGCGGGTC TTGCGGTTGC GTCTTCCACG
     2941 GCGTAGCGAT CGGCGGGATT CCAGGGCATT CTTGATCAGC TGACCACGGT GCTGGAGTTC
     3001 TGCCGCCCAG ACCACTTCGC CAGTGGCTTC TGTGACTACG GCAATGCCGG TAGTCTTGCT
     3061 GCCTGGGTCT AGTTTCAGCC GCACAGGTCG TGGGTTTGCA GTAGGGTAGG ATTGTTTGAG
     3121 GATGATGGTG AATGGGTATC GACGCAACAC CGCCGCCTTG CCACTGTTCA GTAGCAAGCG
     3181 GGCCCGTCTT GGCGTACAAG GCGCCAGCGG ACAACGATTC GCATCAACAA CCAAGACTCT
     3241 GGACATTGAA TTAGCCTTAA AGCTAGCGAT CGGGCGCAAC TTCCGAAGAA GCGTTGCCGT
     3301 CACGTAAAGT ATGCCTCGAC AATGTTATCG CCGCTTTTGA CGCTGTACAC ACTGGGCACC
     3361 CTCCAAAGTC CCTGTTTAAT GCACGACCAT AGAGCCACAG GCTGGCACGC ACCCGTGGGT
     3421 ATGATGACGA CAATAACGTA GTTCCCAAAG AACTTAGTCT GGTTGTAGCT GCGGAAAAAA
     3481 TCCGCACTCA TTTCCGGAAA ACTGTCAACC ACCGCCCATT TTTTTGAATG CCTTTTGCCT
     3541 TCCAGCCCAG CATCCAAGCA GGGTAGCGCG GGCACTCCTG ACAGCACCCC GAAAATGAGT
     3601 GGAGGCTACA ACGAGAAGTC ATTTAATAAG GCCACTGTTA AACCTGACAG CACCCCGAAA
     3661 ATGAGTGGAG GCTACAACGA TTCCCAGACT CCAGATGAAT CCACCCACCC TGACAGCACC
     3721 CCGAAAATGA GTGGAGGCTA CAACATCGAC TTTCGCCTGA GCTAAACGCA TCAGCCTGAC
     3781 AGCACCCCGA AAATGAGTGG AGGCTACAAC TACCCCGTTG GGTTCAGCCC AAACTGACCA
     3841 AACTGACAGC ACCCCGCATC TTCGATCGGG CCGACGAATA TAACGCCAGT GGGTGCAGAT
     3901 GTAAACAGGT AGGCTGCAGA TGGCTTGTGG TGCAGTGTTT TGGCCAGGGA TGTGGTTGCG
     3961 TTCAGTGCTT TTTATACGCC TACTGACGGG CCTTCTGAAG CTTCTGGTGG CGTCTGGTAT
     4021 TCAGCAGCTG CATAGCCCGC TGACGGCGGC GTAGAGCGCT TGGAGGCGCA AGCATCTCCT
     4081 GAATGATCGA CACTGGTGCA TCTGGCGGTA GTGAATGGAT CGCATAGACa tcgtccattc
     4141 cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct
     4201 atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc
     4261 ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat
     4321 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     4381 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     4441 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     4501 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     4561 cttcctaatg caggagtcgc ataagggaga gcgtcgaccg atgcccttga gagccttcaa
     4621 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
     4681 cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga
     4741 ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt
     4801 gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca
     4861 ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac
     4921 gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc
     4981 cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg
     5041 atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat
     5101 ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata
     5161 ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat
     5221 ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc
     5281 ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc
     5341 atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc
     5401 atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag
     5461 cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg
     5521 acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg
     5581 aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc
     5641 tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc
     5701 tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca
     5761 tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc
     5821 cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc
     5881 cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag
     5941 ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt
     6001 taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     6061 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     6121 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     6181 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     6241 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     6301 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6361 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6421 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6481 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6541 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6601 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6661 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6721 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6781 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6841 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6901 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6961 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7021 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7081 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7141 gattatcaaa aaggatcttc acctagatcc ttttaaat
//