LOCUS       pTAG4                   2706 bp ds-DNA     circular     11-SEP-2021
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             727..1734
                     /vntifkey="4"
                     /locus_tag="spec\orf?"
                     /ApEinfo_label="spec\orf?"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2067..2681
                     /vntifkey="21"
                     /locus_tag="rep\-\pMB1"
                     /ApEinfo_label="rep\-\pMB1"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    318..593
                     /vntifkey="21"
                     /locus_tag="lacZ'"
                     /ApEinfo_label="lacZ'"
                     /ApEinfo_fwdcolor="#333333"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        196..317
                     /vntifkey="30"
                     /locus_tag="lacZ\promoter"
                     /ApEinfo_label="lacZ\promoter"
                     /ApEinfo_fwdcolor="#933500"
                     /ApEinfo_revcolor="#933500"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      2002..2684
                     /locus_tag="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             525..593
                     /locus_tag="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    278..300
                     /locus_tag="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(651..656)
                     /locus_tag="BsaI Restriction Enzyme Binding Site"
                     /ApEinfo_label="BsaI Restriction Enzyme Binding Site"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    621..626
                     /locus_tag="Esp3I recognition site"
                     /ApEinfo_label="Esp3I recognition site"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(157..162)
                     /locus_tag="Esp3I recognition site(1)"
                     /ApEinfo_label="Esp3I recognition site"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2..7
                     /locus_tag="BsaI Restriction Enzyme Binding Site(1)"
                     /ApEinfo_label="BsaI Restriction Enzyme Binding Site"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    9..78
                     /locus_tag="sgRNA scaffold"
                     /ApEinfo_label="sgRNA scaffold"
                     /ApEinfo_fwdcolor="#073f80"
                     /ApEinfo_revcolor="#073f80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    79..155
                     /locus_tag="tRNA"
                     /ApEinfo_label="tRNA"
                     /ApEinfo_fwdcolor="#80003f"
                     /ApEinfo_revcolor="#80003f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    628..649
                     /locus_tag="sgRNA scaffold(1)"
                     /ApEinfo_label="sgRNA scaffold"
                     /ApEinfo_fwdcolor="#073f80"
                     /ApEinfo_revcolor="#073f80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tggtctcaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
       61 gtggcaccga gtcggtgcaa caaagcacca gtggtctagt ggtagaatag taccctgcca
      121 cggtacagac ccgggttcga ttcccggctg gTGCAtGAGA cgagatctag acgaccggtg
      181 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      241 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      301 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
      361 ggaacaaaag ctggaggacc tcgagggggg gcccggtacc caattcgccc tatagtgagt
      421 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
      481 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
      541 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
      601 cctgtagcgg cgccactagt cgtctccGTT Ttagagctag aaatagcaac gagaccacga
      661 agtgccgagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
      721 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      781 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
      841 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
      901 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg
      961 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     1021 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     1081 agcgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     1141 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     1201 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     1261 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     1321 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     1381 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     1441 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     1501 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     1561 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     1621 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     1681 ttggaagaat ttgtccatta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt
     1741 ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     1801 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     1861 tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgctg tcagaccaag
     1921 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1981 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2041 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2101 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2161 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2221 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2281 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2341 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2401 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2461 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2521 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2581 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2641 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgc
     2701 actctg     
//
