LOCUS       Exported               10505 bp DNA     circular SYN 18-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
COMMENT     resequencing for what is in plasmid library is perfect!
FEATURES             Location/Qualifiers
     source          1..10505
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             61..341
                     /label=3'LTR
                     /label=3'LTR (dU3)
     primer_bind     complement(367..384)
                     /label=SP6
     old_sequence    1077..3084
                     /label=pBR322
     CDS             1278..2138
                     /codon_start=1
                     /label=bla
                     /label=bla (AmpR)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1476..2135
                     /label=AmpR
     rep_origin      2233..2915
                     /label=ColE1 origin
     promoter        3276..3472
                     /label=SV40 early promoter
     polyA_signal    complement(5185..5376)
                     /label=SV40 late polyA
     LTR             5549..6692
                     /label=5'-LTR
     misc_structure  6700..7556
                     /label=RRE
     repeat_region   7565..7744
                     /label=cPPT
     primer_bind     7720..7743
                     /label=cPPT_5'seq
     promoter        7777..8268
                     /label=SFFV
     primer_bind     8193..8216
                     /label=1166_seq
     misc_feature    8296..8301
                     /label=Mlu1
     misc_feature    8311..8379
                     /label=CD3_epsilon signal peptide
     misc_feature    8380..8406
                     /label=HA tag
     misc_feature    8437..8979
                     /label=SNAPf
     misc_feature    8904..8921
                     /label=SNAP_for
     misc_feature    8980..9006
                     /label=Linker
     primer_bind     8980..9006
                     /label=FKBP-Link_Rev
     primer_bind     8982..9006
                     /label=Link-CD86_For
     CDS             9007..9114
                     /codon_start=1
                     /label=CD86TM
                     /translation="PPDHIPWITAVLPTVIICVMVFCLILWKWKKKKRPR"
     misc_feature    9022..9114
                     /label=TM_region
     primer_bind     9087..9114
                     /label=CD86-ZetaCyt_For
     primer_bind     9087..9114
                     /label=CD86-ZetaCyt_Rev
     primer_bind     9096..9150
                     /label=5'_pHR-SP-HA-SNAP-CD86tm-NoZeta-mGFP
     misc_feature    9127..9135
                     /label=Ser-Gly-Gly linker
     misc_feature    9136..9852
                     /label=EGFP
     primer_bind     complement(9838..9882)
                     /label=3'_pHR-SNAP-CD86tm-zeta-mGFP_Rv
     misc_feature    9856..9863
                     /label=Not1
     primer_bind     9905..9927
                     /label=1167_seq
     enhancer        9914..10505
                     /label=WPRE
ORIGIN
        1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
       61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
      121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
      181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
      361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
      421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
      481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
      541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
      601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
      901 tctcagtaca atctagctct gatgccgcat agttaagcca gccccgacac ccgccaacac
      961 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     1021 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     1081 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1141 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1201 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1261 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1321 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1381 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1441 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1501 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1561 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1621 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1681 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1741 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1801 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1861 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1921 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     1981 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2041 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2101 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2161 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2221 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2281 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2341 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2401 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     2461 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2521 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2581 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2641 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2701 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2761 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2821 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2881 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2941 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3001 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3061 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3121 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3181 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3301 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3361 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3421 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     3481 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     3541 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     3601 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     3661 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     3721 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     3781 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     3841 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     3901 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     3961 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     4021 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
     4081 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     4141 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
     4201 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     4261 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     4321 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     4381 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
     4441 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
     4501 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
     4561 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
     4621 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
     4681 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
     4741 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
     4801 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
     4861 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
     4921 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
     4981 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
     5041 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
     5101 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     5161 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     5221 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     5281 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5341 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     5401 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt
     5461 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     5521 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     5581 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     5641 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     5701 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     5761 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     5821 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     5881 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     5941 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     6001 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6061 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6121 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6181 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     6241 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     6301 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     6361 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     6421 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     6481 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     6541 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     6601 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     6661 caaaacaaaa gtaagaccac cgcacagcaa gcggccggtg atcttcagac ctggacgata
     6721 tatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     6781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     6841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     6901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     6961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     7021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     7081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     7141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     7201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     7261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     7321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     7381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     7441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     7501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     7561 cggtcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     7621 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     7681 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     7741 ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa gattttattt
     7801 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagctgca
     7861 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
     7921 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
     7981 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
     8041 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
     8101 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
     8161 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
     8221 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag
     8281 ctctcgagaa ttctcacgcg tgccgccacc atgcagtcgg gcactcactg gagagttctg
     8341 ggcctctgcc tcttatcagt tggcgtttgg gggcaagatt acccatacga cgtcccagac
     8401 tacgctctgc ccgagaccgg cggcggcggc ggcggcgaca aagactgcga aatgaagcgc
     8461 accaccctgg atagccctct gggcaagctg gaactgtctg ggtgcgaaca gggcctgcac
     8521 cgtatcatct tcctgggcaa aggaacatct gccgccgacg ccgtggaagt gcctgcccca
     8581 gccgccgtgc tgggcggacc agagccactg atgcaggcca ccgcctggct caacgcctac
     8641 tttcaccagc ctgaggccat cgaggagttc cctgtgccag ccctgcacca cccagtgttc
     8701 cagcaggaga gctttacccg ccaggtgctg tggaaactgc tgaaagtggt gaagttcgga
     8761 gaggtcatca gctacagcca cctggccgcc ctggccggca atcccgccgc caccgccgcc
     8821 gtgaaaaccg ccctgagcgg aaatcccgtg cccattctga tcccctgcca ccgggtggtg
     8881 cagggcgacc tggacgtggg gggctacgag ggcgggctcg ccgtgaaaga gtggctgctg
     8941 gcccacgagg gccacagact gggcaagcct gggctgggtg gcggaagcgg aggtagtggt
     9001 ggaagccccc cagaccacat tccttggatt acagctgtac ttccaacagt tattatatgt
     9061 gtgatggttt tctgtctaat tctatggaaa tggaagaaga agaagcggcc tcggagcgca
     9121 gacgccagcg gcggcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     9181 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     9241 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     9301 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     9361 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     9421 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     9481 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     9541 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     9601 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     9661 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     9721 ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag
     9781 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     9841 gagctgtaca agtaagcggc cgcgactcta gagtcgacct gcaggcatgc aagcttgata
     9901 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     9961 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
    10021 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
    10081 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
    10141 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
    10201 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
    10261 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
    10321 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
    10381 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
    10441 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
    10501 cgcat
//
