LOCUS       pRibo_Sec_APEX2m        5258 bp    DNA        circular     20-SEP-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     pRibo-Sec-Apex2m (200626) seq corrected) from 1 to 5258
COMMENT     pRibo-Sec-Apex2m from 1 to 5170
COMMENT     pRiboMpt63-Apex2M from 1 to 5170
COMMENT     pRibo mpt63-APEX2 from 1 to 5226
COMMENT       
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    56..61
                     /locus_tag="XbaI"
                     /label="XbaI"
                     /ApEinfo_label="XbaI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    57..74
                     /locus_tag="ojcs027"
                     /label="ojcs027"
                     /ApEinfo_label="ojcs027"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    215..220
                     /locus_tag="-35"
                     /label="-35"
                     /ApEinfo_label="-35"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    235..243
                     /locus_tag="-10"
                     /label="-10"
                     /ApEinfo_label="-10"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    331..337
                     /locus_tag="RBS"
                     /label="RBS"
                     /ApEinfo_label="RBS"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    288..325
                     /locus_tag="aptamer"
                     /label="aptamer"
                     /ApEinfo_label="aptamer"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1381..1400
                     /locus_tag="ojcs239"
                     /label="ojcs239"
                     /ApEinfo_label="ojcs239"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1335..1355)
                     /locus_tag="ojcs323"
                     /label="ojcs323"
                     /ApEinfo_label="ojcs323"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(4078..5258,1..29)
                     /locus_tag="oriM"
                     /label="oriM"
                     /ApEinfo_label="oriM"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1381..1400
                     /locus_tag="ojcs330"
                     /label="ojcs330"
                     /ApEinfo_label="ojcs330"
                     /ApEinfo_fwdcolor="#80ffff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(138..157)
                     /locus_tag="ojcs332"
                     /label="ojcs332"
                     /ApEinfo_label="ojcs332"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#ff80ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1311..1328)
                     /locus_tag="ojcs146"
                     /label="ojcs146"
                     /ApEinfo_label="ojcs146"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#80ff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2640..3426
                     /locus_tag="oriE"
                     /label="oriE"
                     /ApEinfo_label="oriE"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3378..3398
                     /locus_tag="ojcs238"
                     /label="ojcs238"
                     /ApEinfo_label="ojcs238"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1537..2352
                     /locus_tag="kanR (aph)"
                     /label="kanR (aph)"
                     /ApEinfo_label="kanR (aph)"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(3469..4077,3431..3448)
                     /locus_tag="oriM(1)"
                     /label="oriM(1)"
                     /ApEinfo_label="oriM"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3449..3468
                     /locus_tag="oriM(2)"
                     /label="oriM(2)"
                     /ApEinfo_label="oriM"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3449..3468
                     /locus_tag="ojcs331"
                     /label="ojcs331"
                     /ApEinfo_label="ojcs331"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    345..347
