LOCUS       Exported                8244 bp ds-DNA     circular SYN 23-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...1367881019035.10
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 4, 2021 from SnapGene 4.0.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    887..917
                     /label=31bp spacer
                     /note="/ApEinfo_fwdcolor=#43ffb8
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ff77e0"
     repeat_region   887..917
                     /label=5'TR(1)
                     /label=nonstandard type: repeat_unit
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=5'TR
                     /ApEinfo_revcolor=green"
     repeat_region   join(921..936,918..919,874..886)
                     /label=5'TR
                     /label=nonstandard type: repeat_unit
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
     enhancer        1112..1343
                     /label=core insul
                     /note="/ApEinfo_fwdcolor=#d7cccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
     misc_feature    1390..1408
                     /label=TetO
     misc_feature    1426..1444
                     /label=TetO
     misc_feature    1462..1480
                     /label=TetO
     misc_feature    1498..1516
                     /label=TetO
     misc_feature    1534..1552
                     /label=TetO
     misc_feature    1570..1588
                     /label=TetO
     misc_feature    1611..1628
                     /label=42bs_42bs
     misc_feature    1629..1646
                     /label=42bs_42bs
     misc_feature    1647..1664
                     /label=42bs_42bs
     misc_feature    1686..1707
                     /label=37bs_37bs
     misc_feature    1722..1743
                     /label=37bs_37bs
     misc_feature    1758..1779
                     /label=37bs_37bs
     misc_feature    1794..1815
                     /label=37bs_37bs
     misc_feature    1830..1851
                     /label=37bs_37bs
     misc_feature    1866..1887
                     /label=37bs_37bs
     misc_feature    1899..2043
                     /label=miniCMV
                     /note="/ApEinfo_fwdcolor=#fff95e
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
     misc_feature    2060..2065
                     /label=Kozak-H2B F
                     /note="Geneious name: Kozak-H2B F
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     ApEinfo_fwdcolor=pink"
     CDS             2066..2068
                     /codon_start=1
                     /label=Start
                     /translation="M"
     CDS             2072..2092
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2096..2416
                     /codon_start=1
                     /label=FKBP12 F36V
                     /label=FKBP-Caspase9
                     /note="/ApEinfo_fwdcolor=#d59687
                     /ApEinfo_revcolor=#d59687"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             2423..2683
                     /codon_start=1
                     /label=37ZFm4new
                     /translation="EAPFQCRICMANFSRNFILQRHTRTHTGEKPFQCRICMANFSDRA
                     NLRRHLRTHTGEKPFQCRICMANFSRHDQLTRHLKTHTGEKG"
     misc_feature    2426..2428
                     /label=R2A
     misc_feature    2432..2440
                     /label=beta sheet
     misc_feature    2438..2440
                     /label=Cys1
     misc_feature    2447..2449
                     /label=Cys2
     misc_feature    2453..2461
                     /label=beta sheet
     misc_feature    2453..2455
                     /label=R11A
     misc_feature    2465..2485
                     /label=Finger 1
     misc_feature    2468..2506
                     /label=alpha helix
     misc_feature    2516..2524
                     /label=beta sheet
     misc_feature    2522..2524
                     /label=Cys1
     misc_feature    2531..2533
                     /label=Cys2
     misc_feature    2537..2545
                     /label=beta sheet
     misc_feature    2537..2539
                     /label=R39A
     misc_feature    2549..2569
                     /label=Finger 2
     misc_feature    2552..2590
                     /label=alpha helix
     misc_feature    2600..2608
                     /label=beta sheet
     misc_feature    2606..2608
                     /label=Cys1
     misc_feature    2615..2617
                     /label=Cys2
     misc_feature    2621..2629
                     /label=beta sheet
     misc_feature    2621..2623
                     /label=R67A
     misc_feature    2633..2653
                     /label=Finger 3
     misc_feature    2636..2674
                     /label=alpha helix
     CDS             2699..2932
                     /codon_start=1
