LOCUS       Exported                3426 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...5m-du56bmt
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            11..1771
                     /label=S.c. BAR1
                     /note="/modified_by=loechner"
     primer_bind     complement(629..647)
                     /label=AA103_BAR1-r
     misc_feature    1781..1784
                     /label=Digestion of pYTK001 - Fragment 2 (concatenated
                     /label=Digestion of pYTK001 - Fragment 2 (concatenated
                     sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:5x-du56bmt"
     terminator      complement(1787..1895)
                     /label=CamR Terminator
                     /note="/modified_by=loechner"
     primer_bind     complement(1838..1858)
                     /label=AA717_pQE-fw
     CDS             complement(1896..2555)
                     /label=CamR
     promoter        complement(2556..2660)
                     /label=CamR Promoter
     rep_origin      complement(2661..3424)
                     /direction=LEFT
                     /label=ColE1
     primer_bind     3355..3372
                     /label=AL41; AL41 (reversed)
ORIGIN
        1 tcggtctcat atgtctgcaa ttaatcatct ttgtttgaaa cttattttgg cgagtttcgc
       61 gattattaac accattactg ctttaacaaa cgatggcact ggtcacttag aattcctttt
      121 acaacacgaa gaggagatgt attacgcaac aaccttagat ataggtacac cgtcccaaag
      181 tctgacagtg ttgtttgata ccggatctgc cgatttttgg gttatggatt ctagcaatcc
      241 cttctgctta ccaaattcaa atacgtcatc ctattcaaac gcaacttata atggcgaaga
      301 agttaagcct tcaattgatt gcaggtctat gagtacttat aatgagcata gatcttccac
      361 ctaccaatat ctggaaaatg gtaggtttta catcacatat gctgacggaa catttgctga
      421 cggtagttgg gggacggaaa ctgtatcaat taatggaatt gacatcccca atatccagtt
      481 cggagttgcc aagtatgcta cgacacccgt tagtggtgtt cttggaattg ggtttcctag
      541 aagagagtcc gttaagggct atgaaggtgc tcctaatgaa tattatccta attttcctca
      601 gattttaaaa agtgaaaaaa taatcgatgt ggtcgcgtat tcgctgttct taaactcacc
      661 tgattcaggt actggttcga ttgtttttgg tgccattgat gaatcaaagt tttctggtga
      721 tttgttcact ttccctatgg taaatgaata tcccacaata gtcgacgctc ctgcaacttt
      781 agcaatgact atacaaggat taggtgccca aaacaaaagt agttgtgaac atgaaacgtt
      841 tacgacgacc aagtatccag ttttgttgga ctcaggaacc tcgctattga atgcgcccaa
      901 ggtcatagca gataaaatgg cttcttttgt aaatgcgtcc tatagtgaag aggaaggtat
      961 atatatatta gactgtccag tatctgtagg tgacgtggaa tacaattttg atttcggcga
     1021 tttgcaaata agtgttccac tgtctagttt gattttaagt cccgagacag aaggcagcta
     1081 ttgtgggttt gcggtccagc caacaaacga ttcgatggtt ctgggtgatg tgttcctgtc
     1141 ctctgcatac gtcgtattcg atctcgataa ttataagata tctttagcac aggcaaattg
     1201 gaacgcaagc gaagtttcga aaaagctagt aaatattcaa acagatgggt ctatttcagg
     1261 tgccaaaatt gctacagctg aaccctggtc caccaatgaa ccatttacag tcacctctga
     1321 catttattca tctacaggct gcaagagtag gccttttctt caatcatcga cagcctcttc
     1381 gcttattgca gaaaccaacg tacaaagtcg caactgctct acgaagatgc caggcactag
     1441 atcaactact gtcttaagta agcctactca aaatagtgct atgcatcaaa gtacaggcgc
     1501 tgtcacacaa acctcaaatg aaactaaatt agaattatcc tcgactatgg caaattcggg
     1561 cagtgtctcg cttcccactt cgaattcaat agacaaagag ttcgaacatt cgaaatctca
     1621 aactaccagc gatccaagtg tagcagagca ttctacgttt aaccaaacgt ttgtacatga
     1681 aactaaatat cggcctactc ataagacagt cataacagaa actgtcacga agtattctac
     1741 agtcttaata aatgtctgta aaccaacata tggatcctga gaccagacca ataaaaaacg
     1801 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     1861 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
     1921 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     1981 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     2041 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     2101 ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag
     2161 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     2221 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     2281 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     2341 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     2401 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     2461 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     2521 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     2581 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     2641 cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2701 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2761 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2821 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2881 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2941 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3001 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3061 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3121 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3181 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3241 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3301 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3361 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3421 ccgtag
//
