LOCUS       Exported                2103 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Golden Gate ligation product.
ACCESSION   urn.local...g6-du56gsm
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        14..445
                     /label=sst2 promoter
                     /note="/modified_by=koutsoub"
     misc_feature    458..461
                     /label=pYTK001 (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:4sn-dspj8uu"
     terminator      complement(464..572)
                     /label=CamR Terminator
                     /note="/modified_by=loechner"
     primer_bind     complement(515..535)
                     /label=AA717_pQE-fw
     CDS             complement(573..1232)
                     /label=CamR
     promoter        complement(1233..1337)
                     /label=CamR Promoter
     rep_origin      complement(1338..2101)
                     /direction=LEFT
                     /label=ColE1
     primer_bind     2032..2049
                     /label=AL41; AL41 (reversed)
ORIGIN
        1 tcggtctcaa acgattacca aggtcttttg aaagagttcg aacaatacaa agagatggca
       61 actcctttgg aaaagttcaa gttgaaccac ctccgttgtc tctattaatg aagaagccat
      121 gaagtatacg aagttttaac tgaatcatct tcaataatta tgggatgtct gggttttaaa
      181 cgtttcataa tattcaattt tgaaacaatg atcccaatac tgcttttttt ttgactagga
      241 cttggatttg gttgctggtg cgttagaatt gttgtctgtc ctgttatatt tataccggtg
      301 aatgaaatca taatcccagc tctgtcttta cttctcatcg tcctttaact ttggaggtgt
      361 tactgtcgta cgttccttct aggttttgca cgcactatct gaggcgttat aggttcaatt
      421 tggtaattaa agatagagtt gtaagagatc tatgtgagac cagaccaata aaaaacgccc
      481 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
      541 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
      601 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
      661 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
      721 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
      781 acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc
      841 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
      901 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
      961 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc
     1021 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     1081 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     1141 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     1201 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
     1261 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     1321 cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1381 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1441 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1501 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1561 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1621 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1681 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1741 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1801 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1861 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1921 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1981 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2041 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2101 tag
//
