LOCUS       pMOD_D4800EC            3781 bp ds-DNA     circular     15-SEP-2021
DEFINITION  GG3 vector for Gibson assembly of donor template and subsequent
            Golden gate assembly with the TALENs into viral vectors for GT.
ACCESSION   urn.local...1405530994060.76
VERSION     urn.local...1405530994060.76
KEYWORDS    .
SOURCE      
  ORGANISM   .
COMMENT     
COMMENT     Note - lacZ orientation is unknown - it could have gone in to the
            EcoRV site in the opposite orientation.
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    join(2927..3781,1..1)
                     /locus_tag="High Copy Ori"
                     /ApEinfo_label="High Copy Ori"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     cds             complement(210..515)
                     /%_Identity=100
                     /Motif="ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTT
                     TGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTG
                     GCCAGTGCACGTCTGCTGTCAGATAAAGTCCCCCGTGAACTTTACCCGGTGGTGCATA
                     TCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGT
                     TATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATT
                     AACCTGATGTTCTGGGGAATATAA"
                     /annotation_group="ccdB: 228 <- 533"
                     /modified_by="User"
                     /Transferred_From="pTRANS_240 - to be verified by
                     sequencing (pTC200)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ccdB"
                     /ApEinfo_label="ccdB"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     5'utr           complement(516..834)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /annotation_group="ccdB 5' UTR: 534 <- 852; ccdb 5' UTR:
                     5,473 <- 5,791"
                     /modified_by="User"
                     /%_Identity=100
                     /annotation_group="ccdb 5' UTR: 5,473 <- 5,791"
                     /modified_by="User"
                     /X-Transferred_From="pMOD_B2000; pMOD_B2101; pMOD_B2103;
                     pMOD_B2103b; pMOD_B2109; pMOD_B2112; pMOD_B2203;
                     pMOD_B2301; pMOD_B2303; pMOD_B2309; pMOD_B2312;
                     pMOD_B2403; pMOD_B2515; pMOD_B2517; pMOD_B2518;
                     pMOD_B2519; pMOD_B2520; pMOD_B2615; pTRANS_100;
                     pTRANS_110; pTRANS_120; pTRANS_130"
                     /X-Transferred_Similarity="100.00%; 99.69%"
                     /Transferred_From="pTRANS_100, pTRANS_110, pTRANS_120,
                     pTRANS_130, pTRANS_150, pTRANS_160, pTRANS_240 - to be
                     verified by sequencing (pTC200)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ccdB 5' UTR"
                     /ApEinfo_label="ccdB 5' UTR"
                     /ApEinfo_fwdcolor="#ffd327"
                     /ApEinfo_revcolor="#ffd327"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            complement(835..1515)
                     /modified_by="User"
                     /X-Transferred_From="pTRANS_100; pTRANS_110; pTRANS_120;
                     pTRANS_130"
                     /X-Transferred_Similarity="99.85%"
                     /%_Identity=100
                     /Motif="ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCA
                     TCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACC
                     GTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTT
                     ATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTAT
                     GGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTT
                     TTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCC
                     GGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTA
                     TTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGT
                     TTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCA
                     CCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGT
                     TCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAG
                     TACTGCGATGAGTGGCAGGCGGGGCGTAATCTAGAGGATCCGGCTTACTAA"
                     /annotation_group="CmR: 853 <- 1,533"
                     /Transferred_From="pTRANS_100, pTRANS_110, pTRANS_120,
                     pTRANS_130, pTRANS_150, pTRANS_160, pTRANS_240 - to be
                     verified by sequencing (pTC200)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="CmR"
                     /ApEinfo_label="CmR"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1..7
                     /locus_tag="AarI"
                     /ApEinfo_label="AarI"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#ff6fcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1784..1790)
                     /locus_tag="AarI(1)"
                     /ApEinfo_label="AarI"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#ff6fcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    12..15
                     /locus_tag="AarI overhang"
                     /ApEinfo_label="AarI overhang"
                     /ApEinfo_fwdcolor="#000080"
                     /ApEinfo_revcolor="#000080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1776..1779
                     /locus_tag="AarI overhang(1)"
                     /ApEinfo_label="AarI overhang"
