LOCUS       pRibo_Apex2M__20        5077 bp    DNA        circular     20-SEP-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     pRibo-v5-Apex2 from 1 to 5136
COMMENT     pRibo mpt63-APEX2 from 1 to 5226
COMMENT       
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    60..65
                     /locus_tag="XbaI"
                     /label="XbaI"
                     /ApEinfo_label="XbaI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    61..78
                     /locus_tag="ojcs027"
                     /label="ojcs027"
                     /ApEinfo_label="ojcs027"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    219..224
                     /locus_tag="-35"
                     /label="-35"
                     /ApEinfo_label="-35"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    239..247
                     /locus_tag="-10"
                     /label="-10"
                     /ApEinfo_label="-10"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    335..341
                     /locus_tag="RBS"
                     /label="RBS"
                     /ApEinfo_label="RBS"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    292..329
                     /locus_tag="aptamer"
                     /label="aptamer"
                     /ApEinfo_label="aptamer"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1204..1223
                     /locus_tag="ojcs239"
                     /label="ojcs239"
                     /ApEinfo_label="ojcs239"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1164..1184)
                     /locus_tag="ojcs323"
                     /label="ojcs323"
                     /ApEinfo_label="ojcs323"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(3901..5077,1..33)
                     /locus_tag="oriM"
                     /label="oriM"
                     /ApEinfo_label="oriM"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1204..1223
                     /locus_tag="ojcs330"
                     /label="ojcs330"
                     /ApEinfo_label="ojcs330"
                     /ApEinfo_fwdcolor="#80ffff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(142..161)
                     /locus_tag="ojcs332"
                     /label="ojcs332"
                     /ApEinfo_label="ojcs332"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#ff80ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1140..1157)
                     /locus_tag="ojcs146"
                     /label="ojcs146"
                     /ApEinfo_label="ojcs146"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#80ff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2463..3249
                     /locus_tag="oriE"
                     /label="oriE"
                     /ApEinfo_label="oriE"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3201..3221
                     /locus_tag="ojcs238"
                     /label="ojcs238"
                     /ApEinfo_label="ojcs238"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1360..2175
                     /locus_tag="kanR (aph)"
                     /label="kanR (aph)"
                     /ApEinfo_label="kanR (aph)"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(3292..3900,3254..3271)
                     /locus_tag="oriM(1)"
                     /label="oriM(1)"
                     /ApEinfo_label="oriM"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3272..3291
                     /locus_tag="oriM(2)"
                     /label="oriM(2)"
                     /ApEinfo_label="oriM"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3272..3291
                     /locus_tag="ojcs331"
