LOCUS       Exported                2091 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...6l-du56d1a
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    5..445
                     /label=Digestion of Lachancea fermentati  - Fragment 2
                     /label=Digestion of Lachancea fermentati  - Fragment 2 
                     extraction (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:8y-du56d1i"
     gene            11..436
                     /label=Lachancea fermentati alpha factor
                     
                     /note="/created_by=User"
                     /note="/modified_by=loechner"
     misc_feature    450..2091
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     ex
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     extraction (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:8y-du56d1i"
     terminator      complement(452..560)
                     /label=CamR Terminator
                     /note="/modified_by=loechner"
     primer_bind     complement(474..491)
                     /label=AA710_CamR-term
     CDS             complement(561..1220)
                     /label=CamR
     promoter        complement(1221..1325)
                     /label=CamR Promoter
     rep_origin      complement(1326..2089)
                     /direction=LEFT
                     /label=ColE1
     primer_bind     2050..2069
                     /label=AA711_ColE1
ORIGIN
        1 tcggtctcat atgcaaagat tattaatttt aattttaggt ttggcttcta ctttaatgtc
       61 tgtttctgct gcaccattaa ctgatgcagc ttctactgtt gatattccac aagaatctat
      121 tttgggtgtt tttgaaattt ctgattatga tgatattgct ttattaccag tttcaaatgg
      181 tactcattct gctgttttga ttttgaatgc tactttgatt gatgctgctg gtacttctaa
      241 ttctgatgaa gttgaaaaaa gatctgcttc tccttggcac tggttaagat taaaaggccc
      301 tatgtacaaa agggatgctt ctccacaagc tgaaccatgg cattggttga gattgaaagg
      361 tccaatgtat aaaagagatg ctaatgccga cgctgatcca tggcattggc taagattgaa
      421 gggtcccatg tattaaggat cctgagacca gaccaataaa aaacgcccgg cggcaaccga
      481 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
      541 caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
      601 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
      661 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
      721 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
      781 gctgaaacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
      841 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
      901 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
      961 ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc
     1021 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
     1081 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
     1141 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
     1201 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     1261 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg
     1321 atcaatcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1381 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1441 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1501 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1561 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1621 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1681 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1741 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1801 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1861 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1921 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1981 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2041 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta g
//
