LOCUS       Exported                3075 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...a5-du56dyi
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    5..1429
                     /label=Digestion of Eremothecium cymbalariae Receptor
                     /label=Digestion of Eremothecium cymbalariae Receptor -
                     Fragment 2 extraction (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:cs-du56dyn"
     gene            11..1420
                     /label=E.c. Receptor
                     /note="/created_by=User"
     misc_feature    1434..3075
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     ex
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     extraction (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:cr-du56dyn"
     terminator      complement(1436..1544)
                     /label=CamR Terminator
                     /note="/modified_by=loechner"
     primer_bind     complement(1458..1475)
                     /label=AA710_CamR-term
     CDS             complement(1545..2204)
                     /label=CamR
     promoter        complement(2205..2309)
                     /label=CamR Promoter
     rep_origin      complement(2310..3073)
                     /direction=LEFT
                     /label=ColE1
     primer_bind     3034..3053
                     /label=AA711_ColE1
ORIGIN
        1 tcggtctcat atgtcagagg atactctaat tccaattgag cagtattacg atccatctta
       61 caatccaaga gcttcattat taacttatga atcaatttat aatcataaaa caacagtctc
      121 tttcaaagat atcgaagagt ttattaatac caaaatcaca caaggtgttt ttgttggtac
      181 taaaattggt gctgctggtt tggctattct tgttctttgg atggtttcaa aaaatagaaa
      241 aactccaatt tttttaatta accaagtttc attgttcttg actttgacac attcttcgtt
      301 gtatttgaaa aatttattag gtcatttctc aagtgtaact tttgcattta ccttattccc
      361 acaatatatt tcaaataatg atttacatgt ttatgctgcc accaatatgt ttcaagtctt
      421 cttagttgcc gcaatagaag taagccttat ctttcaggtt agagtcatat ttagagcaga
      481 taactacaag agagtaggca caatactgac atccatctct acagttctgg ggattaccac
      541 tgttgtttta tacttggtta ctgccatcaa ttcaattaag aatgtttact acgatgtttc
      601 taagacgtcc gatgcttatt atttcaacat ttctactata ttgatggcat gttctgttaa
      661 ttttatgact cttttactaa cagtaaaatt gattttggcg ataagatcaa gaagattttt
      721 gggtttaaaa caatttgatt catttcatat tttgttaata atgtctactc aaactttgtt
      781 atgtccatct gttttgtaca ttttagcata tgctttaagt tctagaggta cagattcttt
      841 aacaactatt gctactttgt tggtcactct atctttacca ttgtcatcaa tgtgggctac
      901 atctgctaat aactctacaa aaccatcgag tattaatacc caattctctc catataactg
      961 tgaaactatg tcttttggaa ctggttcttc aacttactat gcaaattcta tgaatagtaa
     1021 taaaagatca ataactaata agttgtatga tttatatcca gcaaaccatt tttcttgtaa
     1081 aagatcatat ggttctggag aagataatac tattgttgat attgaaaaaa aatgttttaa
     1141 tatcggttct tcaattgaaa gttcaccagc tcaaccatca acgttgaaca atggcacttc
     1201 ctctcataat aaatcagtaa atggtaacgt tgaagttcaa acaccaacta ccgcagcaga
     1261 agaagacgct aaaaggtttt ggactgatac atattatcct actggtattc ctgatgaaga
     1321 atttacagat tttaaagttt ctggtgaaca atgtaatatt ttccaatccc cgacatcaaa
     1381 gactagaaac aaatcttatt ctagaaatac aattagatga ggatcctgag accagaccaa
     1441 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     1501 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
     1561 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     1621 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     1681 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     1741 ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta
     1801 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     1861 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     1921 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     1981 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     2041 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     2101 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     2161 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     2221 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     2281 aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc
     2341 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2401 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2461 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2521 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2581 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2641 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2701 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2761 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2821 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2881 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2941 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3001 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3061 tgtggataac cgtag
//