                     /label="ojcs331"
                     /ApEinfo_label="ojcs331"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    349..351
                     /locus_tag="start codon"
                     /label="start codon"
                     /ApEinfo_label="start codon"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5..24
                     /locus_tag="ojcs297"
                     /label="ojcs297"
                     /ApEinfo_label="ojcs297"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1140..1157
                     /locus_tag="ojcs146r"
                     /label="ojcs146r"
                     /ApEinfo_label="ojcs146r"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    358..1104
                     /locus_tag="Apex2m"
                     /label="Apex2m"
                     /ApEinfo_label="Apex2m"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1218..1244
                     /locus_tag="omlp081r seq primer"
                     /label="omlp081r seq primer"
                     /ApEinfo_label="omlp081r seq primer"
                     /ApEinfo_fwdcolor="#ddfe00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ACTCCGTTGT AGTGCTTGTG GTGGCATCCG TGGCGCGGCC GCGGTACCAG ATCTTTAAAT
       61 CTAGAGGTGa CCACAACGCG CCCGCTTTGA TCGGGGACGT CTGCGGCCGA CCATTTACGG
      121 GTCTTGTTGT CGTTGGCGGT CATGGGCCGA ACATACTCAC CCGGATCGGA GGGCCGAGGA
      181 CAAGGTCGAA CGAGGGGCAT GACCCGGTGC GGGGCTTCTT GCACTCGGCA TAGGCGAGTG
      241 CTAAGAATAA CGTTGGCACT CGCGACCGGG ATACGACTCA CTATAGGTAC CGGTGATACC
      301 AGCATCGTCT TGATGCCCTT GGCAGCACCC TGCTAAGGAG GCAACAAGAT GcatatgGGC
      361 AAGAGCTACC CGACCGTCAG CGCCGACTAC CAGGACGCCG TGGAGAAGGC CAAGAAGAAG
      421 CTGCGCGGCT TCATCGCCGA GAAGCGGTGC GCCCCGCTGA TGCTGCGCCT GGCGTTCCAC
      481 AGCGCGGGCA CCTTCGACAA GGGCACCAAG ACCGGCGGCC CGTTCGGCAC CATCAAGCAC
      541 CCGGCGGAGC TGGCGCACAG CGCCAACAAC GGCCTGGACA TCGCGGTCCG GCTGCTGGAG
      601 CCGCTGAAGG CGGAGTTCCC GATCCTGAGC TACGCCGACT TCTACCAGCT GGCGGGCGTC
      661 GTGGCCGTCG AGGTGACCGG CGGCCCGAAG GTGCCGTTCC ACCCGGGCCG GGAGGACAAG
      721 CCGGAGCCGC CGCCGGAGGG CCGCCTGCCG GACCCGACCA AGGGCTCGGA CCACCTGCGG
      781 GACGTCTTCG GCAAGGCGAT GGGCCTGACC GACCAGGACA TCGTGGCCCT GAGCGGCGGC
      841 CACACCATCG GCGCGGCCCA CAAGGAGCGC AGCGGCTTCG AGGGCCCGTG GACCAGCAAC
      901 CCGCTGATCT TCGACAACTC GTACTTCACC GAGCTGCTGA GCGGCGAGAA GGAAGGCCTG
      961 CTGCAGCTGC CGAGCGACAA GGCGCTGCTG AGCGACCCGG TCTTCCGGCC CCTGGTGGAC
     1021 AAGTACGCGG CCGACGAGGA CGCCTTCTTC GCCGACTACG CCGAGGCCCA CCAGAAGCTG
     1081 TCCGAGCTGG GCTTCGCCGA CGCCaAGCTT ATCGATGTCG ACGTAGTTAA CTAGCGTACG
     1141 ATCGACTGCC AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT
     1201 TATCTGTTGT TTGTCCGGCC ATCATGGCCG CGGTGATCAG CTAGCCAACA AAGCGACGTT
     1261 GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA ATATATCATC ATGAACAATA
     1321 AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT TCAACGGGAA
     1381 ACGTCTTGCT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA
     1441 TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC
     1501 CATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT
     1561 GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT
     1621 ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGGAA AACAGCATTC
     1681 CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC
     1741 CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT
     1801 CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT
     1861 GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA TCTTTTGCCA
     1921 TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC
     1981 GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG
