LOCUS       Exported               10168 bp DNA     circular SYN 24-AUG-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Meghan
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10168
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     43..66
                     /label=cPPT_5'seq
     promoter        100..591
                     /label=SFFV Pr
                     /label=SFFV\Pr
                     /note="/vntifkey=29"
     primer_bind     497..520
                     /label=1166_seq
     primer_bind     595..642
                     /label=Fragment 2.FOR
     misc_feature    619..624
                     /label=mluI
     misc_feature    625..963
                     /label=Fragment 1 gibson
     misc_feature    625..684
                     /label=beta 2 microglobin signal peptide
     misc_feature    625..684
                     /label=beta 2 microglobin signal peptide
                     /label=beta 2 microglobin signal peptide(1)
     misc_feature    625
                     /label=SpeI
     misc_feature    626..684
                     /label=Signal peptide for b2m
     misc_feature    685..963
                     /label=1xFRB
     primer_bind     complement(943..988)
                     /label=Fragment 2.REV
     primer_bind     complement(944..963)
                     /label=292
     primer_bind     964..990
                     /label=FKBP-Link_Rev
     misc_feature    964..990
                     /label=Linker
     primer_bind     964..983
                     /label=Fragment 1.FOR
     misc_feature    964..965
                     /label=(GS)3G4S2
     primer_bind     966..990
                     /label=Link-CD86_For
     CDS             991..1098
                     /codon_start=1
                     /label=CD86TM
                     /translation="PPDHIPWITAVLPTVIICVMVFCLILWKWKKKKRPR"
     misc_feature    1006..1098
                     /label=TM_region
     primer_bind     1071..1098
                     /label=CD86-ZetaCyt_Rev
     primer_bind     1071..1098
                     /label=CD86-ZetaCyt_For
     primer_bind     1080..1134
                     /label=5'_pHR-SP-HA-SNAP-CD86tm-NoZeta-mGFP
     primer_bind     complement(1091..1102)
                     /label=Fragment 2.FOR
     misc_feature    1111..1119
                     /label=Ser-Gly-Gly linker
     misc_feature    1120..1836
                     /label=EGFP
     primer_bind     complement(1822..1866)
                     /label=3'_pHR-SNAP-CD86tm-zeta-mGFP_Rv
     misc_feature    1840..1847
                     /label=Not1
     primer_bind     complement(1847..1870)
                     /label=Fragment 1.REV
     primer_bind     1847..1860
                     /label=Fragment 1.REV
     primer_bind     1889..1911
                     /label=1167_seq
     misc_feature    1898..2489
                     /label=WPRE
                     /note="/vntifkey=21"
     LTR             2523..2831
                     /label=3'-LTR (dU3)
                     /label=3'-LTR\(dU3)
                     /note="/vntifkey=19"
     misc_feature    3101..5573
                     /label=pBR322
                     /label=nonstandard type:  insertion_seq
                     /note="/vntifkey=14"
     CDS             3767..4627
                     /label=bla (AmpR)
                     /label=bla\(AmpR)
                     /note="/vntifkey=4"
     primer_bind     complement(5558..5572)
                     /label=1166_seq
     promoter        5681..5936
                     /label=SV40 Pr/Enh
                     /label=SV40\Pr/Enh
                     /note="/vntifkey=29"
     CDS             6161..6618
                     /label=gpt
                     /note="/vntifkey=4"
     promoter        7020..7869
                     /label=SV40 polyA+Intron
                     /label=SV40\polyA+Intron
                     /note="/vntifkey=29"
     LTR             8038..8722
                     /label=5'-LTR
                     /note="/vntifkey=19"
     misc_feature    8723..8860
                     /label=Psi
                     /note="/vntifkey=21"
     CDS             8861..10045
                     /label=HIV genes
                     /label=HIV\genes
                     /note="/vntifkey=4"
     misc_feature    join(10059..10168,1..68)
                     /label=cPPT
                     /note="/vntifkey=21"
ORIGIN
        1 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
       61 agtttggatc gataagcttg atatcgaatt cctgcagccc cgataaaata aaagatttta
      121 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct
      181 gcagtaacgc cattttgcaa ggcatggaaa aataccaaac caagaataga gaagttcaga
      241 tcaagggcgg gtacatgaaa atagctaacg ttgggccaaa caggatatct gcggtgagca
      301 gtttcggccc cggcccgggg ccaagaacag atggtcaccg cagtttcggc cccggcccga
      361 ggccaagaac agatggtccc cagatatggc ccaaccctca gcagtttctt aagacccatc
      421 agatgtttcc aggctccccc aaggacctga aatgaccctg cgccttattt gaattaacca
      481 atcagcctgc ttctcgcttc tgttcgcgcg cttctgcttc ccgagctcta taaaagagct
      541 cacaacccct cactcggcgc gccagtcctc cgacagactg agtcgcccgg gggggatctg
      601 gagctctcga gaattctcac gcgtatgtct cgctccgtgg ccttagctgt gctcgcgcta
      661 ctctctcttt ctggcctgga ggcaatcctc tggcatgaga tgtggcatga aggcctggaa
      721 gaggcatctc gtttgtactt tggggaaagg aacgtgaaag gcatgtttga ggtgctggag
      781 cccttgcatg ctatgatgga acggggcccc cagactctga aggaaacatc ctttaatcag
      841 gcctatggtc gagatttaat ggaggcccaa gagtggtgca ggaagtacat gaaatcaggg
      901 aatgtcaagg acctcctcca agcctgggac ctctattatc atgtgttccg acgaatctca
      961 aagggcggaa gcggaggtag tggtggaagc cccccagacc acattccttg gattacagct
     1021 gtacttccaa cagttattat atgtgtgatg gttttctgtc taattctatg gaaatggaag
     1081 aagaagaagc ggcctcggag cgcagacgcc agcggcggca tggtgagcaa gggcgaggag
     1141 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1201 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1261 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1321 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1381 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1441 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1501 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1561 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1621 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1681 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
     1741 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1801 gccgggatca ctctcggcat ggacgagctg tacaagtaag cggccgcgac tctagagtcg
     1861 acctgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     1921 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     1981 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     2041 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     2101 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     2161 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     2221 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     2281 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     2341 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     2401 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     2461 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaggg aattaattcg
     2521 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     2581 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     2641 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     2701 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     2761 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     2821 atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt
