LOCUS       pMV306_pRibo_SEC        5086 bp    DNA        circular     17-SEP-2021
DEFINITION  integrating mycobacterial complementation vector.
SOURCE      
  ORGANISM  
COMMENT     pMV306 KnR (corrected) from 1 to 3981
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             1958..2979
                     /vntifkey="4"
                     /locus_tag="int"
                     /label="int"
                     /ApEinfo_label="int"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_marker     3246..4061
                     /vntifkey="22"
                     /note="aph"
                     /locus_tag="Kan"
                     /label="Kan"
                     /ApEinfo_label="Kan"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    87..99
                     /locus_tag="ojcs027"
                     /label="ojcs027"
                     /ApEinfo_label="ojcs027"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             4325..4725
                     /vntifkey="4"
                     /locus_tag="oriE"
                     /label="oriE"
                     /ApEinfo_label="oriE"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    240..245
                     /locus_tag="-35"
                     /label="-35"
                     /ApEinfo_label="-35"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3064..3083
                     /vntifkey="28"
                     /locus_tag="kan\for\2"
                     /label="kan\for\2"
                     /ApEinfo_label="kan\for\2"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    260..268
                     /locus_tag="-10"
                     /label="-10"
                     /ApEinfo_label="-10"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(163..182)
                     /locus_tag="ojcs332"
                     /label="ojcs332"
                     /ApEinfo_label="ojcs332"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#ff80ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1..21
                     /locus_tag="ojcs238"
                     /label="ojcs238"
                     /ApEinfo_label="ojcs238"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    356..362
                     /locus_tag="RBS"
                     /label="RBS"
                     /ApEinfo_label="RBS"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1323..1323)
                     /locus_tag="ojcs239"
                     /label="ojcs239"
                     /ApEinfo_label="ojcs239"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    313..350
                     /locus_tag="aptamer"
                     /label="aptamer"
                     /ApEinfo_label="aptamer"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1318..1322
                     /locus_tag="mismatch with ojcs239 (invalid primer)"
                     /label="mismatch with ojcs239 (invalid primer)"
                     /ApEinfo_label="mismatch with ojcs239 (invalid primer)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1631..1700
                     /vntifkey="4"
                     /locus_tag="attP"
                     /label="attP"
                     /ApEinfo_label="attP"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    370..372
                     /locus_tag="start codon"
                     /label="start codon"
                     /ApEinfo_label="start codon"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    379..462
                     /locus_tag="mpt63 signal peptide"
                     /label="mpt63 signal peptide"
