LOCUS       Exported                2184 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...6h-du56d1a
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    5..538
                     /label=Digestion of Eremothecium cymbalariae - Fragmen
                     /label=Digestion of Eremothecium cymbalariae - Fragment 2 
                     extraction (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:8i-du56d1e"
     gene            11..529
                     /label=Eremothecium cymbalariae alpha factor
                     
                     /note="/created_by=User"
                     /note="/modified_by=loechner"
     misc_feature    543..2184
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     ex
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     extraction (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:8i-du56d1e"
     terminator      complement(545..653)
                     /label=CamR Terminator
                     /note="/modified_by=loechner"
     primer_bind     complement(567..584)
                     /label=AA710_CamR-term
     CDS             complement(654..1313)
                     /label=CamR
     promoter        complement(1314..1418)
                     /label=CamR Promoter
     rep_origin      complement(1419..2182)
                     /direction=LEFT
                     /label=ColE1
     primer_bind     2143..2162
                     /label=AA711_ColE1
ORIGIN
        1 tcggtctcat atgaaatttt ataatatttt atctgttgct tctattgcat ctttagtttt
       61 tgctgctcca gtttctgtta atgatgccaa agaaattgct gcaacttttc cacaagaagc
      121 tttattaggt tttttggatt taactgatgc tgaaaatatt gttattttgt ctttggttga
      181 tgaagaaaaa tctggtattg ctttggttaa taaaactatt tgggctactg ctagatctga
      241 acaagctgct ggtatttcta aaagagatgc tgatgcttgg cattggctaa gatttgatag
      301 aggccaacca atccataaaa gaagtgctga tgcagttgca gatgcttggc actggttaag
      361 attcgatcgt ggacaaccaa tacataaaag gtctgcagac gctgttgccg atgcctggca
      421 ttggcttagg tttgataggg gtcagccaat tcacaaaaga tctgctgacg cagtagctga
      481 tgcatggcac tggttgagat ttgacagagg tcaacctatt cataagtaag gatcctgaga
      541 ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
      601 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
      661 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
      721 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
      781 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
      841 aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata
      901 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
      961 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     1021 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     1081 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     1141 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     1201 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     1261 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     1321 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     1381 ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg
     1441 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1501 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1561 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1621 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     1681 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1741 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1801 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1861 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1921 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1981 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2041 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2101 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2161 ccctgattct gtggataacc gtag
//