                     /ApEinfo_fwdcolor="#000080"
                     /ApEinfo_revcolor="#000080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1079..1084)
                     /locus_tag="Esp3I recognition site"
                     /ApEinfo_label="Esp3I recognition site"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(295..300)
                     /locus_tag="BsaI Binding site"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1759..1764
                     /locus_tag="Esp3I recognition site(1)"
                     /ApEinfo_label="Esp3I recognition site"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(29..34)
                     /locus_tag="Esp3I recognition site(2)"
                     /ApEinfo_label="Esp3I recognition site"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1806..2859
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7ffe07"
                     /ApEinfo_revcolor="#7ffe07"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cacctgcaac gTCCCggcgc gccCTATcga gacgGATATC ggtgaccact ttgtacaaga
       61 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
      121 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg tcgacctgca
      181 gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg
      241 tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc
      301 ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc
      361 gggtaaagtt cacgggggac tttatctgac agcagacgtg cactggccag ggggatcacc
      421 atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg
      481 ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc
      541 aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc
      601 tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc
      661 ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact
      721 gtaatacgct gcttcatagc atacctcttt ttgacatact tcgggtatac atatcagtat
      781 atattcttat accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta
      841 agccggatcc tctagattac gccccgcctg ccactcatcg cagtactgtt gtaattcatt
      901 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
      961 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     1021 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     1081 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     1141 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     1201 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     1261 ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag
     1321 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     1381 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag cgactgactg
     1441 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     1501 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat cctaactcaa
     1561 aatccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgcggc
     1621 cgccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     1681 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc ttttttgtac
     1741 aaacttgtca ccGATATCcg tctccGTTAg agctcAGTGa ctagcaggtg atgaaaagcc
     1801 ttagggtcag gtggcacttt tcggggaaat gtgcgatgaa cccctatttg tttatttttc
     1861 taaatacatt caaatatgta tccgctaatg agacaataac cctgataaat gcttcaatta
     1921 tattgaaaaa ggaagagtat gagtatccaa catttccgtg tcgcccttat tccctttttt
     1981 gcggccttct gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     2041 gaagatcagt tgggtgcccg agtgggttac attgaactgg atctcaacag cggcaaaatc
     2101 ttagagagtt ttcgccccga agaacgcttc ccaatgatga gcactttcaa agttctgcta
     2161 tgtggcgcgg tgttatcccg tattgatgct gggcaagagc aactcggtcg ccgcatacac
     2221 tattctcaga atgacttggt tgagtattca ccagtcacag aaaagcatct tacggatggc
     2281 atgacagtaa gagaattatg tagtgctgcc ataaccatga gtgataacac tgcggccaac
     2341 ttacttctga caacgattgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     2401 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     2461 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaactc tccgcaaact attaactggc
     2521 gaactactta ctcttgcttc acgccagcaa ctcattgact ggatggaggc ggacaaagtt
     2581 gcaggaccac ttctgcgctc ggcacttccg gctggctggt ttattgctga taaatctgga
     2641 gccggtgagc gtggCtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     2701 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     2761 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacga agggaaataa
     2821 atagtagccc gcctgatgtg cgggcttttt tttggtggtg gttaccagtg tcgcttagct
     2881 accgtaggtc ctcttaggcg gtccgattag cgacatagtc atcaatgcct tgacggcaaa
     2941 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3001 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3061 ctaccagcgg tggtttgttt gccggatcaa gagctactaa ctctttttcc gaaggtaact
     3121 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3181 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3241 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3301 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgct cacagcccag cttggagcga
     3361 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3421 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3481 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3541 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3601 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3661 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3721 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3781 c     
//
