LOCUS       Exported                2029 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Golden Gate ligation product.
ACCESSION   urn.local...fb-du56fa4
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        14..371
                     /label=pMSG5
                     /note="/chromosome=XIV"
                     /note="/modified_by=koutsoub"
                     /note="/mol_type=genomic DNA"
                     /note="/organism=Saccharomyces cerevisiae S288c"
                     /note="/strain=S288c"
                     /db_xref="taxon:559292"
     misc_feature    384..387
                     /label=pYTK001 (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:4sr-dspj8uu"
     terminator      complement(390..498)
                     /label=CamR Terminator
                     /note="/modified_by=loechner"
     primer_bind     complement(441..461)
                     /label=AA717_pQE-fw
     CDS             complement(499..1158)
                     /label=CamR
     promoter        complement(1159..1263)
                     /label=CamR Promoter
     rep_origin      complement(1264..2027)
                     /direction=LEFT
                     /label=ColE1
     primer_bind     1958..1975
                     /label=AL41; AL41 (reversed)
ORIGIN
        1 tcggtctcaa acggtgttaa gaatgtagct ttttcgtcaa agctgggtat ttttcttttt
       61 atagcgcagg agcggaaggc aatttccaaa acaggaatgt actttccaaa aaaggaaaat
      121 gtagtttgtt ttttgatgtt tttttctctt tgactatttc aaatttagaa gtgaaaaaat
      181 acaaaaatgt tacataggat tcgatacagt gtaatatatc attattatta cttttaaata
      241 aagcgtttag taaacaagag tgagggttat gcaactcgtt tagtaaacat tctttttttt
      301 ttcttcattt aagtataaga caacaatagt agcggaagcg atagtaatac atttggtagc
      361 gataagtgca cagatctatg tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc
      421 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
      481 agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
      541 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
      601 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
      661 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
      721 tgaaacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
      781 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
      841 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
      901 atcccatatc accagctcac cgtctttcat tgccatacga aattccggat gagcattcat
      961 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     1021 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     1081 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     1141 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     1201 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat
     1261 caatcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1321 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1381 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1441 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1501 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1561 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1621 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1681 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1741 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1801 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1861 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1921 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1981 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtag
//
