LOCUS       pTAG6E                  2810 bp ds-DNA     circular     11-SEP-2021
SOURCE      
  ORGANISM  
COMMENT     pAGM9121 from 1 to 2659
COMMENT     
COMMENT     No SpeI, BglII, or RV sites in it.
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             830..1837
                     /vntifkey="4"
                     /locus_tag="spec\orf?"
                     /ApEinfo_label="spec\orf?"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2170..2784
                     /vntifkey="21"
                     /locus_tag="rep\-\pMB1"
                     /ApEinfo_label="rep\-\pMB1"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      2105..2787
                     /locus_tag="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    286..561
                     /vntifkey="21"
                     /locus_tag="lacZ'"
                     /ApEinfo_label="lacZ'"
                     /ApEinfo_fwdcolor="#333333"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        164..285
                     /vntifkey="30"
                     /locus_tag="lacZ\promoter"
                     /ApEinfo_label="lacZ\promoter"
                     /ApEinfo_fwdcolor="#933500"
                     /ApEinfo_revcolor="#933500"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(125..130)
                     /locus_tag="Esp3I recognition site"
                     /ApEinfo_label="Esp3I recognition site"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             493..561
                     /locus_tag="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    246..268
                     /locus_tag="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    672..748
                     /locus_tag="tRNA"
                     /ApEinfo_label="tRNA"
                     /ApEinfo_fwdcolor="#80003f"
                     /ApEinfo_revcolor="#80003f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    596..671
                     /locus_tag="sgRNA scaffold"
                     /ApEinfo_label="sgRNA scaffold"
                     /ApEinfo_fwdcolor="#073f80"
                     /ApEinfo_revcolor="#073f80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1..6
                     /locus_tag="BsaI Restriction Enzyme Binding Site"
                     /ApEinfo_label="BsaI Restriction Enzyme Binding Site"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(754..759)
                     /locus_tag="BsaI Restriction Enzyme Binding Site(1)"
                     /ApEinfo_label="BsaI Restriction Enzyme Binding Site"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8..46
                     /locus_tag="sgRNA scaffold(1)"
                     /ApEinfo_label="sgRNA scaffold"
                     /ApEinfo_fwdcolor="#073f80"
                     /ApEinfo_revcolor="#073f80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    47..123
                     /locus_tag="tRNA(1)"
                     /ApEinfo_label="tRNA"
                     /ApEinfo_fwdcolor="#80003f"
                     /ApEinfo_revcolor="#80003f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    589..594
                     /locus_tag="Esp3I recognition site(1)"
                     /ApEinfo_label="Esp3I recognition site"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 GGTCTCaGTC Cgttatcaac ttgaaaaagt ggcaccgagt cggtgcaaca aagcaccagt
       61 ggtctagtgg tagaatagta ccctgccacg gtacagaccc gggttcgatt cccggctggT
      121 GCATGAGACG agatctagac gaccggtgga aagcgggcag tgagcgcaac gcaattaatg
      181 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
      241 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
      301 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggaggacctc gagggggggc
      361 ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
      421 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      481 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      541 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg ccactagtCG TCTCcGTTTt
      601 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
      661 cgagtcggtg caacaaagca ccagtggtct agtggtagaa tagtaccctg ccacggtaca
      721 gacccgggtt cgattcccgg ctggtgcaGC TTcGAGACCa cgaagtgccg aggcacgaac
      781 ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg
      841 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
      901 ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
      961 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
     1021 cagtcgccct aaaacaaagt taaacatcat gggggaagcg gtgatcgccg aagtatcgac
     1081 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     1141 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagcgata ttgatttgct
     1201 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     1261 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     1321 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     1381 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     1441 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     1501 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     1561 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     1621 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     1681 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     1741 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
     1801 ttacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt
     1861 caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa
     1921 ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata
     1981 agccctacac aaattgggag atatatcatg ctgtcagacc aagtttactc atatatactt
     2041 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     2101 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2161 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2221 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2281 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2341 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2401 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2461 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2521 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2581 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2641 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2701 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2761 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tgcactctgt 
//