                     /ApEinfo_label="mpt63 signal peptide"
                     /ApEinfo_fwdcolor="#ff0001"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1303..1323
                     /locus_tag="oevv137"
                     /label="oevv137"
                     /ApEinfo_label="oevv137"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    469..1224
                     /locus_tag="Apex2m"
                     /label="Apex2m"
                     /ApEinfo_label="Apex2m"
                     /ApEinfo_fwdcolor="#ff259b"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    87..372
                     /locus_tag="pRibo"
                     /label="pRibo"
                     /ApEinfo_label="pRibo"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAA CGCGTGCGGC
       61 CGCGGTACCA GATCTTTAAA TCTAGAGGTG aCCACAACGC GCCCGCTTTG ATCGGGGACG
      121 TCTGCGGCCG ACCATTTACG GGTCTTGTTG TCGTTGGCGG TCATGGGCCG AACATACTCA
      181 CCCGGATCGG AGGGCCGAGG ACAAGGTCGA ACGAGGGGCA TGACCCGGTG CGGGGCTTCT
      241 TGCACTCGGC ATAGGCGAGT GCTAAGAATA ACGTTGGCAC TCGCGACCGG GATACGACTC
      301 ACTATAGGTA CCGGTGATAC CAGCATCGTC TTGATGCCCT TGGCAGCACC CTGCTAAGGA
      361 GGCAACAAGA TGcatatgAA GCTCACCACA ATGATCAAGA CGGCAGTAGC GGTCGTGGCC
      421 ATGGCGGCCA TCGCGACCTT TGCGGCACCG GTCGCGTTGG CTggatccGG CAAGAGCTAC
      481 CCGACCGTCA GCGCCGACTA CCAGGACGCC GTGGAGAAGG CCAAGAAGAA GCTGCGCGGC
      541 TTCATCGCCG AGAAGCGGTG CGCCCCGCTG ATGCTGCGCC TGGCGTTCCA CAGCGCGGGC
      601 ACCTTCGACA AGGGCACCAA GACCGGCGGC CCGTTCGGCA CCATCAAGCA CCCGGCGGAG
      661 CTGGCGCACA GCGCCAACAA CGGCCTGGAC ATCGCGGTCC GGCTGCTGGA GCCGCTGAAG
      721 GCGGAGTTCC CGATCCTGAG CTACGCCGAC TTCTACCAGC TGGCGGGCGT CGTGGCCGTC
      781 GAGGTGACCG GCGGCCCGAA GGTGCCGTTC CACCCGGGCC GGGAGGACAA GCCGGAGCCG
      841 CCGCCGGAGG GCCGCCTGCC GGACCCGACC AAGGGCTCGG ACCACCTGCG GGACGTCTTC
      901 GGCAAGGCGA TGGGCCTGAC CGACCAGGAC ATCGTGGCCC TGAGCGGCGG CCACACCATC
      961 GGCGCGGCCC ACAAGGAGCG CAGCGGCTTC GAGGGCCCGT GGACCAGCAA CCCGCTGATC
     1021 TTCGACAACT CGTACTTCAC CGAGCTGCTG AGCGGCGAGA AGGAAGGCCT GCTGCAGCTG
     1081 CCGAGCGACA AGGCGCTGCT GAGCGACCCG GTCTTCCGGC CCCTGGTGGA CAAGTACGCG
     1141 GCCGACGAGG ACGCCTTCTT CGCCGACTAC GCCGAGGCCC ACCAGAAGCT GTCCGAGCTG
     1201 GGCTTCGCCG ACGCCAAGCT TTGAATCGAT GTCGACGTAG TTAACTAGCG TACGATCGAC
     1261 TGCCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATGCC
     1321 ATCATGGCCG CGGTGATCAG CTAGAGCCGT GAACGACAGG GCGAACGCCA GCCCGCCGAC
     1381 GGCGAGGGTT CCGACCGCTG CAACTCCCGG TGCAACCTTG TCCCGGTCTA TTCTCTTCAC
     1441 TGCACCAGCT CCAATCTGGT GTGAATGCCC CTCGTCTGTT CGCGCAGGCG GGGGGCTCTA
     1501 TTCGTTTGTC AGCATCGAAA GTAGCCAGAT CAGGGATGCG TTGCAACCGC GTATGCCCAG
     1561 GTCAGAAGAG TCGCACAAGA GTTGCAGACC CCTGGAAAGA AAAATGGCCA GAGGGCGAAA
     1621 ACACCCTCTG ACCAGCGGAG CGGGCGACGG GAATCGAACC CGCGTAGCTA GTTTGGAAGA
     1681 ATGGGTGTCT GCCGACCACA TATATGGGCC GGTCAAGATA GGTTTTTACC CCCTCTCGGC
     1741 TGCATCCTCT AAGTGGAAAG AAATTGCAGG TCGTAGAAGC GCGTTGAAGC CTGAGAGTTG
     1801 CACAGGAGTT GCAACCCGGT AGCCTTGTTC ACGACGAGAG GAGACCTAGT TGGCACGTCG
     1861 CGGATGGGGA TCGCTGAAGA CTCAGCGCAG CGGGAGGATC CAAGCCTCAT ACGTCAACCC
     1921 GCAGGACGGT GTGAGGTACT ACGCGCTGCA GACCTACGAC AACAAGATGG ACGCCGAAGC
     1981 CTGGCTCGCG GGCGAGAAGC GGCTCATCGA GATGGAGACC TGGACCCCTC CACAGGACCG
     2041 GGCGAAGAAG GCAGCCGCCA GCGCCATCAC GCTGGAGGAG TACACCCGGA AGTGGCTCGT
     2101 GGAGCGCGAC CTCGCAGACG GCACCAGGGA TCTGTACAGC GGGCACGCGG AGCGCCGCAT
     2161 CTACCCGGTG CTAGGTGAAG TGGCGGTCAC AGAGATGACG CCAGCTCTGG TGCGTGCGTG
     2221 GTGGGCCGGG ATGGGTAGGA AGCACCCGAC TGCCCGCCGG CATGCCTACA ACGTCCTCCG