                     /locus_tag="start codon"
                     /label="start codon"
                     /ApEinfo_label="start codon"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    354..437
                     /locus_tag="mpt63 signal peptide"
                     /label="mpt63 signal peptide"
                     /ApEinfo_label="mpt63 signal peptide"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1..20
                     /locus_tag="ojcs297"
                     /label="ojcs297"
                     /ApEinfo_label="ojcs297"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1311..1328
                     /locus_tag="ojcs146r"
                     /label="ojcs146r"
                     /ApEinfo_label="ojcs146r"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    62..347
                     /locus_tag="Hsp60 promotor theophylline riboswithc"
                     /label="Hsp60 promotor theophylline riboswithc"
                     /ApEinfo_label="Hsp60 promotor theophylline riboswithc"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    354..437
                     /locus_tag="SEC"
                     /label="SEC"
                     /ApEinfo_label="SEC"
                     /ApEinfo_fwdcolor="#ff00b8"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    444..1190
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#ff5000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 CGTTGTAGTG CTTGTGGTGG CATCCGTGGC GCGGCCGCGG TACCAGATCT TTAAATCTAG
       61 AGGTGaCCAC AACGCGCCCG CTTTGATCGG GGACGTCTGC GGCCGACCAT TTACGGGTCT
      121 TGTTGTCGTT GGCGGTCATG GGCCGAACAT ACTCACCCGG ATCGGAGGGC CGAGGACAAG
      181 GTCGAACGAG GGGCATGACC CGGTGCGGGG CTTCTTGCAC TCGGCATAGG CGAGTGCTAA
      241 GAATAACGTT GGCACTCGCG ACCGGGATAC GACTCACTAT AGGTACCGGT GATACCAGCA
      301 TCGTCTTGAT GCCCTTGGCA GCACCCTGCT AAGGAGGCAA CAAGATGcat atgAAGCTCA
      361 CCACAATGAT CAAGACGGCA GTAGCGGTCG TGGCCATGGC GGCCATCGCG ACCTTTGCGG
      421 CACCGGTCGC GTTGGCTgga tccGGCAAGA GCTACCCGAC CGTCAGCGCC GACTACCAGG
      481 ACGCCGTGGA GAAGGCCAAG AAGAAGCTGC GCGGCTTCAT CGCCGAGAAG CGGTGCGCCC
      541 CGCTGATGCT GCGCCTGGCG TTCCACAGCG CGGGCACCTT CGACAAGGGC ACCAAGACCG
      601 GCGGCCCGTT CGGCACCATC AAGCACCCGG CGGAGCTGGC GCACAGCGCC AACAACGGCC
      661 TGGACATCGC GGTCCGGCTG CTGGAGCCGC TGAAGGCGGA GTTCCCGATC CTGAGCTACG
      721 CCGACTTCTA CCAGCTGGCG GGCGTCGTGG CCGTCGAGGT GACCGGCGGC CCGAAGGTGC
      781 CGTTCCACCC GGGCCGGGAG GACAAGCCGG AGCCGCCGCC GGAGGGCCGC CTGCCGGACC
      841 CGACCAAGGG CTCGGACCAC CTGCGGGACG TCTTCGGCAA GGCGATGGGC CTGACCGACC
      901 AGGACATCGT GGCCCTGAGC GGCGGCCACA CCATCGGCGC GGCCCACAAG GAGCGCAGCG
      961 GCTTCGAGGG CCCGTGGACC AGCAACCCGC TGATCTTCGA CAACTCGTAC TTCACCGAGC
     1021 TGCTGAGCGG CGAGAAGGAA GGCCTGCTGC AGCTGCCGAG CGACAAGGCG CTGCTGAGCG
     1081 ACCCGGTCTT CCGGCCCCTG GTGGACAAGT ACGCGGCCGA CGAGGACGCC TTCTTCGCCG
     1141 ACTACGCCGA GGCCCACCAG AAGCTGTCCG AGCTGGGCTT CGCCGACGCC AAGCTTtgaA
     1201 TCGatacatc aggaattcgc tagcgactac aaggacgacg acggcgatta taaagatcat
     1261 gacaTCGACt acaaggacga cgacgacaag tagGTAGTTA ACTAGCGTAC GATCGACTGC
     1321 CAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTAC ATCAGGGCCT TTCGTTTTAT
     1381 CTGTTGTTTG TCCGGCCATC ATGGCCGCGG TGATCAGCTA GCCAACAAAG CGACGTTGTG
     1441 TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAAATA TATCATCATG AACAATAAAA
     1501 CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA ACGGGAAACG
     1561 TCTTGCTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG
     1621 GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCCAT
     1681 GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG
     1741 ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC
     1801 CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGGAAAAC AGCATTCCAG
     1861 GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG
     1921 CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT
