LOCUS       pTAG5                   2713 bp ds-DNA     circular     11-SEP-2021
SOURCE      
  ORGANISM  
COMMENT     New DNA from 1 to 2659
COMMENT     pAGM9121 from 1 to 2659
COMMENT     
COMMENT     No SpeI, BglII, or RV sites in it.
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             733..1740
                     /vntifkey="4"
                     /locus_tag="spec\orf?"
                     /ApEinfo_label="spec\orf?"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2073..2687
                     /vntifkey="21"
                     /locus_tag="rep\-\pMB1"
                     /ApEinfo_label="rep\-\pMB1"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    305..580
                     /vntifkey="21"
                     /locus_tag="lacZ'"
                     /ApEinfo_label="lacZ'"
                     /ApEinfo_fwdcolor="#333333"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        183..304
                     /vntifkey="30"
                     /locus_tag="lacZ\promoter"
                     /ApEinfo_label="lacZ\promoter"
                     /ApEinfo_fwdcolor="#933500"
                     /ApEinfo_revcolor="#933500"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      2008..2690
                     /locus_tag="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             512..580
                     /locus_tag="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    265..287
                     /locus_tag="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(657..662)
                     /locus_tag="BsaI Restriction Enzyme Binding Site"
                     /ApEinfo_label="BsaI Restriction Enzyme Binding Site"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    608..613
                     /locus_tag="Esp3I recognition site"
                     /ApEinfo_label="Esp3I recognition site"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(144..149)
                     /locus_tag="Esp3I recognition site(1)"
                     /ApEinfo_label="Esp3I recognition site"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1..6
                     /locus_tag="BsaI Restriction Enzyme Binding Site(1)"
                     /ApEinfo_label="BsaI Restriction Enzyme Binding Site"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8..65
                     /locus_tag="sgRNA scaffold"
                     /ApEinfo_label="sgRNA scaffold"
                     /ApEinfo_fwdcolor="#073f80"
                     /ApEinfo_revcolor="#073f80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    66..142
                     /locus_tag="tRNA"
                     /ApEinfo_label="tRNA"
                     /ApEinfo_fwdcolor="#80003f"
                     /ApEinfo_revcolor="#80003f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    615..655
                     /locus_tag="sgRNA scaffold(1)"
                     /ApEinfo_label="sgRNA scaffold"
                     /ApEinfo_fwdcolor="#073f80"
                     /ApEinfo_revcolor="#073f80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 GGTCTCaGCA Agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
       61 ggtgcaacaa agcaccagtg gtctagtggt agaatagtac cctgccacgg tacagacccg
      121 ggttcgattc ccggctggTG CAtGAGACGa gatctagacg accggtggaa agcgggcagt
      181 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      241 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      301 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
      361 gaggacctcg agggggggcc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg
      421 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      481 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      541 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      601 cactagtcgt ctccGTTTta gagctagaaa tagcaagtta aaataaggct aGTCCcGAGA
      661 CCacgaagtg ccgaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
      721 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
      781 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
      841 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
      901 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa
      961 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     1021 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     1081 ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     1141 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     1201 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     1261 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     1321 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     1381 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     1441 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     1501 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     1561 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     1621 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     1681 gatcagttgg aagaatttgt ccattacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     1741 taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
     1801 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
     1861 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgctgtcag
     1921 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1981 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2041 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2101 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2161 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2221 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2281 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2341 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2401 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2461 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2521 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2581 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2641 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2701 tcctgcactc tgt    
//
