LOCUS       Exported                2148 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...6i-du56d1a
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    5..502
                     /label=Digestion of Kazachstania africana - Fragment 2
                     /label=Digestion of Kazachstania africana - Fragment 2 
                     extraction (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:8m-du56d1f"
     gene            11..493
                     /label=Kazachstania africana alpha factor
                     
                     /note="/created_by=User"
                     /note="/modified_by=loechner"
     misc_feature    507..2148
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     ex
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     extraction (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:8m-du56d1f"
     terminator      complement(509..617)
                     /label=CamR Terminator
                     /note="/modified_by=loechner"
     primer_bind     complement(531..548)
                     /label=AA710_CamR-term
     CDS             complement(618..1277)
                     /label=CamR
     promoter        complement(1278..1382)
                     /label=CamR Promoter
     rep_origin      complement(1383..2146)
                     /direction=LEFT
                     /label=ColE1
     primer_bind     2107..2126
                     /label=AA711_ColE1
ORIGIN
        1 tcggtctcat atgaaatttt ctactatttt gtctgctgct gctttggctg cttctgcttt
       61 tgctgctcca attgaacagc cagaacaaga tttaaattct actactattc cagctgaagc
      121 tattatttct tatttggatt tggaaggtga taaagatatt gctgttgttc cattttctaa
      181 tgctactgat tctggtttat tatttgttaa tactacaatt ttagctcaag ctaataaaga
      241 agctggtaca ccattacaaa aaagagaagc agatgctgat gctaatgctt ggcattggtt
      301 aagattatct tatggtcaac caatatacaa gagagatgca aatgcagatg ctgacgctga
      361 tgctgatgcc tggcactggc taagattatc atatggccaa ccgatttata aaagggatgc
      421 taacgctgat gctgatgcag acgctgatgc atggcattgg ttgagattgt catacggtca
      481 acctatttac taaggatcct gagaccagac caataaaaaa cgcccggcgg caaccgagcg
      541 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
      601 gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca
      661 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
      721 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
      781 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct
      841 gaaacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa
      901 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc
      961 cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta
     1021 tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc
     1081 aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc
     1141 tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac
     1201 tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca
     1261 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat
     1321 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc
     1381 aatcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1441 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1501 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1561 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1621 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1681 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1741 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1801 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1861 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1921 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1981 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2041 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2101 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtag
//
