LOCUS       pMOD_A5801_Luc_         4808 bp ds-DNA     circular     15-SEP-2021
DEFINITION  MODULE A with 35S:AtCas9:HSPt.
ACCESSION   urn.local...8k-6a8icnw
VERSION     urn.local...8k-6a8icnw
KEYWORDS    .
SOURCE      
  ORGANISM   .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      1..857
                     /modified_by="User"
                     /locus_tag="High Copy Ori"
                     /ApEinfo_label="High Copy Ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        879..1720
                     /modified_by="User"
                     /locus_tag="35S promoter"
                     /ApEinfo_label="35S promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      3390..3639
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="ATATGAAGATGAAGATGAAATATTTGGTGTGTCAAATAAAAAGCTTGTGT
                     GCTTAAGTTTGTGTTTTTTTCTTGGCTTGTTGTGTTATGAATTTGTGGCTTTTTCTAA
                     TATTAAATGAATGTAAGATCTCATTATAATGAATAAACAAATGTTTCTATAATCCATT
                     GTGAATGTTTTGTTGGATCTCTTCTGCAGCATATAACTACTGTATGTGCTATGGTATG
                     GACTATGGAATATGATTAAAGATAAG"
                     /annotation_group="ATATGAAGATGAAGATGAAATATTTGGTGTGTCAAATAA
                     AAAGCTTGTGTGCTTAAGTTTGTGTTTTTTTCTTGGCTTGTTGTGTTATGAATTTGTG
                     GCTTTTTCTAATATTAAATGAATGTAAGATCTCATTATAATGAATAAACAAATGTTTC
                     TATAATCCATTGTGAATGTTTTGTTGGATCTCTTCTGCAGCATATAACTACTGTATGT
                     GCTATGGTATGGACTATGGAATATGATTAAAGATAAG: 9,412 -> 9,661"
                     /modified_by="User"
                     /locus_tag="HSP terminator"
                     /ApEinfo_label="HSP terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            3688..4741
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3656..3659
                     /locus_tag="AarI overhang"
                     /ApEinfo_label="AarI overhang"
                     /ApEinfo_fwdcolor="#000080"
                     /ApEinfo_revcolor="#000080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1729..1731
                     /locus_tag="start"
                     /ApEinfo_label="start"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    867..870
                     /locus_tag="AarI overhang(1)"
                     /ApEinfo_label="AarI overhang"
                     /ApEinfo_fwdcolor="#000080"
                     /ApEinfo_revcolor="#000080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    856..862
                     /locus_tag="AarI"
                     /ApEinfo_label="AarI"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#ff6fcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3664..3670)
                     /locus_tag="AarI(1)"
                     /ApEinfo_label="AarI"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#ff6fcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4536..4541
                     /locus_tag="BsaI Binding site"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1729..3381
                     /locus_tag="Firefly Luciferase (Luc+)"
                     /ApEinfo_label="Firefly Luciferase (Luc+)"
                     /ApEinfo_fwdcolor="#0f7ffe"
                     /ApEinfo_revcolor="#0f7ffe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3379..3381
                     /locus_tag="stop"
                     /ApEinfo_label="stop"
                     /ApEinfo_fwdcolor="#fb0106"
                     /ApEinfo_revcolor="#fb0106"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatGATC ggcgcgccag atttgccttt tcaatttcag
      901 aaagaatgct aacccacaga tggttagaga ggcttacgca gcaggtatca tcaagacgat
      961 ctacccgagc aataatctcc aggaaatcaa ataccttccc aagaaggtta aagatgcagt
     1021 caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc tcaagatcag
     1081 aagtactatt ccagtatgga cgattcaagg cttgcttcac aaaccaaggc aagtaataga
     1141 gattggagtc tctaaaaagg tagttcccac tgaatcaaag gccatggagt caaagattca
     1201 aatagaggac ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt
     1261 acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta
     1321 ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca
     1381 aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt
     1441 gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
     1501 catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
     1561 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat
     1621 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
     1681 ataaggaagt tcatttcatt tggagagaac acgggggact cctgcaggat ggaagacgcc
     1741 aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag
     1801 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
     1861 gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca
     1921 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
     1981 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
     2041 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc
     2101 gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca
     2161 atcatccaaa aaattattat catggattct aaaacggatt accagggatt tcagtcgatg
     2221 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag
     2281 tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg
     2341 cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat
     2401 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
     2461 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta
     2521 atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt
     2581 gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac
     2641 gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg
     2701 gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag
     2761 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     2821 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     2881 aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat
     2941 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct
     3001 tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac
     3061 aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc
     3121 ttcgacgcTg gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt
     3181 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt
     3241 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa
     3301 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
     3361 ggcggaaaga tcgccgtgtg acgtcgacga tatgaagatg aagatgaaat atttggtgtg
     3421 tcaaataaaa agcttgtgtg cttaagtttg tgtttttttc ttggcttgtt gtgttatgaa
     3481 tttgtggctt tttctaatat taaatgaatg taagatcaca ttataatgaa taaacaaatg
     3541 tttctataat ccattgtgaa tgttttgttg gatctcttct gcagcatata actactgtat
     3601 gtgctatggt atggactatg gaatatgatt aaagataagc cagagctctg gtgacGGACg
     3661 tgggcaggtg tgatgaaaag ccttagggtc aggtggcact tttcggggaa atgtgcgatg
     3721 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctaa tgagacaata
     3781 accctgataa atgcttcaat tatattgaaa aaggaagagt atgagtatcc aacatttccg
     3841 tgtcgccctt attccctttt ttgcggcctt ctgccttcct gtttttgctc acccagaaac
     3901 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgcc cgagtgggtt acattgaact
     3961 ggatctcaac agcggcaaaa tcttagagag ttttcgcccc gaagaacgct tcccaatgat
     4021 gagcactttc aaagttctgc tatgtggcgc ggtgttatcc cgtattgatg ctgggcaaga
     4081 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtatt caccagtcac
     4141 agaaaagcat cttacggatg gcatgacagt aagagaatta tgtagtgctg ccataaccat
     4201 gagtgataac actgcggcca acttacttct gacaacgatt ggaggaccga aggagctaac
     4261 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4321 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4381 tctccgcaaa ctattaactg gcgaactact tactcttgct tcacgccagc aactcattga
     4441 ctggatggag gcggacaaag ttgcaggacc acttctgcgc tcggcacttc cggctggctg
     4501 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4561 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4621 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4681 actgtcagac gaagggaaat aaatagtagc ccgcctgatg tgcgggcttt tttttggtgg
     4741 tggttaccag tgtcgcttag ctaccgtagg tcctcttagg cggtccgatt agcgacatag
     4801 tcatcaat     
//
