LOCUS       pMC_1_CmYLCV_6          2716 bp ds-DNA     circular     11-SEP-2021
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...ab3oaoi-7n
VERSION     urn.local...ab3oaoi-7n
KEYWORDS    .
SOURCE      
  ORGANISM   .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             926..1717
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /locus_tag="SmR"
                     /ApEinfo_label="SmR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1888..2476
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        12..476
                     /created_by="User"
                     /locus_tag="CmYLCV promoter"
                     /ApEinfo_label="CmYLCV promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1812..2494
                     /locus_tag="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1..6
                     /locus_tag="BsaI Restriction Enzyme Binding Site"
                     /ApEinfo_label="BsaI Restriction Enzyme Binding Site"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(482..487)
                     /locus_tag="BsaI Restriction Enzyme Binding Site(1)"
                     /ApEinfo_label="BsaI Restriction Enzyme Binding Site"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ggtctcaGGA Gtggcagaca tactgtccca caaatgaaga tggaatctgt aaaagaaaac
       61 gcgtgaaata atgcgtctga caaaggttag gtcggctgcc tttaatcaat accaaagtgg
      121 tccctaccac gatggaaaaa ctgtgcagtc ggtttggctt tttctgacga acaaataaga
      181 ttcgtggccg acaggtgggg gtccaccatg tgaaggcatc ttcagactcc aataatggag
      241 caatgacgta agggcttacg aaataagtaa gggtagtttg ggaaatgtcc actcacccgt
      301 cagtctataa atacttagcc cctccctcat tgttaaggga gcaaaatctc agagagatag
      361 tcctagagag agaaagagag caagtagcct agaagtagtc aaggcggcga agtattcagg
      421 cacgtggcca ggaagaagaa aagccaagac gacgaaaaca ggtaagagct aagcttAATG
      481 tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata
      541 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      601 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      661 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa
      721 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
      781 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
      841 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
      901 tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca
      961 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     1021 tttgtacggc tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt
     1081 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     1141 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     1201 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     1261 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     1321 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     1381 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     1441 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     1501 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     1561 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     1621 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta
     1681 cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata
     1741 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1801 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1861 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1921 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1981 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     2041 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2101 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2161 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2221 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2281 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2341 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2401 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2461 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2521 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2581 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2641 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     2701 tcattaatca ctctgt    
//
