LOCUS       pCDH-EF1-p27-T2A-Puro   8426 bp ds-DNA     circular     02-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..234
                     /label="RSV Promoter"
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_fwdcolor=#800080
     misc_feature    235..414
                     /label="HIV/LTR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    570..923
                     /label="Gag"
                     /ApEinfo_revcolor=#d70428
                     /ApEinfo_fwdcolor=#d70428
     misc_feature    1079..1311
                     /label="RRE"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1312..1807
                     /label="env"
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_fwdcolor=#0080c0
     misc_feature    1808..1925
                     /label="cPPT"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    1937..2480
                     /label="EF1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2487..3203
                     /label="mVenus"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3240..3821
                     /label="p27K-"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3822..3833
                     /label="p27K-"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3849..3902
                     /label="T2A/peptide"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3909..4508
                     /label="PuroR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4521..5111
                     /label="WPRE"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5183..5416
                     /label="3'LTR"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    5488..5774
                     /label="SV40/EEL/poly(A)s"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    6144..6817
                     /label="PUC/Ori"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6962..7822
                     /label="AmpR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
      481 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
      541 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
      601 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
      661 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
      721 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
      781 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
      841 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
      901 aagtaagacc accgcacagc aagcggccac tgatcttcag acctggagga ggagatatga
      961 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1021 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1081 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1141 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1201 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1261 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1321 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1381 ataaatctct ggaacagaat tggaatcaca cgacctggat ggagtgggac agagaaatta
     1441 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1501 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     1561 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     1621 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     1681 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     1741 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     1801 ggttaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     1861 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa
     1921 ttttatcgat actagtggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     1981 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
     2041 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     2101 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     2161 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     2221 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     2281 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     2341 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     2401 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     2461 aagctgtgac cggcgcctac tctagaATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG
     2521 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG
     2581 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGCTGATC TGCACCACCG
     2641 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GGGCTACGGC CTGCAGTGCT
     2701 TCGCCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG
     2761 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG
     2821 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA
     2881 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT
     2941 ATATCACCGC CGACAAGCAG AAGAACGGCA TCAAGGCCAA CTTCAAGATC CGCCACAACA
     3001 TCGAGGACGG CGGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG
     3061 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCTACCA GTCCAAGCTG AGCAAAGACC
     3121 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC
     3181 TCGGCATGGA CGAGCTGTAC AAGGGATATC CATCACACTG GCGGCCGCTC GAGgccacca
     3241 tgtcaaacgt gagagtgtct aacgggagcc cgagcctgga gcggatggac gccagacaag
     3301 cggagcaccc caagccttcc gcctgcagaa atctcttcgg cccggtcaat catgaagaac
     3361 taacccggga cttggagaag cacTgccggg atatggaaga agcgagtcag cgcaagtgga
     3421 atgccgacgc ccagaatcat aagcccctgg agggcagata cgagtggcag gaggtggaga
     3481 ggggcagctt gcccgagttc tactacaggc ccccgcgccc ccccaagagc gcctgcaagg
     3541 tgctggcgca ggagagccag gatgtcagcg ggagccgcca ggcggtgcct ttaattgggt
     3601 ctcaggcaaa ctctgaggac cggcatttgg tggaccaaat gcctgactcg tcagacaatc
     3661 cggctgggtt agcggagcag tgtccaggga tgaggaagcg acctgctgca gaagattctt
     3721 cttcgcaaaa caaaagggcc aacagaacag aagaaaatgt ttcagacggt tccccgaacg
     3781 ctggcactgt ggagcagacg cccaagaagc ccggccttcg acgccagacg taaggatccg
     3841 cggccgctga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc
     3901 cttccgggat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     3961 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     4021 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     4081 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     4141 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     4201 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     4261 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     4321 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     4381 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     4441 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     4501 gtgcctgaat ctaggtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     4561 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4621 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4681 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4741 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4801 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4861 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4921 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4981 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5041 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5101 cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5161 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg
     5221 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5281 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5341 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5401 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     5461 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     5521 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5581 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     5641 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5701 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     5761 gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt
     5821 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5881 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5941 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     6001 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6061 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6121 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6181 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6241 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6301 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6361 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6421 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6481 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6541 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6601 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     6661 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6721 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6781 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6841 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6901 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6961 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7021 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7081 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7141 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7201 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7261 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7321 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7381 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7441 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7501 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7561 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7621 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7681 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7741 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7801 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7861 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7921 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     7981 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     8041 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     8101 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     8161 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     8221 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     8281 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     8341 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     8401 gtaaaacgac ggccagtgcc aagctg
//