LOCUS       Exported                2097 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...6g-du56d1a
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    5..451
                     /label=Digestion of Candida albicans - Fragment 2
                     extr
                     /label=Digestion of Candida albicans - Fragment 2
                     extraction (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:8e-du56d1d"
     gene            11..442
                     /label=Candida albicans alpha factor
                     
                     /note="/created_by=User"
                     /note="/modified_by=loechner"
     protein_bind    452..455
                     /label=BsmBI
     misc_feature    456..2097
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     ex
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     extraction (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:8e-du56d1d"
     terminator      complement(458..566)
                     /label=CamR Terminator
                     /note="/modified_by=loechner"
     primer_bind     complement(480..497)
                     /label=AA710_CamR-term
     CDS             complement(567..1226)
                     /label=CamR
     promoter        complement(1227..1331)
                     /label=CamR Promoter
     rep_origin      complement(1332..2095)
                     /direction=LEFT
                     /label=ColE1
     primer_bind     2056..2075
                     /label=AA711_ColE1
ORIGIN
        1 tcggtctcat atgaaatttt ctttgacttt gttgactgct acaattgcta ctattgttgc
       61 tgctgcacct gctcaatata ctggtcaagc tattgactca aatcaagttg ttgaaattcc
      121 agaatctgct gtggaagcat atttcccaat tgatgatgaa ttgacgcctg tttttggtga
      181 aatagataat aaaccagtta ttttaattgt caatggtact actttaactt ctggtgctaa
      241 taatgaaaag agagaagcca aatctaaagg tggtttcaga ttgaccaatt ttggctactt
      301 tgagcctggt aaaagggatg ctaacgctga tgcaggattt aggttgacaa atttcggtta
      361 cttcgaacct ggaaaaagag atgccaatgc tgaagctggt tttagattaa caaactttgg
      421 ttattttgaa ccaggtaagt aaggatcctg agaccagacc aataaaaaac gcccggcggc
      481 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
      541 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
      601 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
      661 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
      721 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
      781 ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
      841 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
      901 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
      961 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
     1021 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     1081 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     1141 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     1201 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     1261 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     1321 tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1381 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1441 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1501 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1561 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1621 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1681 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1741 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1801 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1861 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1921 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1981 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2041 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtag
//
