LOCUS       Exported                2538 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Golden Gate ligation product.
ACCESSION   urn.local...ex-du56ev9
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        14..880
                     /label=pFUS3
                     /note="/created_by=koutsoub"
     misc_feature    893..896
                     /label=pYTK001 (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:4tc-dspj8ux"
     terminator      complement(899..1007)
                     /label=CamR Terminator
                     /note="/modified_by=loechner"
     primer_bind     complement(950..970)
                     /label=AA717_pQE-fw
     CDS             complement(1008..1667)
                     /label=CamR
     promoter        complement(1668..1772)
                     /label=CamR Promoter
     rep_origin      complement(1773..2536)
                     /direction=LEFT
                     /label=ColE1
     primer_bind     2467..2484
                     /label=AL41; AL41 (reversed)
ORIGIN
        1 tcggtctcaa acgaacgtgt gatgactact ggacacttca gggcttttcc agatatacag
       61 agctttataa tacaatctaa gggcaaaata aaaaaggaaa aagagtacgg tagttgagaa
      121 aaaaaccgga aaggaattgc tttaaagatc ttagtagaat gaaatagtac cacctactcg
      181 cctccttttt ttgtgcttct tgagaggtct tttgagctgt tgcatcatgc tgttatttag
      241 aggttatttt ttgaccttgg tttttccttc ttttcgccgt actcagcccg agaaaaaagc
      301 agcgactgca ctaaactaga ggaaagaagg cttaaaacat cacgcgatcc atataaagac
      361 ccgagtagtt ggagagattg actacttgac gttcaaaaga acatacataa ggataaattc
      421 gttcggactt tttaacttac acacatcttt tggtgttcat tcaaggggcg gtagtgcttg
      481 tagtccgcac aggcggatac tttttagttt gggaagagtt aactggagag ggcagtttac
      541 tttcacatac tcttttatct ttctgaagta aaatgtgatg tacctacaag caatgcataa
      601 gctcttgagt tttagatctg tttcaccctg gaactcaaaa ttcttttact cgaaattttt
      661 actttttttt tttttgtttc tgcattctct cagattttag atgatgcggt tttttacagg
      721 gcattgaaac aattgcagaa aagcaacata ctaatatatc ataacttttt actcttgcct
      781 ctcagaaact atatatacgt tgtaatcatt ttctttcttc taatagctag ttcgtttgaa
      841 ctacaaggaa ataaggcaga gaaaaagaaa ggaaaataat agatctatgt gagaccagac
      901 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
      961 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     1021 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     1081 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     1141 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     1201 aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc aataaaccct
     1261 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     1321 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     1381 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     1441 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     1501 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     1561 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     1621 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     1681 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     1741 tgaaagttgg aacctcttac gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt
     1801 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1861 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1921 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1981 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2041 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2101 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2161 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2221 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2281 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2341 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2401 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2461 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2521 ttctgtggat aaccgtag
//