     1981 CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC
     2041 GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAATCT TTTGCCATTC
     2101 TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG
     2161 GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT
     2221 CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT
     2281 CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT
     2341 GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT
     2401 TGACGGGACG GCGGCTTTGT TGAATAAATC GAACTTTTGC TGAGTTGAAG GATCAGATCA
     2461 CGCATCTTCC CGACAACGCA GACCGTTCCG TGGCAAAGCA AAAGTTCAAA ATCACCAACT
     2521 GGTCCACCTA CAACAAAGCT CTCATCAACC GTGGCTCCCT CACTTTCTGG CTGGATGATG
     2581 GGGCGATTCA GGCCTGGTAT GAGTCAGCAA CACCTTCTTC ACGAGGCAGA CCTCACTAGT
     2641 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC
     2701 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC
     2761 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC
     2821 CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC
     2881 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT
     2941 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT
     3001 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT
     3061 ACCTACAGCG TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT
     3121 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG
     3181 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT
     3241 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT
     3301 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG
     3361 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG
     3421 AGCGCAACGC GTGAGCCCAC CAGCTCCGTA AGTTCGGGTG CTGTGTGGCT CGTACCCGCG
     3481 CATTCAGGCG GCAGGGGGTC TAACGGGTCT AAGGCGGCGT GTACGGCCGC CACAGCGGCT
     3541 CTTAGCGGCC CGGAAACGTC CTCGAAACGA CGCATGTGTT CCTCCTGGTT GGTACAGGTG
     3601 GTTGGGGGTG CTCGGCTGTC GCTGGTGTTT CATCATCAGG GCTCGACGGG AGAGCGGGGG
     3661 AGTGTGCAGT TGTGGGGTGG CCCCTCAGCG AAATATCTGA CTTGGAGCTC GTGTCGGACC
     3721 ATACACCGGT GATTAATCGT GGTTTATTAT CAAGCGTGAG CCACGTCGCC GACGAATTTG
     3781 AGCAGCTCTG GCTGCCGTAC TGGTCCCTGG CAAGCGACGA TCTGCTCGAG GGGATCTACC
     3841 GCCAAAGCCG CGCGTCGGCC CTAGGCCGCC GGTACATCGA GGCGAACCCA ACAGCGCTGG
     3901 CAAACCTGCT GGTCGTGGAC GTAGACCATC CAGACGCAGC GCTCCGAGCG CTCAGCGCCC
     3961 GGGGGTCCCA TCCGCTGCCC AACGCGATCG TGGGCAATCG CGCCAACGGC CACGCACACG
     4021 CAGTGTGGGC ACTCAACGCC CCTGTTCCAC GCACCGAATA CGCGCGGCGT AAGCCGCGCC
     4081 GTCGATGGCG ACCGCAGTTA CTCAGGCCTC ATGACCAAAA ACCCCGGCCA CATCGCCTGG
     4141 GAAACGGAAT GGCTCCACTC AGATCTCTAC ACACTCAGCC ACATCGAGGC CGAGCTCGGC
     4201 GCGAACATGC CACCGCCGCG CTGGCGTCAG CAGACCACGT ACAAAGCGGC TCCGACGCCG
     4261 CTAGGGCGGA ATTGCGCACT GTTCGATTCC GTCAGGTTGT GGGCCTATCT TCCCGCCCTC
     4321 ATGCGGATCT ACCTGCCGAC CCGGAACGTG GACGGACTCG GCCGCGCGAT CTATGCCGAG
     4381 TGCCACGCGC GAAACGCCGA ATTTCCGTGC AACGACGTGT GTCCCGGACC GCTACCGGAC
     4441 AGCGAGGTCC GCGCCATCGC CAACAGCATT TGGCGTTGGA TCACAACCAA GTCGCGCATT
     4501 TGGGCGGACG GGATCGTGGT CTACGAGGCC ACACTCAGTG CGCGCCATGC GGCCATCTCG
     4561 CGGAAGGGCG CAGCAGCGCG CACGGCGGCG AGCACAGTTG CGCGGCGCGC AAAGTCCGCG
     4621 TCAGCCATGG AGGCATTGCT ATGAGCGACG GCTACAGCGA CGGCTACAGC GACGGCTACA
     4681 ACTGGCAGCC GACTGTCCGC AAAAAGCGGC GCGTGACCGC CGCCGAAGGC GCTCGAATCA
     4741 CCGGACTATC CGAACGCCAC GTCGTCCGGC TCGTGGCGCA GGAACGCAGC GAGTGGTTCG
     4801 CCGAGCAGGC TGCACGCCGC GAACGCATCC GCGCCTATCA CGACGACGAG GGCCACTCTT
     4861 GGCCGCAAAC GGCCAAACAT TTCGGGCTGC ATCTGGACAC CGTTAAGCGA CTCGGCTATC
     4921 GGGCGAGGAA AGAGCGTGCG GCAGAACAGG AAGCGGCTCA AAAGGCCCAC AACGAAGCCG
     4981 ACAATCCACC GCTGTTCTAA CGCAATTGGG GAGCGGGTGT CGCGGGGGTT CCGTGGGGGG
     5041 TTCCGTTGCA ACGGGTCGGA CAGGTAAAAG TCCTGGTAGA CGCTAGTTTT CTGGTTTGGG
     5101 CCATGCCTGT CTCGTTGCGT GTTTCGTTGC GTCCGTTTTG AATACCAGCC AGACGAGACG
     5161 GGGTTCTACG AATCTTGGTC GATACCAAGC CATTTCCGCT GAATATCGTG GAGCTCACCG
     5221 CCAGAATCGG TGGTTGTGGT GATGTACGTG GCGAACTC  
//