     2281 GGCGGTGATG AACACAGCGG TCGAGGACAA GCTGATCGCA GAGAACCCGT GCCGGATCGA
     2341 GCAGAAGGCA GCCGATGAGC GCGACGTAGA GGCGCTGACG CCTGAGGAGC TGGACATCGT
     2401 CGCCGCTGAG ATCTTCGAGC ACTACCGGAT CGCGGCATAC ATCCTGGCGT GGACGAGCCT
     2461 CCGGTTCGGA GAGCTGATCG AGCTTCGCCG CAAGGACATC GTGGACGACG GCATGACGAT
     2521 GAAGCTCCGG GTGCGCCGTG GCGCTTCCCG CGTGGGGAAC AAGATCGTCG TTGGCAACGC
     2581 CAAGACCGTC CGGTCGAAGC GTCCTGTGAC GGTTCCGCCT CACGTCGCGG AGATGATCCG
     2641 AGCGCACATG AAGGACCGTA CGAAGATGAA CAAGGGCCCC GAGGCATTCC TGGTGACCAC
     2701 GACGCAGGGC AACCGGCTGT CGAAGTCCGC GTTCACCAAG TCGCTGAAGC GTGGCTACGC
     2761 CAAGATCGGT CGGCCGGAAC TCCGCATCCA CGACCTCCGC GCTGTCGGCG CTACGTTCGC
     2821 CGCTCAGGCA GGTGCGACGA CCAAGGAGCT GATGGCCCGT CTCGGTCACA CGACTCCTAG
     2881 GATGGCGATG AAGTACCAGA TGGCGTCTGA GGCCCGCGAC GAGGCTATCG CTGAGGCGAT
     2941 GTCCAAGCTG GCCAAGACCT CCTGAAACGC AAAAAGCCCC CCTCCCAAGG ACACTGAGTC
     3001 CTAAAGAGGG GGGTTTCTTG TCAGTACGCG AAGAACCACG CCTGGCCGCG AGCGCCAGCA
     3061 CCGCCGCTCT GTGCGGAGAC CTGGGCACCA GCCCCGCCGC CGCCAGGAGC ATTGCCGTTC
     3121 CCGCCAGCTA GCAACAAAGC GACGTTGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG
     3181 ATAAAAATAT ATCATCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG
     3241 GTGTTATGAG CCATATTCAA CGGGAAACGT CTTGCTCGAG GCCGCGATTA AATTCCAACA
     3301 TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA
     3361 CAATCTATCG CTTGTATGGG AAGCCCCATG CGCCAGAGTT GTTTCTGAAA CATGGCAAAG
     3421 GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG ACGGAATTTA
     3481 TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG TTACTCACCA
     3541 CTGCGATCCC CGGGAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT TCAGGTGAAA
     3601 ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT GTTTGTAATT
     3661 GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA ATGAATAACG
     3721 GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT GAACAAGTCT
     3781 GGAAAGAAAT GCATAATCTT TTGCCATTCT CACCGGATTC AGTCGTCACT CATGGTGATT
     3841 TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT GATGTTGGAC
     3901 GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC CTCGGTGAGT
     3961 TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT CCTGATATGA
     4021 ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA ATCAGAATTG GTTAATTGGT
     4081 TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG CGGCTTTGTT GAATAAATCG
     4141 AACTTTTGCT GAGTTGAAGG ATCAGATCAC GCATCTTCCC GACAACGCAG ACCGTTCCGT
     4201 GGCAAAGCAA AAGTTCAAAA TCACCAACTG GTCCACCTAC AACAAAGCTC TCATCAACCG
     4261 TGGCTCCCTC ACTTTCTGGC TGGATGATGG GGCGATTCAG GCCTGGTATG AGTCAGCAAC
     4321 ACCTTCTTCA CGAGGCAGAC CTCACTAGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT
     4381 CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA
     4441 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA
     4501 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT
     4561 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT
     4621 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA
     4681 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT
     4741 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC
     4801 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA
     4861 GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG
     4921 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA
     4981 AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT
     5041 GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACC 
//