                     /label=VP16
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             2939..2959
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2969..2992
                     /codon_start=1
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2999..3472
                     /codon_start=1
                     /label=DHFR
                     /translation="ISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     misc_feature    3489..4074
                     /label=IRES
     CDS             4075..4791
                     /codon_start=1
                     /label=mCitrine
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4798..4923
                     /codon_start=1
                     /label=PEST
                     /translation="KLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    4958..5165
                     /label=BGHpA
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        complement(5193..5423)
                     /label=core insul(1)
                     /note="/ApEinfo_fwdcolor=#d8cac8
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=core insul
                     /ApEinfo_revcolor=#d8c8c8"
     repeat_region   5698..5732
                     /label=3'TR
                     /label=nonstandard type: repeat_unit
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
     CDS             complement(7387..8244)
                     /label=Amp
                     /note="/ApEinfo_fwdcolor=#7ab3ee
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#c3c2c4"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtggcctcca cggccacaag
     1381 cttcatatgt ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     1441 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     1501 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     1561 acagtttact ccctatcagt gatagagaac gtatatccag tttactcccc gacgctgctg
     1621 acgctgctga cgctgctgac gctgctgacg ctgctgacgc tgctcgtcgc caattgtcgc
     1681 cggcgtgagg acgtgttgag gacgtgttct agagcctatt ttgaggacgt gttgaggacg
     1741 tgtatcgata tatttagtga ggacgtgttg aggacgtgtt cacgtactat ctatgaggac
     1801 gtgttgagga cgtgttatga cctaaggagt gaggacgtgt tgaggacgtg tacgtatata
     1861 cgcgttgagg acgtgttgag gacgtgtcct agaccggttg atagagaacg tataagcttt
     1921 aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt agtgaaccgt cagatcgcct
     1981 ggagcaattc cacaacactt ttgtcttata ccaactttcc gtaccacttc ctaccctcgt
     2041 aaatcgacga agcttctagg ccaccatggg acccaagaaa aagcggaagg tgtccggagt
     2101 gcaggtggag actatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg
     2161 cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa gttgattcct cccgggacag
     2221 aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg
     2281 ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta
     2341 tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga
     2401 gcttctaaaa ctggaaggat ccgaggcccc attccagtgc agaatctgca tggctaactt
     2461 ctcccggaac ttcatcctgc agagacacac cagaacacac acaggcgaga agcccttcca
     2521 gtgccggatc tgtatggcca acttttccga cagagccaac ctgcggagac acctgagaac
     2581 ccataccggc gaaaagcctt ttcagtgtcg catctgcatg gccaatttca gcaggcacga
     2641 ccagctgaca agacatctga aaacccacac tggcgagaaa ggcggcggat cctctggcgc
     2701 tcctcctacc gatgtgtctc tgggagatga gctgcacctg gacggcgagg atgtggctat
     2761 ggctcatgcc gacgctctgg acgacttcga cttggatatg ctcggcgacg gcgactctcc
     2821 tggacctggc tttacccctc atgactctgc cccttacggc gccctggata tggccgattt
     2881 cgagttcgag cagatgttca ccgatgctct gggcatcgat gagtacggcg gaggctcccc
     2941 caagaaaaag cggaaggtgg gctcgagcga ctacaaggac gacgacgaca agtcgagcat
     3001 ctccctgatc gctgccctgg ctgtggatta cgtgatcgga atggaaaacg ccatgccatg
     3061 gaacctgcct gctgacctgg cctggttcaa gcggaacacc ctgaacaagc ccgtgatcat
     3121 gggcagacac acctgggagt ctatcggcag acctctgcct ggccggaaga acatcatcct
     3181 gtcctctcag ccttccaccg acgacagagt gacctgggtc aagtctgtgg acgaggctat
     3241 cgctgcttgt ggcgacgtgc cagagatcat ggttatcggc ggaggcagag tgatcgagca
     3301 gttcctgcct aaggctcaga agctgtatct gacccacatc gacgccgaag tggaaggcga
     3361 tacccacttt cctgactacg agcccgacga ctgggagtcc gtgttctctg agttccacga
     3421 cgccgatgct cagaactccc actcctactg cttcgagatc ctggagcgga gataggtcga
     3481 ccagatcccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     3541 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     3601 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     3661 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     3721 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     3781 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     3841 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     3901 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     3961 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     4021 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggtg
     4081 agcaagggcg aggagctgtt caccggcgtg gtgcccattc tggtggaact ggatggggat
     4141 gtgaacggcc acaagttctc cgtgtctggc gaaggcgaag gggatgctac ctacggcaag
     4201 ctgaccctga agttcatctg caccaccgga aagctgcccg tgccttggcc tacactggtc
     4261 accacctttg gctacggcct gatgtgcttc gctagatacc ccgaccatat gaagcaacac
     4321 gacttcttca agtccgccat gcctgagggc tacgtgcaag agcggaccat cttctttaag
     4381 gacgacggca actacaagac cagggccgaa gtgaagttcg agggcgacac cctggtcaac
     4441 cggatcgagc tgaagggcat cgacttcaaa gaggacggca acatcctggg ccacaagctc
     4501 gagtacaact acaactccca caacgtgtac atcatggccg acaagcagaa aaacggcatc
     4561 aaagtgaact tcaagatcag gcacaacatc gaggacggct ccgtgcagct ggctgatcac
     4621 taccagcaga acacccctat cggcgacgga cctgtgctgc tgcctgataa ccactacctg
     4681 tcctaccagt ccaagctgag caaggacccc aacgagaagc gggaccacat ggtgctgctg
     4741 gaatttgtga ccgccgctgg catcaccctc ggcatggatg agctgtacaa gtccggcaag
     4801 cttagccatg gcttcccgcc ggaggtggag gagcaggatg atggcacgct gcccatgtct
     4861 tgtgcccagg agagcgggat ggaccgtcac cctgcagcct gtgcttctgc taggatcaat
     4921 gtgtgagcta gcgaattcga atttaaatcg gcctcgactg tgccttctag ttgccagcca
     4981 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     5041 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     5101 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     5161 ggggacgcgg ccgcgggccc attggtatgg ctttttcccc gtatcccccc aggtgtctgc
     5221 aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg
     5281 cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg
     5341 gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg
     5401 ggctttgggg gggggctgtc cctgatatct ataacaagaa aatatatata taataagtta
     5461 tcacgtaagt agaacatgaa ataacaatat aattatcgta tgagttaaat cttaaaagtc
     5521 acgtaaaaga taatcatgcg tcattttgac tcacgcggtc gttatagttc aaaatcagtg
     5581 acacttaccg cattgacaag cacgcctcac gggagctcca agcggcgact gagatgtcct
     5641 aaatgcacag cgacggattc gcgctattta gaaagagaga gcaatatttc aagaatgcat
     5701 gcgtcaattt tacgcagact atctttctag ggttaatcta gctgcatcag gatcatatcg
     5761 tcgggtcttt tttccggctc agtcatcgcc caagctggcg ctatctgggc atcggggagg
     5821 aagaagcccg tgccttttcc cgcgaggttg aagcggcatg gaaagagttt gccgaggatg
     5881 actgctgctg cattgacgtt gagcgaaaac gcacgtttac catgatgatt cgggaaggtg
     5941 tggccatgca cgcctttaac ggtgaactgt tcgttcaggc cacctgggat accagttcgt
     6001 cgcggctttt ccggacacag ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca
     6061 ataccggcga cagccggaac tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc
     6121 tgggatatta cgtcagcgag gacgggtatc ctggctggat gccgcagaaa tggacatgga
     6181 taccccgtga gttacccggc gggcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     6241 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6301 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6361 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6421 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6481 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6541 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6601 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6661 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6721 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6781 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6841 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6901 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6961 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7021 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     7081 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7141 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7201 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7261 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7321 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7381 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7441 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7501 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7561 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7621 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7681 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7741 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7801 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7861 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     7921 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     7981 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8041 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8101 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8161 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8221 gacacggaaa tgttgaatac tcat
//
