LOCUS       pTAG1                   2638 bp ds-DNA     circular     11-SEP-2021
SOURCE      
  ORGANISM  
COMMENT     pTAG1
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1998..2612
                     /vntifkey="21"
                     /locus_tag="rep\-\pMB1"
                     /ApEinfo_label="rep\-\pMB1"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             658..1665
                     /vntifkey="4"
                     /locus_tag="spec\orf?"
                     /ApEinfo_label="spec\orf?"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    251..526
                     /vntifkey="21"
                     /locus_tag="lacZ'"
                     /ApEinfo_label="lacZ'"
                     /ApEinfo_fwdcolor="#333333"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        129..250
                     /vntifkey="30"
                     /locus_tag="lacZ\promoter"
                     /ApEinfo_label="lacZ\promoter"
                     /ApEinfo_fwdcolor="#933500"
                     /ApEinfo_revcolor="#933500"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(582..587)
                     /locus_tag="BsaI Restriction Enzyme Binding Site"
                     /ApEinfo_label="BsaI Restriction Enzyme Binding Site"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    12..88
                     /locus_tag="tRNA"
                     /ApEinfo_label="tRNA"
                     /ApEinfo_fwdcolor="#80003f"
                     /ApEinfo_revcolor="#80003f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    561..580
                     /locus_tag="sgRNA scaffold"
                     /ApEinfo_label="sgRNA scaffold"
                     /ApEinfo_fwdcolor="#073f80"
                     /ApEinfo_revcolor="#073f80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1933..2615
                     /locus_tag="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             458..526
                     /locus_tag="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    211..233
                     /locus_tag="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    554..559
                     /locus_tag="Esp3I recognition site"
                     /ApEinfo_label="Esp3I recognition site"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(90..95)
                     /locus_tag="Esp3I recognition site(1)"
                     /ApEinfo_label="Esp3I recognition site"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1..6
                     /locus_tag="BsaI Binding site"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(582..587)
                     /locus_tag="BsaI Binding site(1)"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ggtctcaAAT Gaacaaagca ccagtggtct agtggtagaa tagtaccctg ccacggtaca
       61 gacccgggtt cgattcccgg ctggTGCAtg agacgagatc tagacgaccg gtggaaagcg
      121 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
      181 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
      241 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
      301 aagctggagg acctcgaggg ggggcccggt acccaattcg ccctatagtg agtcgtatta
      361 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      421 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      481 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
      541 cggcgccact agtcgtctcc GTTTtagagc tagaaatagc cgagaccacg aagtgccgag
      601 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
      661 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
      721 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
      781 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
      841 cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt gatcgccgaa
      901 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
      961 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt
     1021 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     1081 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     1141 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     1201 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     1261 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     1321 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     1381 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     1441 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     1501 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     1561 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     1621 tttgtccatt acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctagctaga
     1681 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa
     1741 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     1801 gcataataag ccctacacaa attgggagat atatcatgct gtcagaccaa gtttactcat
     1861 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1921 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1981 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2041 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2101 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2161 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2221 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2281 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2341 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2401 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2461 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2521 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2581 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg cactctgt
//