     2041 GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT
     2101 TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC
     2161 GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG
     2221 ACTTGACGGG ACGGCGGCTT TGTTGAATAA ATCGAACTTT TGCTGAGTTG AAGGATCAGA
     2281 TCACGCATCT TCCCGACAAC GCAGACCGTT CCGTGGCAAA GCAAAAGTTC AAAATCACCA
     2341 ACTGGTCCAC CTACAACAAA GCTCTCATCA ACCGTGGCTC CCTCACTTTC TGGCTGGATG
     2401 ATGGGGCGAT TCAGGCCTGG TATGAGTCAG CAACACCTTC TTCACGAGGC AGACCTCACT
     2461 AGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT
     2521 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT
     2581 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA
     2641 TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG
     2701 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA
     2761 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG
     2821 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA
     2881 GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA
     2941 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA
     3001 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT
     3061 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC
     3121 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT
     3181 CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA
     3241 CCGAGCGCAA CGCGTGAGCC CACCAGCTCC GTAAGTTCGG GTGCTGTGTG GCTCGTACCC
     3301 GCGCATTCAG GCGGCAGGGG GTCTAACGGG TCTAAGGCGG CGTGTACGGC CGCCACAGCG
     3361 GCTCTTAGCG GCCCGGAAAC GTCCTCGAAA CGACGCATGT GTTCCTCCTG GTTGGTACAG
     3421 GTGGTTGGGG GTGCTCGGCT GTCGCTGGTG TTTCATCATC AGGGCTCGAC GGGAGAGCGG
     3481 GGGAGTGTGC AGTTGTGGGG TGGCCCCTCA GCGAAATATC TGACTTGGAG CTCGTGTCGG
     3541 ACCATACACC GGTGATTAAT CGTGGTTTAT TATCAAGCGT GAGCCACGTC GCCGACGAAT
     3601 TTGAGCAGCT CTGGCTGCCG TACTGGTCCC TGGCAAGCGA CGATCTGCTC GAGGGGATCT
     3661 ACCGCCAAAG CCGCGCGTCG GCCCTAGGCC GCCGGTACAT CGAGGCGAAC CCAACAGCGC
     3721 TGGCAAACCT GCTGGTCGTG GACGTAGACC ATCCAGACGC AGCGCTCCGA GCGCTCAGCG
     3781 CCCGGGGGTC CCATCCGCTG CCCAACGCGA TCGTGGGCAA TCGCGCCAAC GGCCACGCAC
     3841 ACGCAGTGTG GGCACTCAAC GCCCCTGTTC CACGCACCGA ATACGCGCGG CGTAAGCCGC
     3901 GCCGTCGATG GCGACCGCAG TTACTCAGGC CTCATGACCA AAAACCCCGG CCACATCGCC
     3961 TGGGAAACGG AATGGCTCCA CTCAGATCTC TACACACTCA GCCACATCGA GGCCGAGCTC
     4021 GGCGCGAACA TGCCACCGCC GCGCTGGCGT CAGCAGACCA CGTACAAAGC GGCTCCGACG
     4081 CCGCTAGGGC GGAATTGCGC ACTGTTCGAT TCCGTCAGGT TGTGGGCCTA TCTTCCCGCC
     4141 CTCATGCGGA TCTACCTGCC GACCCGGAAC GTGGACGGAC TCGGCCGCGC GATCTATGCC
     4201 GAGTGCCACG CGCGAAACGC CGAATTTCCG TGCAACGACG TGTGTCCCGG ACCGCTACCG
     4261 GACAGCGAGG TCCGCGCCAT CGCCAACAGC ATTTGGCGTT GGATCACAAC CAAGTCGCGC
     4321 ATTTGGGCGG ACGGGATCGT GGTCTACGAG GCCACACTCA GTGCGCGCCA TGCGGCCATC
     4381 TCGCGGAAGG GCGCAGCAGC GCGCACGGCG GCGAGCACAG TTGCGCGGCG CGCAAAGTCC
     4441 GCGTCAGCCA TGGAGGCATT GCTATGAGCG ACGGCTACAG CGACGGCTAC AGCGACGGCT
     4501 ACAACTGGCA GCCGACTGTC CGCAAAAAGC GGCGCGTGAC CGCCGCCGAA GGCGCTCGAA
     4561 TCACCGGACT ATCCGAACGC CACGTCGTCC GGCTCGTGGC GCAGGAACGC AGCGAGTGGT
     4621 TCGCCGAGCA GGCTGCACGC CGCGAACGCA TCCGCGCCTA TCACGACGAC GAGGGCCACT
     4681 CTTGGCCGCA AACGGCCAAA CATTTCGGGC TGCATCTGGA CACCGTTAAG CGACTCGGCT
     4741 ATCGGGCGAG GAAAGAGCGT GCGGCAGAAC AGGAAGCGGC TCAAAAGGCC CACAACGAAG
     4801 CCGACAATCC ACCGCTGTTC TAACGCAATT GGGGAGCGGG TGTCGCGGGG GTTCCGTGGG
     4861 GGGTTCCGTT GCAACGGGTC GGACAGGTAA AAGTCCTGGT AGACGCTAGT TTTCTGGTTT
     4921 GGGCCATGCC TGTCTCGTTG CGTGTTTCGT TGCGTCCGTT TTGAATACCA GCCAGACGAG
     4981 ACGGGGTTCT ACGAATCTTG GTCGATACCA AGCCATTTCC GCTGAATATC GTGGAGCTCA
     5041 CCGCCAGAAT CGGTGGTTGT GGTGATGTAC GTGGCGA  
//