     2881 gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc
     2941 ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg
     3001 tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg
     3061 aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca
     3121 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     3181 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     3241 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     3301 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     3361 cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctagctctg atgccgcata
     3421 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     3481 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     3541 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     3601 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     3661 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3721 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3781 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3841 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3901 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3961 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4021 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4081 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     4141 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     4201 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     4261 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4321 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4381 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4441 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4501 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4561 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4621 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4681 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4741 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4801 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4861 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4921 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     4981 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5041 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5101 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5161 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5221 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5281 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5341 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5401 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5461 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     5521 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     5581 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     5641 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5701 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5761 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5821 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5881 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5941 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
     6001 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
     6061 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
     6121 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
     6181 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
     6241 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
     6301 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
     6361 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
     6421 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
     6481 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
     6541 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
     6601 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
     6661 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
     6721 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
     6781 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
     6841 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
     6901 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
     6961 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
     7021 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     7081 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     7141 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     7201 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     7261 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
     7321 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     7381 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     7441 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
     7501 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
     7561 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
     7621 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     7681 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     7741 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7801 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7861 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
     7921 cctattacca ctgccaatta cctagtggtt tcatttactc taaacctgtg attcctctga
     7981 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     8041 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     8101 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     8161 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     8221 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     8281 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     8341 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     8401 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     8461 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     8521 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8581 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8641 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     8701 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     8761 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8821 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8881 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8941 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     9001 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     9061 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     9121 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     9181 cggccggtga tcttcagacc tggacgatat atatgaggga caattggaga agtgaattat
     9241 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     9301 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     9361 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     9421 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     9481 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     9541 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     9601 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     9661 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     9721 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     9781 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     9841 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     9901 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     9961 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
    10021 gatccattcg attagtgaac ggatctcgac ggtatcgcca aatggcagta ttcatccaca
    10081 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
    10141 tagcaacaga catacaaact aaagaatt
//
