LOCUS       Exported                3009 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...a9-du56dyj
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    5..1363
                     /label=Digestion of Lachancea fermentati Receptor -
                     Fr
                     /label=Digestion of Lachancea fermentati Receptor -
                     Fragment 2 extraction (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:d9-du56dyq"
     gene            11..1354
                     /label=L.f. Receptor
                     
                     /note="/created_by=User"
     misc_feature    1368..3009
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     ex
                     /label=Digestion of pYTK001 (modified) - Fragment 2
                     extraction (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:d8-du56dyq"
     terminator      complement(1370..1478)
                     /label=CamR Terminator
                     /note="/modified_by=loechner"
     primer_bind     complement(1392..1409)
                     /label=AA710_CamR-term
     CDS             complement(1479..2138)
                     /label=CamR
     promoter        complement(2139..2243)
                     /label=CamR Promoter
     rep_origin      complement(2244..3007)
                     /direction=LEFT
                     /label=ColE1
     primer_bind     2968..2987
                     /label=AA711_ColE1
ORIGIN
        1 tcggtctcat atgacttttg atccaagtct aagctttatt acttatacat ctgcatatgg
       61 ttctgacaca cagttatctt ttgaacaatt ggaatctttg gctcaaacca gactgacaca
      121 aggtattctt tttggtacta ggatcggtgc tgctggtttg gctattattg tcttgtggat
      181 gattacaaaa aacagaaaaa caccaatatt tgttattaac caagtctcat tgtctttctt
      241 gtttcttcat tcagttttat atgttggtta cttattcaga cattatgctt caattattta
      301 caatttaact atgtttactg attttgttcc aagatctgat gtctatttgt acggtgctat
      361 aaatattttc caagttttac ttgtgatttc cattgaagtt tccttaacat accaaattag
      421 agtgattttt aaatcagata attataaaag aattggtaga gttttaatgg tcatttcaat
      481 tgttttggcc gttgtcaccg ctgttatgta cttggttaca gccatcaaat ccatgatcac
      541 catatactca gatatatccg gaaatcctga tacagtattg ttcaatgttt ctacaatatt
      601 actatcttgt tcggtcaatt ttatgacatt aattctcaca tcgaaattgt tgctggctat
      661 cagatccaga agatttttag gtttgaagca attcgattca tttcatattt tgatgattat
      721 gtcttgccaa acactgattt taccttcaat cttttttatc ttggcttacg ctttaaataa
      781 aaatcaggga actgacactt tagcttctgt agcaactttg ttagtgacac tatctttacc
      841 attgagttct atgtgggcta ctgctgcaaa taactcctct tacccaactt ctgttaatac
      901 tcaatattcc tcttcaatgt atgatactga aagtgataga ttcgaaaaac cttctcttta
      961 tactcattct gctaagtctt cagttaactt caaaaaatat caaaatcatc tgtacgatat
     1021 ttatccagtt actagagatc aaatggttga tcatggttgt aatgatgctg atttagaaaa
     1081 gaatcaaaca agaggtacta atttgtcaat aagagatggt gaatcagctc caggtcgtgg
     1141 tgataggtcg gacgttggaa gattagatgt tcctggttat gatttgaacg atgcgtcttc
     1201 tgcctcaaaa gatatgataa atttgaatgt atacactcca aatacattgg atgatgaaga
     1261 agctagagaa ttttggacta ttaatgattc ctcatatcat ccaagattga caaagggctg
     1321 ttctaagaaa tactctggtt ctatcaattc ttgaggatcc tgagaccaga ccaataaaaa
     1381 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     1441 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
     1501 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     1561 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     1621 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     1681 aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa
     1741 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     1801 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     1861 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     1921 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     1981 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     2041 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     2101 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     2161 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     2221 gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca
     2281 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2341 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2401 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2461 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2521 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2581 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2641 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2701 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2761 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2821 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2881 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2941 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3001 taaccgtag
//
