LOCUS       Exported                2163 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...6o-du56d1a
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    5..517
                     /label=Digestion of S. cerevisiae - Fragment 2 extract
                     /label=Digestion of S. cerevisiae - Fragment 2 extraction 
                     (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:9a-du56d1k"
     gene            11..508
                     /gene="MF(ALPHA)1"
                     /label=Saccharomyces cerevisiae MF(ALPHA)1
                     /note="/SECDrawAs=Gene"
                     /note="/SECStyleId=1"
                     /note="/modified_by=loechner"
     misc_feature    262..265
                     /gene="Kex2 recognition site"
                     /product="Kex cleaves after KR"
                     /label=Kex2 recognition site misc
                     /note="/SECDrawAs=Label"
     misc_feature    522..2163
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     ex
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     extraction (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:9a-du56d1k"
     terminator      complement(524..632)
                     /label=CamR Terminator
                     /note="/modified_by=loechner"
     primer_bind     complement(546..563)
                     /label=AA710_CamR-term
     CDS             complement(633..1292)
                     /label=CamR
     promoter        complement(1293..1397)
                     /label=CamR Promoter
     rep_origin      complement(1398..2161)
                     /direction=LEFT
                     /label=ColE1
     primer_bind     2122..2141
                     /label=AA711_ColE1
ORIGIN
        1 tcggtctcat atgagatttc cttcaatttt tactgcagtt ttattcgcag catcctccgc
       61 attagctgct ccagtcaaca ctacaacaga agatgaaacg gcacaaattc cggctgaagc
      121 tgtcatcggt tacttagatt tagaagggga tttcgatgtt gctgttttgc cattttccaa
      181 cagcacaaat aacgggttat tgtttataaa tactactatt gccagcattg ctgctaaaga
      241 agaaggggta tctttggata aaagagaggc tgaagcttgg cattggttgc aactaaaacc
      301 tggccaacca atgtacaaga gagaagccga agctgaagct tggcattggc tgcaactaaa
      361 gcctggccaa ccaatgtaca aaagagaagc cgacgctgaa gcttggcatt ggctgcaact
      421 aaagcctggc caaccaatgt acaaaagaga agccgacgct gaagcttggc attggttgca
      481 gttaaaaccc ggccaaccaa tgtactaagg atcctgagac cagaccaata aaaaacgccc
      541 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
      601 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
      661 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
      721 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
      781 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
      841 acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc
      901 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
      961 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     1021 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc
     1081 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     1141 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     1201 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     1261 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
     1321 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     1381 cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1441 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1501 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1561 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1621 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1681 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1741 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1801 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1861 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1921 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1981 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2041 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2101 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2161 tag
//
