LOCUS       Exported                2178 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...6p-du56d1a
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    5..532
                     /label=Digestion of Tetrapisispora phaffii - Fragment
                     /label=Digestion of Tetrapisispora phaffii - Fragment 2 
                     extraction (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:9e-du56d1l"
     gene            11..523
                     /label=Tetrapisispora phaffii alpha factor
                     
                     /note="/created_by=User"
                     /note="/modified_by=koutsoub"
     misc_feature    537..2178
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     ex
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     extraction (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:9e-du56d1l"
     terminator      complement(539..647)
                     /label=CamR Terminator
                     /note="/modified_by=loechner"
     primer_bind     complement(561..578)
                     /label=AA710_CamR-term
     CDS             complement(648..1307)
                     /label=CamR
     promoter        complement(1308..1412)
                     /label=CamR Promoter
     rep_origin      complement(1413..2176)
                     /direction=LEFT
                     /label=ColE1
     primer_bind     2137..2156
                     /label=AA711_ColE1
ORIGIN
        1 tcggtctcat atgaaattat catcagtttt atctacttta gctttgactg ctacttcagc
       61 atttgctgct ccagtttcta atgaaagtgt tgataatgct tctgttccag ctgaagctat
      121 tattggttat ttgaattttg atggtgctaa tgatattgct ttattaccat tttcaaattc
      181 aactacttct ggtgttatgt ttattaatac aactattgca gaacaagctt atgaagaagc
      241 tggtgtctct ttgtctaaaa gagaagcaga cgcctggcac tggctgagat tggacccagg
      301 ccaaccattg tacaaaaggg atgctgaagc tgatgcagag gcatggcatt ggctaagatt
      361 agatcctgga caacctttgt ataaaaggga cgcagaagcc gatgccgaag cttggcattg
      421 gttgagattg gatccaggtc agccattata caagagagat gcggaagcag atgctgaagc
      481 ttggcattgg ttaagattag atcctggtca acctttatat taaggatcct gagaccagac
      541 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
      601 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
      661 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
      721 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
      781 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
      841 aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc aataaaccct
      901 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
      961 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     1021 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     1081 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     1141 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     1201 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     1261 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     1321 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     1381 tgaaagttgg aacctcttac gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt
     1441 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1501 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1561 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1621 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1681 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1741 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1801 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1861 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1921 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1981 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2041 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2101 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2161 ttctgtggat aaccgtag
//
